Human cluster of differentiation 30(CD30) ELISA Kit

Instructions
Code CSB-E17502h
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application

Product Details

Target Name tumor necrosis factor receptor superfamily, member 8
Alternative Names CD30, D1S166E, Ki-1, CD30 antigen|CD30L receptor|Ki-1 antigen|cytokine receptor CD30|lymphocyte activation antigen CD30
Abbreviation TNFRSF8
Uniprot No. P28908
Species Homo sapiens (Human)
Sample Types serum, plasma, cell culture supernates
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 3.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human CD30 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:100Average %90
Range %84-96
1:200Average %97
Range %91-105
1:400Average %103
Range %97-107
1:800Average %89
Range %83-94
Recovery
The recovery of human CD30 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 10094-105
EDTA plasma (n=4)106101-110
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
10002.811 2.762 2.787 2.661
5002.578 2.402 2.490 2.364
2502.166 1.997 2.082 1.956
1251.312 1.355 1.334 1.208
62.50.645 0.652 0.649 0.523
31.20.398 0.395 0.397 0.271
15.60.223 0.236 0.230 0.104
00.127 0.125 0.126
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days
Still Have Questions? Leave a Message or Start an on-line Chat

Target Data

Function Receptor for TNFSF8/CD30L. May play a role in the regulation of cellular growth and transformation of activated lymphoblasts. Regulates gene expression through activation of NF-kappa-B.
Subcellular Location Isoform 1: Cell membrane, Single-pass type I membrane protein, SUBCELLULAR LOCATION: Isoform 2: Cytoplasm
Database Links

HGNC: 11923

OMIM: 153243

KEGG: hsa:943

STRING: 9606.ENSP00000263932

UniGene: Hs.1314

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