Human heterogeneous nuclear ribonucleoprotein A1(hnRNP A1) ELISA Kit

Instructions
Code CSB-E17318h
Size 96T,5×96T,10×96T
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Product Details

Target Name heterogeneous nuclear ribonucleoprotein A1
Alternative Names HNRNPA 1 ELISA Kit; Helix destabilizing protein ELISA Kit; Helix-destabilizing protein ELISA Kit; Heterogeneous nuclear ribonucleoprotein A1 ELISA Kit; Heterogeneous nuclear ribonucleoprotein A1B protein ELISA Kit; Heterogeneous nuclear ribonucleoprotein B2 protein ELISA Kit; Heterogeneous nuclear ribonucleoprotein core protein A1 ELISA Kit; hnRNP A1 ELISA Kit; hnRNP core protein A1 ELISA Kit; HNRNPA1 ELISA Kit; HNRPA1 ELISA Kit; MGC102835 ELISA Kit; Nuclear ribonucleoprotein particle A1 protein ELISA Kit; ROA1_HUMAN ELISA Kit; Single strand DNA binding protein UP1 ELISA Kit; Single strand RNA binding protein ELISA Kit; Single-strand RNA-binding protein ELISA Kit
Abbreviation HNRNPA1
Uniprot No. P09651
Species Homo sapiens (Human)
Sample Types serum, plasma, cell culture supernates
Detection Range 0.625 ng/mL-40 ng/mL
Sensitivity 0.156 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human HNRNP A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 104
Range % 100-107
1:2 Average % 97
Range % 95-100
1:4 Average % 91
Range % 85-96
1:8 Average % 94
Range % 89-99
Recovery
The recovery of human HNRNP A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 102 98-104
EDTA plasma (n=4) 91 83-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
40 2.779 2.822 2.801 2.660
20 2.444 2.502 2.473 2.332
10 1.987 1.912 1.950 1.809
5 1.123 1.120 1.122 0.981
2.5 0.679 0.659 0.669 0.528
1.25 0.452 0.470 0.461 0.320
0.625 0.289 0.279 0.284 0.143
0 0.136 0.145 0.141  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Target Data

Function Involved in the packaging of pre-mRNA into hnRNP particles, transport of poly(A) mRNA from the nucleus to the cytoplasm and may modulate splice site selection
Gene References into Functions
  1. Moreover, SRSF10, hnRNP A1/A2 and Sam68 collaborate to drive the DNA damage-induced splicing response of several transcripts that produce components implicated in apoptosis, cell-cycle control and DNA repair. PMID: 29396485
  2. By using an integrative structural biology approach, we show that hnRNP A1 forms a 1:1 complex with pri-mir-18a where both RNA recognition motifs (RRMs) bind to cognate RNA sequence motifs in the terminal loop of pri-mir-18a. PMID: 29946118
  3. Data suggest that one way heterogeneous nuclear ribonucleoprotein A1 (HNRNPA1) regulates exon definition is to modulate the interaction of U2 small nuclear RNA auxiliary factor 2 (U2AF2) with decoy or bona fide 3' splice site (3'ss). PMID: 29650551
  4. Our results indicate that hnRNP A1 promotes cell survival and cell cycle progression following UVB radiation PMID: 29229447
  5. These findings indicated that hnRNPA1 is highly expressed in gastric cancer (GC)and promoted invasion by inducing Epithelialtomesenchymal transition (EMT) in GC cells. Thus, hnRNPA1 may be a potential therapeutic target for GC. PMID: 29484423
  6. A significant overlap between hnRNPA1 and DDX5 splicing targets and they share many closely linked binding sites. PMID: 30042133
  7. Results suggest that telomeric repeat-containing RNA (TERRA) regulates binding of heterogeneous nuclear ribonucleoprotein A1 to the telomere in a region surrounding the telomere, leading to a deeper understanding of the mechanism of TERRA function. PMID: 27958374
  8. mutation in HNRNPA1 located in the nuclear localization signal domain of hnRNPA1, enhances the recruitment of mutant hnRNPA1 into stress granules, indicating that an altered nuclear localization signal activity plays an essential role in amyotrophic lateral sclerosis pathogenesis. PMID: 29033165
