Human forkhead box O1,FOXO1 ELISA Kit

Unavailable
Code CSB-E11175h
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. Q12778
Lead Time 3-5 working days
Abbreviation FOXO1
Protein Biological Process 1 Transcription/Transcription regulation
Target Name forkhead box O1
Alias FKH1, FKHR, FOXO1A, forkhead box O1A (rhabdomyosarcoma)|forkhead homolog in rhabdomyosarcoma|forkhead, Drosophila, homolog of, in rhabdomyosarcoma
Species Homo sapiens (Human)
Protein Biological Process 3 Transcription
Sample Types serum, plasma, cell lysates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Epigenetics and Nuclear Signaling
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma.
HGNC 3819
RGD 620283
MGI 1890077
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human FOXO1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %94
Range %84-102
1:2Average %97
Range %91-108
1:4Average %95
Range %86-104
1:8Average %95
Range %90-100
Recovery
The recovery of human FOXO1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9589-102
EDTA plasma (n=4)9795-101
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
101.991 2.005 1.998 1.899
51.706 1.720 1.713 1.614
2.51.361 1.348 1.355 1.256
1.250.999 0.964 0.982 0.883
0.6250.711 0.729 0.720 0.621
0.3120.513 0.522 0.518 0.419
0.1560.279 0.285 0.282 0.183
00.098 0.100 0.099
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Function Transcription factor that is the main target of insulin signaling and regulates metabolic homeostasis in response to oxidative stress. Binds to the insulin response element (IRE) with consensus sequence 5'-TT[G/A]TTTTG-3' and the related Daf-16 family binding element (DBE) with consensus sequence 5'-TT[G/A]TTTAC-3'. Activity suppressed by insulin. Main regulator of redox balance and osteoblast numbers and controls bone mass. Orchestrates the endocrine function of the skeleton in regulating glucose metabolism. Acts synergistically with ATF4 to suppress osteocalcin/BGLAP activity, increasing glucose levels and triggering glucose intolerance and insulin insensitivity. Also suppresses the transcriptional activity of RUNX2, an upstream activator of osteocalcin/BGLAP. In hepatocytes, promotes gluconeogenesis by acting together with PPARGC1A and CEBPA to activate the expression of genes such as IGFBP1, G6PC and PCK1. Important regulator of cell death acting downstream of CDK1, PKB/AKT1 and SKT4/MST1. Promotes neural cell death. Mediates insulin action on adipose tissue. Regulates the expression of adipogenic genes such as PPARG during preadipocyte differentiation and, adipocyte size and adipose tissue-specific gene expression in response to excessive calorie intake. Regulates the transcriptional activity of GADD45A and repair of nitric oxide-damaged DNA in beta-cells. Required for the autophagic cell death induction in response to starvation or oxidative stress in a transcription-independent manner. Mediates the function of MLIP in cardiomyocytes hypertrophy and cardiac remodeling (By similarity).
Involvement in disease Rhabdomyosarcoma 2 (RMS2)
Subcellular Location Cytoplasm, Nucleus
Tissue Specificity Ubiquitous.
Database Links

HGNC: 3819

OMIM: 136533

KEGG: hsa:2308

STRING: 9606.ENSP00000368880

UniGene: Hs.370666

Pathway Cellular senescence
AGE-RAGE signaling pathway in diabetic complications
AMPK signaling
FoxO signaling pathway
Glucagon signaling pathway
Insulin signaling pathway

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