Human hepatic lipase,HL ELISA Kit

Instructions
Code CSB-E10031h
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name hepatic lipase,HL
Alternative Names LIPC ELISA Kit; HTGL ELISA Kit; Hepatic triacylglycerol lipase ELISA Kit; HL ELISA Kit; Hepatic lipase ELISA Kit; EC 3.1.1.3 ELISA Kit; Lipase member C ELISA Kit
Abbreviation HL
Uniprot No. P11150
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.156 U/mL-10 U/mL
Sensitivity 0.039 U/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human HL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %96
Range %93-99
1:2Average %85
Range %82-90
1:4Average %87
Range %83-92
1:8Average %100
Range %97-103
Recovery
The recovery of human HL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9996-102
EDTA plasma (n=4)8481-87
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
U/mlOD1OD2AverageCorrected
102.408 2.469 2.439 2.306
51.965 1.999 1.982 1.849
2.51.211 1.268 1.240 1.107
1.250.676 0.698 0.687 0.554
0.6250.395 0.378 0.387 0.254
0.3120.282 0.273 0.278 0.145
0.1560.197 0.187 0.192 0.059
00.132 0.134 0.133
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function Hepatic lipase has the capacity to catalyze hydrolysis of phospholipids, mono-, di-, and triglycerides, and acyl-CoA thioesters. It is an important enzyme in HDL metabolism. Hepatic lipase binds heparin.
Gene References into Functions
  1. The downregulation of LIPC in the HepG2 cells was associated with the decreased expression of CD133, decreased cell proliferation and colony formation, as well as increased resistance to chemotherapy. PMID: 30015857
  2. APOB polymorphism rs679899 is associated with type 2 diabetes and glutamyl transpeptidase levels, while the LIPC polymorphism rs6083 may influence plasma lipid levels in Chinese Han population. PMID: 29883758
  3. The expression of hHL promoted hepatic triglyceride accumulation and de novo lipogenesis without affecting triglyceride secretion, and this was associated with an upregulation of Srebf1 as well as the main genes controlling the synthesis of fatty acids. Transgenic mice also exhibited more adiposity and an increased LPL-mediated FFA influx into the WAT without affecting glucose tolerance PMID: 29244870
  4. LPL is important for the maturation of small discoidal HDL particles into large spherical HDL particles, while HL is important for HDL remodeling of very large HDL particles into intermediate-size HDL particles, as shown in lipoprotein lipase and hepatic lipase deficiency PMID: 27573733
  5. The expression level of LIPC, SLC16A8, and TIMP-3 was significantly associated with age-related macular degeneration pathology. PMID: 27966779
  6. Hepatic lipase activity increases after levothyroxine therapy in subclincal hypothyroidism and can be interpreted as a possible explanation for the decrease in remnant-like lipoprotein cholesterol. PMID: 26839156
  7. The hepatic lipase (LIPC) rs10468017 variant was associated with a significantly decreased risk of AMD PMID: 26503844
  8. polymorphisms in HL protect HIV-infected patients from developing atherogenic dyslipidemia in a dose-dependent manner PMID: 26101956
  9. the association between LIPC C-514T polymorphism, obesity and plasma lipid profile in Chinese children and adolescents, is reported. PMID: 26282880
  10. results show that SM is a physiological inhibitor of HL activity in lipoproteins and that the specificity of the enzyme towards HDL is at least partly due to its low SM content. PMID: 26193433
  11. The role of hepatic triglyceride lipase in remnant lipoprotein metabolism. PMID: 25239670
  12. Dietary fat intake significantly modified the LIPC genetic effect on changes in serum triglyceride, LDL cholesterol, and HDL cholesterol concentrations during a 2-y randomized weight-loss intervention trial. PMID: 25926410
  13. Hepatic lipase C-514T gene polymorphism is associated with cardiometabolic parameters and cardiovascular risk factors but not with fatty liver PMID: 25550127
  14. there was no strong evidence to support an association between LIPC and HDCP in Han Chinese women. However, LIPC variants were associated with BMI, SBP, and lipid profiles in patients. PMID: 25117371
  15. The present study suggested that the LIPC-514 C/T polymorphism of the HL gene has no significant association with the risk of endometriosis in the studied Iranian women. PMID: 24124978
  16. Our meta-analysis indicates that LIPC rs10468017 variant is associated with a reduced risk of advanced age-related macular degeneration. PMID: 25010633
  17. The findings suggest that LIPC and LPL genes could both modify the risk for AMD and the metabolism of lutein and zeaxanthin. PMID: 24223199
  18. Results indicate the spatial consensus catalytic triad "Ser-Asp-His", a characteristic motif in lipoprotein lipase, hepatic lipase, and endothelial lipase. PMID: 23991054
  19. HL activity, as TG-hydrolase, was increased in metabolic syndrome and obesity; directly correlated with low-density lipoprotein-cholesterol and apolipoprotein B and negatively with high-density lipoprotein-cholesterol in controls. PMID: 24458708
  20. The -514T LIPC allele is associated with CAD under normotriglyceridemic conditions and constitutes an independent determinant of pejorative ABI in coronary patients. PMID: 23874450
  21. Some patients with homozygous c.736T>A can have a mild hypotrichosis phenotype with long hairs in adulthood PMID: 22449147