  9. Rules of RNA specificity of hnRNP A1 revealed by global and quantitative analysis of its affinity distribution have been described. PMID: 28193894
  10. study showed that UVB induces alternative splicing of hdm2 by increasing the expression and the binding of hnRNP A1 to hdm2 full-length mRNA PMID: 26757361
  11. The authors show by NMR spectroscopy that both RNA recognition motifs of hnRNP A1 can bind simultaneously to a single bipartite motif of the human intronic splicing silencer ISS-N1, which controls survival of motor neuron exon 7 splicing. PMID: 28650318
  12. current knowledge of the involvement of hnRNPA1 in cancer, including its roles in regulating cell proliferation, invasiveness, metabolism, adaptation to stress and immortalization. PMID: 28791797
  13. Case Report: inflammatory myofibroblastic tumor (IMT) of the urinary bladder with a novel HNRNPA1-ALK fusion. PMID: 28504207
  14. EGF signaling upregulates an E3 ubiquitin (Ub) ligase adaptor, SPRY domain-containing SOCS box protein 1 (SPSB1), which recruits Elongin B/C-Cullin complexes to conjugate lysine 29-linked poly Ubiquitin chains onto hnRNP A1. PMID: 28084329
  15. hnRNP A1 directly binds to the 5' untranslated region of the RON mRNA and activates its translation through G-quadruplex RNA secondary structures PMID: 26930004
  16. We demonstrate that the wild-type sequence harbors an hnRNP A1 and hnRNP A2/B1-binding exonic splicing silencer (ESS) overlapping the 5'splice site (5'ss) that prevents pseudoexon inclusion.we demonstrate that splice switching oligonucleotide (SSO) mediated blocking of the pseudoexon 3'ss and 5'ss effectively restores normal GLA splicing PMID: 27595546
  17. The hnRNP A1 binding map can be used to identify potential targets for splice-switching oligonucleotides-based therapy. PMID: 27380775
  18. Biophysical titrations reveal that the 5'-AUAGC-3' bulge undergoes a conformational change to assemble a functional hnRNP A1-human enterovirus 71 RNA complex. PMID: 28625847
  19. PRMT5 regulates internal ribosome entry site-dependent translation via methylation of hnRNP A1. PMID: 28115626
  20. KRAS-E2F1-ILK-hnRNPA1 regulatory loop enables pancreatic cancer cells to promote oncogenic KRAS signaling and to interact with the tumor microenvironment to promote aggressive phenotypes. PMID: 26616862
  21. findings suggest that a new player, i.e., O-GlcNAcylation, regulates hnRNP A1 translocation and interaction with Trn1, possibly affecting its function PMID: 27913144
  22. The results of this study suggest that hnRNPA1 is the causative gene in the family with flail arm ALS. This further expanded the disease phenotype of hnRNPA1 mutations. PMID: 27694260
  23. Introduction of point mutations into the hnRNP A1-binding site or knockdown of hnRNP A1 expression promoted human papillomavirus 18 233;416 splicing and reduced E6 expression. PMID: 27489271
  24. Studies provides evidence that hnRNP A1 is a new autoantigen of Behcet's Disease and associated with deep vein thrombosis. PMID: 27211563
  25. these data suggest that the level of hnRNPA1 is strictly controlled to be within a certain range by the mRNA autoregulation in the physiological condition so that the cytotoxicity-causative alteration of hnRNPA1 expression does not take place. PMID: 28000042
  26. The CCAT1/miR-490/hnRNPA1 axis promotes gastric cancer migration, and it may have a possible diagnostic and therapeutic potential in gastric cancer. PMID: 26825578
