Human cGMP-dependent protein kinase 1(PRKG1) ELISA kit

Instructions
Code CSB-EL018715HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name protein kinase, cGMP-dependent, type I
Alternative Names cGK 1 ELISA Kit; cGK1 ELISA Kit; cGKI alpha ELISA Kit; cGKI ELISA Kit; cGKI beta ELISA Kit; cGMP-dependent protein kinase 1 ELISA Kit; cGMP-dependent protein kinase I ELISA Kit; DKFZp686K042 ELISA Kit; FLJ36117 ELISA Kit; KGP1_HUMAN ELISA Kit; KGPB ELISA Kit; MGC71944 ELISA Kit; pkg ELISA Kit; PRKG1 ELISA Kit; PRKG1B ELISA Kit; PRKGR1A ELISA Kit; PRKGR1B ELISA Kit
Abbreviation PRKG1
Uniprot No. Q13976
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 3.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human PRKG1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 96
Range % 92-100
1:2 Average % 91
Range % 88-95
1:4 Average % 103
Range % 98-107
1:8 Average % 89
Range % 85-96
Recovery
The recovery of human PRKG1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 95 90-100
EDTA plasma (n=4) 87 83-95
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
1000 1.984 2.062 2.023 1.867
500 1.464 1.405 1.435 1.279
250 0.981 1.049 1.015 0.859
125 0.698 0.687 0.693 0.537
62.5 0.471 0.452 0.462 0.306
31.2 0.326 0.341 0.334 0.178
15.6 0.274 0.276 0.275 0.119
0 0.157 0.154 0.156  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Data

