Human cluster of differentiation 19(CD19) ELISA Kit

Code CSB-E14094h
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P15391
Lead Time 3-5 working days
Abbreviation CD19
Target Name CD19 molecule
Alias B4, MGC12802, B-lymphocyte antigen CD19|CD19 antigen
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. This gene encodes a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.
HGNC 1633
RGD 1309086
MGI 88319
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human CD19 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 91
Range % 84-96
1:2 Average % 99
Range % 93-104
1:4 Average % 95
Range % 89-99
1:8 Average % 93
Range % 86-97
Recovery
The recovery of human CD19 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 94 86-98
EDTA plasma (n=4) 88 82-91
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
10 2.223 2.350 2.287 2.144
5 1.567 1.652 1.610 1.467
2.5 1.084 1.002 1.043 0.900
1.25 0.679 0.694 0.687 0.544
0.625 0.463 0.474 0.469 0.326
0.312 0.338 0.327 0.333 0.190
0.156 0.207 0.212 0.210 0.067
0 0.142 0.143 0.143  
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Function Assembles with the antigen receptor of B-lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.
Involvement in disease Immunodeficiency, common variable, 3 (CVID3)
Subcellular Location Membrane, Single-pass type I membrane protein
Database Links

HGNC: 1633

OMIM: 107265

KEGG: hsa:930

STRING: 9606.ENSP00000437940

UniGene: Hs.652262

Pathway PI3K-Akt signaling pathway
B cell receptor signaling pathway
Hematopoietic cell lineage

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