Human hepatic lipase,HL ELISA Kit

Code CSB-E10031h
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P11150
Lead Time 3-5 working days
Abbreviation HL
Target Name hepatic lipase,HL
Alias N/A
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.156 U/mL-10 U/mL
Sensitivity 0.039 U/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of human HL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
1:1Average %96
Range %93-99
1:2Average %85
Range %82-90
1:4Average %87
Range %83-92
1:8Average %100
Range %97-103
The recovery of human HL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9996-102
EDTA plasma (n=4)8481-87
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
102.408 2.469 2.439 2.306
51.965 1.999 1.982 1.849
2.51.211 1.268 1.240 1.107
1.250.676 0.698 0.687 0.554
0.6250.395 0.378 0.387 0.254
0.3120.282 0.273 0.278 0.145
0.1560.197 0.187 0.192 0.059
00.132 0.134 0.133
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Function Hepatic lipase has the capacity to catalyze hydrolysis of phospholipids, mono-, di-, and triglycerides, and acyl-CoA thioesters. It is an important enzyme in HDL metabolism. Hepatic lipase binds heparin.
Involvement in disease Hepatic lipase deficiency (HL deficiency)
Subcellular Location Secreted
Protein Families AB hydrolase superfamily, Lipase family
Database Links

HGNC: 6619

OMIM: 125853

KEGG: hsa:3990

STRING: 9606.ENSP00000299022

UniGene: Hs.654472

Pathway Cholesterol metabolism

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