  22. study extends the spectrum of mutations in LPAR6/P2RY5 gene and underscores those mutations in LPAR6/P2RY5 and LIPH result in similar phenotypes PMID: 22385360
  23. Patients who are carrying of the -250A allele in the promoter of the LIPC gene are susceptible to type 2 diabetes mellitus in Chinese Han population. PMID: 23351562
  24. Low intralesional expression of LIPC correlates with prolonged post-operative cisplatin-mediated survival. PMID: 23343765
  25. this preliminary study showed that the gene variants of ABCA1R219K and LIPC-250 G/A might influence AD susceptibility in South Chinese Han population, but the polymorphism of CETPTaq1B didn't show any association PMID: 23181436
  26. The LIPC (hepatic lipase) promoter variants were associated with advanced macular degenration, confirming that LIPC polymorphisms may be a genetic risk factor for advanced age-related macular degeneration in the Caucasian population. PMID: 23348725
  27. The high carbohydrate diet induces the beneficial effect of the CC genotype of hepatic lipase rs1800588 polymorphism on the apoB100/apoAI ratio only in the males in a young and healthy Chinese cohort. PMID: 22935046
  28. The decreased apoB100/apoAI ratio is associated with the A allele of hepatic lipase G-250A polymorphism only in young Chinese males. PMID: 23457133
  29. Hepatic lipase plays an important role in determining the size and density of LDL and HDL particles. [Review] PMID: 21986251
  30. Papillomavirus DNA integration is associated with loss of LIPC gene and thus cervical carcinogenesis. PMID: 22262398
  31. Common and rare genetic variants in CETP and LIPC, but not LIPG, were more commonly found in the Thai HALP group, which could potentially contribute to high high-density lipoprotein cholesterol phenotypes in this population. PMID: 22464213
  32. Single-nucleotide polymorphism in lipase is associated with colorectal cancer. PMID: 22294764
  33. the LIPC -514C>T variant significantly affects training-induced anti-atherogenic changes in VLDL-TG, VLDL particles, and HDL through an association with increased LPL activity in CC subjects PMID: 21960661
  34. Avascular necrosis of femoral head is not associated with rs59644784 and rs1800588 SNPs. PMID: 21261091
  35. presence of the B1B1 Taq1B CETP genotype contributes to the presence of diabetes, independently of age, sex, BMI and waist. However, among carriers of B1B1, the presence of GG genotype of the -250 LIPC polymorphism increases this risk further PMID: 22073289
  36. The variation in lipid profile associated with the C-514T in hepatic lipase polymorphism is significant, and the T allele is associated with the best response to estrogen replacement therapy PMID: 22047520
  37. Data show a novel pathway involving HL hydrolysis of VLDL that activates PPARdelta through generation of specific monounsaturated fatty acids. PMID: 21750705
  38. Suggest that smaller LDL size, which is associated with higher levels of triglycerides and CETP and the hepatic lipase CC genotype, may serve as a risk factor for coronary artery disease in hemodialysis patients. PMID: 21352420
  39. CETP and LIPC polymorphisms influence the respective hepatic transcript levels, demonstrate interactions on HDL cholesterol and suggest that imbalances between CETP and LIPC activities may modulate the risk of carotid atherosclerosis PMID: 21371711
  40. Polymorphisms in APOA5 (-1131T > C, S19W), PPARgamma (Pro12Ala) and hepatic lipase (-514C > T) were studied in 1492 subjects from the New Delhi Birth Cohort (NDBC). PMID: 21548985
  41. Genotyping of 25 single nucleotide polymorphisms covering the complete LIPC gene and haplotypic analysis reveal no association of LIPC with Alzheimer's disease. PMID: 19155102
  42. LIPC and ABCA1 are related to intermediate and large drusen, as well as advanced age related macular degeneration PMID: 21447678
  43. The effects of common variants in the LPL, LIPC, and CETP genes on HDL-C levels are modified by physical activity. PMID: 21252145
  44. HL expression in McA-RH7777 cells result in secretion of [(3)H]TAG-poor VLD PMID: 21189265
  45. These results demonstrated that fatty acid synthase and hepatic lipase might be FXR-regulated genes in liver cells. PMID: 20373033
  46. LIPC is associated with reduced risk of advanced age-related macular degeneration. PMID: 21139980
  47. relationship between plasma angiopoietin-like protein 3 (ANGPTL3), and lipoprotein lipase (LPL) activity and hepatic triglyceride lipase PMID: 20595410
  48. Report significant interactions between the hepatic lipase -514 C/T promoter polymorphism, hypercholesterolemia, waist circumference and risk of myocardial infarction. PMID: 19695855
  49. genetic association studies in Iranian population: carriers of T allele in -514C/T polymorphism (an SNP in the promoter region) in LIPC gene have higher HDL-cholesterol than noncarriers PMID: 20822508
  50. The HDL-raising allele of the LIPC gene (T) was associated with a reduced risk of AMD (age-related macular degeneration). PMID: 20888482

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Involvement in disease Hepatic lipase deficiency (HL deficiency)
Subcellular Location Secreted
Protein Families AB hydrolase superfamily, Lipase family
Database Links

HGNC: 6619

OMIM: 125853

KEGG: hsa:3990

STRING: 9606.ENSP00000299022

UniGene: Hs.654472

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