Human choline acetyltransterase(ChAT)ELISA Kit

Code CSB-E17522h
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P28329
Lead Time 3-5 working days
Abbreviation CHAT
Protein Biological Process 1 Neurobiology
Target Name choline acetyltransferase
Alias CMS1A, CMS1A2, acetyl CoA:choline O-acetyltransferase
Species Homo sapiens (Human)
Protein Biological Process 3 Neurotransmitter biosynthesis
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 3.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Neuroscience
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This gene encodes an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer disease. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform.
HGNC 1912
RGD 1304627
MGI 88392
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human ChAT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %90
Range %86-93
1:2Average %101
Range %96-104
1:4Average %92
Range %86-95
1:8Average %95
Range %91-98
Recovery
The recovery of human ChAT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9589-98
EDTA plasma (n=4)10193-104
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
10001.934 1.952 1.943 1.788
5001.290 1.267 1.279 1.124
2500.738 0.754 0.746 0.591
1250.427 0.412 0.420 0.265
62.50.306 0.301 0.304 0.149
31.250.241 0.255 0.248 0.093
15.60.201 0.188 0.195 0.040
00.157 0.153 0.155
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Function Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.
Involvement in disease Myasthenic syndrome, congenital, 6, presynaptic (CMS6)
Protein Families Carnitine/choline acetyltransferase family
Database Links

HGNC: 1912

OMIM: 118490

KEGG: hsa:1103

STRING: 9606.ENSP00000337103

UniGene: Hs.302002

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