Human coupling factor 6(CF6)ELISA Kit

Instructions
Code CSB-E13880h
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name ATP synthase, H+ transporting, mitochondrial F0 complex, subunit F6
Alternative Names ATP synthase; H+ transporting; mitochondrial F0 complex; subunit F6 ELISA Kit; ATP synthase-coupling factor 6; mitochondrial ELISA Kit; ATP synthase-coupling factor 6; mitochondrial ELISA Kit; ATP5 ELISA Kit; ATP5A ELISA Kit; ATP5J ELISA Kit; ATP5J_HUMAN ELISA Kit; ATPase subunit F6 ELISA Kit; ATPM ELISA Kit; CF6 ELISA Kit; F6 ELISA Kit
Abbreviation ATP5J
Uniprot No. P18859
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 3.12 ng/mL-200 ng/mL
Sensitivity 0.78 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human CF6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %95
Range %85-102
1:2Average %92
Range %85-101
1:4Average %97
Range %86-108
1:8Average %89
Range %83-100
Recovery
The recovery of human CF6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9583-102
EDTA plasma (n=4)9686-106
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
2002.352 2.417 2.385 2.248
1001.702 1.782 1.742 1.605
501.193 1.123 1.158 1.021
250.678 0.684 0.681 0.544
12.50.406 0.416 0.411 0.274
6.250.265 0.277 0.271 0.134
3.120.232 0.228 0.230 0.093
00.135 0.139 0.137
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Target Data

Function Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements. Also involved in the restoration of oligomycin-sensitive ATPase activity to depleted F1-F0 complexes.
Gene References into Functions
  1. AKT2 and XIST expression was identified as a potential biomarker participating in the effect of ATP5J in colorectal cancer PMID: 29484395
  2. CF6-induced increase in apoptotic cells was blocked by immature or mature IF1, being accompanied by protein kinase B (PKB) phosphorylation. IF1 antagonizes the pro-apoptotic action of CF6 by relief of intracellular acidification and resultant phosphorylation of PKB. PMID: 26659871
  3. Over-expression of the ATP5J gene correlates with cell migration and 5-fluorouracil sensitivity in colorectal cancer. PMID: 24124598
  4. CF6 plays a crucial role in the development of insulin resistance and hypertension PMID: 22038518
  5. The phenotypic range of retinal, peripheral and central nervous system disease expression is characterized in a single family with NARP syndrome from the ATPase 6 m.8993T>C mtDNA point mutation. PMID: 20953793
  6. coupling factor 6 induces the development of systolic dysfunction and upregulation of nicotinamide adenine dinucleotide phosphate oxidase in the heart under the high-salt diet PMID: 20811295
  7. CF6 is a novel risk factor for ischemic heart disease in end-stage renal disease. Synergism of this peptide and asymmetric dimethylarginine might contribute to its occurrence presumably by inhibition of prostacyclin and nitric oxide production. PMID: 14633154
  8. Mutation analysis revealed the T9176C mutation in the mtATPase 6 gene (OMIM 516060) and the mutation load was above 90% in the patients with Leigh syndrome. PMID: 15709156
  9. Plasma CF6 elevated in patients with acute myocardial infarction. At 3 days, plasma CF6 correlated positively with plasma creatinine kinase peak value and correlated negatively with left ventricular ejection fraction. PMID: 15785006
  10. T8993G allele causes severe extrapyramidal dysfunction and Leigh disease but there is no correlation between the degree of enzyme deficiency and the severity of the phenotype. PMID: 16532470
  11. Increased CF6 may be responsible in part for decreased prostacyclin observed in coronary heart disease, in particular after PTCA and stent therapy. Potential risk factor for coronary heart disease. PMID: 17456993
  12. in vascular endothelial cells both CF6 (coupling factor 6) and Angiotensin II downregulate PECAM-1 (platelet/endothelial cell adhesion molecule) expression via activation of c-Src kinase PMID: 18243211
  13. Coupling factor 6 enhances Src-mediated responsiveness to angiotensin II in resistance arterioles and cells. PMID: 19106112

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Subcellular Location Mitochondrion, Mitochondrion inner membrane
Protein Families Eukaryotic ATPase subunit F6 family
Database Links

HGNC: 847

OMIM: 603152

KEGG: hsa:522

STRING: 9606.ENSP00000389649

UniGene: Hs.246310

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