Function Serine/threonine protein kinase that acts as key mediator of the nitric oxide (NO)/cGMP signaling pathway. GMP binding activates PRKG1, which phosphorylates serines and threonines on many cellular proteins. Numerous protein targets for PRKG1 phosphorylation are implicated in modulating cellular calcium, but the contribution of each of these targets may vary substantially among cell types. Proteins that are phosphorylated by PRKG1 regulate platelet activation and adhesion, smooth muscle contraction, cardiac function, gene expression, feedback of the NO-signaling pathway, and other processes involved in several aspects of the CNS like axon guidance, hippocampal and cerebellar learning, circadian rhythm and nociception. Smooth muscle relaxation is mediated through lowering of intracellular free calcium, by desensitization of contractile proteins to calcium, and by decrease in the contractile state of smooth muscle or in platelet activation. Regulates intracellular calcium levels via several pathways
Gene References into Functions
  1. our study provides the first genome-wide evidence regarding a mechanism by which traumatic life events interact with human genetic variation of PRKG1 in relation to alcohol misuse PMID: 28265120
  2. Disulfide-activated PKGIalpha regulates cardiac diastolic relaxation and fine-tunes the Frank-Starling response. PMID: 27782102
  3. PRKG1, known to induce apoptosis when activated, is increased in all patient derived glioblastoma spheres PMID: 27564115
  4. redox-mediated guidance required PKG1 and consequently, its deficiency in vivo resulted in defective repair and enhanced neuropathic pain after nerve injury. PKG1-dependent repair functions will outweigh its signaling functions in spinal nociceptive LTP, so that inhibition of PKG1 is no option for neuropathic pain. PMID: 27978504
  5. Oxidant signaling underlies PKGIalpha modulation of Ca2+ sparks and BKCa in myogenically active arterioles PMID: 27729549
  6. These affinity and activation measurements show that beta-phenyl-1,N2-ethenoguanosine 3',5'-monophosphate (PET-cGMP) is most selective for PKG I, whereas 8-(4-chlorophenylthio)guanosine-3'-5'-cyclic monophosphate (8-pCPT-cGMP) is most selective for PKG II. PMID: 28793191
  7. Hence, SO2 exerted its vasodilatory effects at least partly by promoting disulfide-dependent dimerization of sGC and PKG, resulting in an activated sGC/cGMP/PKG pathway in blood vessels. These findings revealed a new mode of action and mechanisms by which SO2 regulated the vascular tone. PMID: 27009048
  8. these data indicate PKG as an intermediary in EGFR-mediated cell death, likely via apoptotic pathway. PMID: 27381222
  9. The heterozygous c.530G>A:p.Arg177Gln PRKG1 variant was associated with early-onset thoracic aortic aneurysm and dissection in a Spanish family. PMID: 27442293
  10. cAMP is a partial agonist for PKG, and we elucidate the mechanism for cAMP partial agonism through the comparative NMR analysis of the apo, cGMP-, and cAMP-bound forms of the PKG cyclic nucleotide-binding domain B. PMID: 26370085
  11. Protein kinase G has a role in regulating production of H2S, which governs oxygen sensing PMID: 25900831
  12. The C42-C42' disulfide bond dramatically stabilizes PRKG1. PMID: 26132214
  13. The Golgi apparatus regulates PKGIalpha compartmentation and proteolysis. PMID: 25855081
  14. Study of genetic heterogeneity in systemic lupus erythematosus, the top associations in European ancestry were protein kinase, cyclic GMP-dependent, type I (PRKG1) rs7897633 (P(Meta) = 2.75 x 10(-8)) and purine nucleoside phosphorylase (PNP) rs1049564 (P(Meta) = 1.24 x 10(-7)). PMID: 25338677
  15. Expression levels of miR-517a-3p and its target mRNA PRKG1 were inversely correlated in NK cells before and after obstetric delivery. PMID: 25273530
  16. miR-20a expression is up-regulated in response to hypoxia in both mouse and human pulmonary arterial smooth muscle cells PMID: 25447536
  17. Data show that functional cGMP/protein kinase G type 1 (PKG1) is expressed in pancreatic adenocarcinoma cells. PMID: 24826884
  18. These findings characterize PKG as a novel regulator of AR-mediated transcription by enhancing AR cofactor p44/WDR77's function. PMID: 23755100
  19. Neutron diffraction reveals hydrogen bonds critical for cGMP-selective activation: insights for cGMP-dependent protein kinase agonist design. PMID: 25271401
  20. Leucine296 and Arginine297 are key residues of PKG I that mediate cGMP selectivity. PMID: 24239458
  21. These data suggest that increased PKG II activity attenuates bFGFinduced proliferation and migration by inhibiting the MAPK/ERK signaling pathway, whereas PKG I does not PMID: 23404188
  22. nitration of Tyr(247) and the attenuation of cGMP binding is an important mechanism regulating in PKG-1alpha activity and SMC proliferation/differentiation PMID: 24469460
  23. Authors show for the first time that PKG is also able to stably interact with a viral substrate, West Nile virus NS5, in cell culture and in vitro. PMID: 23876037
  24. These findings indicate that PRKG1 risk alleles associate with salt-sensitivity related to a loss of the inhibitory control of renal Na(+) reabsorption, suggestive of a blunt pressure-natriuresis response. PMID: 24060892
  25. left ventricular systolic radial function was associated with common polymorphisms in PRKG1. PMID: 24060898
  26. Recurrent gain-of-function mutation in PRKG1 causes thoracic aortic aneurysms and acute aortic dissections. PMID: 23910461
  27. activation of PKGI with cGMP regulated SSG1 intracellular distribution. PMID: 23831687
  28. ANP attenuates the inflammatory actions of histamine via endothelial GC-A/cGMP/cGKI signaling and inhibitory phosphorylation of TRPC6 channels. PMID: 23814119
  29. The data suggest that PKGIbeta enhances breast cancer cell motility and invasive capacity, at least in part, by phosphorylating CaD. PMID: 23418348
  30. PKG positively regulates proteasome activities and proteasome-mediated degradation of misfolded proteins, likely through posttranslational modifications to proteasome subunits. PMID: 23770744
  31. overexpression of PCs, furin and PC5, but not PC7, which are all expressed in SMC, increase PKGI cleavage in a dose-dependent manner PMID: 23686857
  32. The cyclic GMP-dependent kinase Ialpha -induced RhoA activation is dependent on the activity of ROCK and serine/threonine phosphatases. PMID: 23195829
  33. Studies indicate the presence of the switch helix (SW), a novel motif that promotes the formation of a domain-swapped dimer in the asymmetric unit. PMID: 23416533
  34. Low myocardial PKG activity in heart failure with preserved ejection fraction was associated with raised cardiomyocyte F(passive) and was related to increased myocardial nitrosative/oxidative stress. PMID: 22806632
  35. RhoA is a PKGIalpha target and direct binding of activated PKGIalpha to RhoA is central to cGMP-mediated inhibition of the VSMC Rho kinase contractile pathway PMID: 23066013
  36. Downregulation of PKG1 was associated with malignant and benign breast tumors. PMID: 22791569
  37. These results suggest that DAPK2 is one of the targets of cGK-I in apoptosis induction. PMID: 22580283
  38. PKGIbeta inhibited cell growth and inducd apoptosis in breast cancer cells. PMID: 22095901
  39. cGMP-dependent protein kinase (PKG) Ialpha transfection significantly attenuated the hypoxia-induced down-regulation of the expressions of SM-alpha-actin, MHC and calponin. PMID: 22293199
  40. found in smooth muscle wall of blood vessels transversing the clitoral supepithelial and stromal space PMID: 21697861
  41. Cyclic 3',5'-guanosine monophosphate-dependent protein kinase inhibits colon cancer cell adaptation to hypoxia. PMID: 21563174
  42. cGMP dependent kinase 1 alpha interacts with and phosphorylates transient receptor potential cation channel subfamily C member 7 PMID: 21402151
  43. data suggest that activation of PKG signaling in pericytes inhibits focal adhesion formation by inhibiting Rac1 PMID: 21451103
  44. NO, depending on its concentration, may act in human PBMCs as a stimulator of IL-6 expression and modulator of NF-KB involving the sGC/cGMP/PKG pathway PMID: 21414799
  45. The mechanism of cyclic nucleotide binding to PKG was investigated by determining crystal structures of the amino-terminal cyclic nucleotide-binding domain (CNBD-A) of human PKG I bound to either cGMP or cAMP. PMID: 21526164
  46. The novel cGMP/PKG/ROS/calmodulin/CaMKII signaling pathway may regulate cardiomyocyte excitability by opening K(ATP) channels and contribute to cardiac protection against ischemia-reperfusion injury. PMID: 21479273
  47. These findings provide evidence of a physical and compartmentalized association between SERT and PKGIalpha that supports. PMID: 19656393
  48. A novel mechanism has been elucidated that is able to increase PKG-I protein content in contractile cells, with hypotensive consequences in rat thoracic aorta. PMID: 21160032
  49. disruption of the IP3R1-IRAG calcium regulation system is a novel cause of dysfunctional osteoclasts unrelated to defects in attachment proteins or acid secretion PMID: 20567233
  50. structure provides molecular details of this unique leucine/isoleucine zipper, revealing specific hydrophobic and ionic interactions that mediate dimerization and demonstrating the topology of the GKAP interaction surface. PMID: 20826808

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Involvement in disease Aortic aneurysm, familial thoracic 8 (AAT8)
Subcellular Location Cytoplasm
Protein Families Protein kinase superfamily, AGC Ser/Thr protein kinase family, cGMP subfamily
Tissue Specificity Primarily expressed in lung and placenta.
Database Links

HGNC: 9414

OMIM: 176894

KEGG: hsa:5592

UniGene: Hs.407535

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