Human cluster of differentiation 45(CD45) ELISA Kit

Unavailable
Code CSB-E17497h
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P08575
Lead Time 3-5 working days
Abbreviation PTPRC
Target Name protein tyrosine phosphatase, receptor type, C
Alias B220, CD45, CD45R, GP180, LCA, LY5, T200, CD45 antigen|T200 glycoprotein|T200 leukocyte common antigen|glycoprotein|leukocyte-common antigen|protein tyrosine phosphatase, receptor type, c polypeptid
Species Homo sapiens (Human)
Sample Types serum, plasma, cell culture supernates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This protein is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus belongs to receptor type PTP. This gene is specifically expressed in hematopoietic cells. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Four alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported.
HGNC 9666
RGD 3451
MGI 97810
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human CD45 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:5 Average % 89
Range % 85-93
1:10 Average % 105
Range % 101-109
1:20 Average % 83
Range % 80-86
1:40 Average % 87
Range % 84-90
Recovery
The recovery of human CD45 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 93 90-96
EDTA plasma (n=4) 105 101-109
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
10 2.703 2.768 2.736 2.569
5 1.989 1.968 1.979 1.812
2.5 1.283 1.248 1.266 1.099
1.25 0.762 0.768 0.765 0.598
0.625 0.474 0.498 0.486 0.319
0.312 0.343 0.365 0.354 0.187
0.156 0.286 0.278 0.282 0.115
0 0.169 0.164 0.167  
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Function Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity (By similarity).
Involvement in disease Severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)B(+)NK(+) SCID); Multiple sclerosis (MS)
Subcellular Location Membrane, Single-pass type I membrane protein, Membrane raft
Protein Families Protein-tyrosine phosphatase family, Receptor class 1/6 subfamily
Database Links

HGNC: 9666

OMIM: 126200

KEGG: hsa:5788

STRING: 9606.ENSP00000356346

UniGene: Hs.654514

Pathway Fc gamma R-mediated phagocytosis
T cell receptor signaling pathway

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