Human cluster of differentiation 30(CD30) ELISA Kit

Code CSB-E17502h
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P28908
Lead Time 3-5 working days
Abbreviation TNFRSF8
Target Name tumor necrosis factor receptor superfamily, member 8
Alias CD30, D1S166E, Ki-1, CD30 antigen|CD30L receptor|Ki-1 antigen|cytokine receptor CD30|lymphocyte activation antigen CD30
Species Homo sapiens (Human)
Sample Types serum, plasma, cell culture supernates
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 3.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This protein is a member of the TNF-receptor superfamily. This receptor is expressed by activated, but not by resting, T and B cells. TRAF2 and TRAF5 can interact with this receptor, and mediate the signal transduction that leads to the activation of NF-kappaB. This receptor is a positive regulator of apoptosis, and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
HGNC 11923
RGD 3879
MGI 99908
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human CD30 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:100Average %90
Range %84-96
1:200Average %97
Range %91-105
1:400Average %103
Range %97-107
1:800Average %89
Range %83-94
Recovery
The recovery of human CD30 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 10094-105
EDTA plasma (n=4)106101-110
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
10002.811 2.762 2.787 2.661
5002.578 2.402 2.490 2.364
2502.166 1.997 2.082 1.956
1251.312 1.355 1.334 1.208
62.50.645 0.652 0.649 0.523
31.20.398 0.395 0.397 0.271
15.60.223 0.236 0.230 0.104
00.127 0.125 0.126
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Function Receptor for TNFSF8/CD30L. May play a role in the regulation of cellular growth and transformation of activated lymphoblasts. Regulates gene expression through activation of NF-kappa-B.
Subcellular Location Isoform 1: Cell membrane, Single-pass type I membrane protein, SUBCELLULAR LOCATION: Isoform 2: Cytoplasm
Database Links

HGNC: 11923

OMIM: 153243

KEGG: hsa:943

STRING: 9606.ENSP00000263932

UniGene: Hs.1314

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