  27. Data show that heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) unfolds the HRAS protein i-motif. PMID: 26674223
  28. We demonstrate that an hnRNPA1 and CBX5 bi-directional core promoter fragment does not comprise intrinsic capacity for specific CBX5 down-regulation in metastatic cells PMID: 26791953
  29. The sST2-5'UTR contains an IRES element, which is activated by a MEK/ERK-dependent increase in cytoplasmic localization of hnRNP A1 in response to FGF2, enhancing the translation of sST2. PMID: 27168114
  30. hnRNP A1 is implicated in the free fatty acid-induced expression of SREBP-1a and of its target genes as well as in the lipid accumulation in hepatocytes. PMID: 26869449
  31. analysis of HIV-1 intron splicing silencer and its interactions with the UP1 domain of hnRNPA1 PMID: 26607354
  32. Our results indicate that the knockdown of hnRNP A1 has a specific function on the splicing of CD44 in breast cancer cells. PMID: 26151392
  33. HNRNPA1 might play an important role in lung adenocarcinoma cells. PMID: 26581508
  34. MiR-26a and mir-584 inhibit the binding of hnRNP A1-CDK6 mRNA and induce colorectal cancer cell apoptosis. PMID: 26494299
  35. NF-kappaB2/p52:c-Myc:hnRNPA1 Pathway Regulates Expression of Androgen Receptor Splice Variants and Enzalutamide Sensitivity in Prostate Cancer PMID: 26056150
  36. lowering the levels of hnRNP A1/A2 elicits defective transcription elongation on a fraction of P-TEFb-dependent genes, hence favoring the transcription of P-TEFb-independent genes PMID: 26011126
  37. In neuroblastoma cells, hnRNP A1 directly interacts with Drp1 mRNA at its 3'UTR region, and enhances translation potential without affecting mRNA stability. PMID: 26518267
  38. The disordered regions of key RNP granule components and the full-length granule protein hnRNPA1 can phase separate in vitro, producing dynamic liquid droplets. PMID: 26412307
  39. ALS-linked mutations in ubiquilin-2 or hnRNPA1 reduce interaction between ubiquilin-2 and hnRNPA1 PMID: 25616961
  40. The marked loss of hnRNPA1 in motor neurons with concomitant cytoplasmic aggregation of TDP-43 may represent a severe disturbance of mRNA processing, suggesting a key role in progressive neuronal death in amyotrophic lateral sclerosis . PMID: 25338872
  41. These findings suggested that hnRNP A1 plays key roles in the regulation of cell cycle progression and pathogenesis of Oral squamous cell carcinoma . PMID: 25752295
  42. Its mutation is proved to be a rare cause of amyotrophic lateral sclerosis, frontotemporal dementia, and inclusion body myopathy. in the nertherlands. PMID: 24612671
  43. miR-18a induces the apoptosis of colon cancer cells by directly binding to oncogenic hnRNP A1. PMID: 24166503
  44. Its mutation is rare in patients with frontotemporal lobar degeneration. PMID: 24119545
  45. Data reveal three distinct enthalpic contributions from the interactions of hnRNP Al (UP1) with the Na+ form of G-quadruplex DNA. PMID: 24831962
  46. findings show HNRNPA1 modulates expression of an alternatively spliced transcript of HMGCR by regulating splicing and altering RNA stability, resulting in reduced HMGCR enzyme activity and increased LDL-Cholesterol uptake; results suggest HNRNPA1 plays a role in the variation of cardiovascular disease risk and statin response PMID: 24001602
  47. HNRNPA1 is a novel transcriptional regulator of IL-6 expression, acting via the 5'-flanking sequence of the gene. PMID: 23985572
  48. The results describe a UP1 binding mechanism that is likely different from current models used to explain the alternative splicing properties of hnRNP A1. PMID: 24628426
  49. This functional hnRNP A1 deletion mutant is similar to a predicted hnRNP A1 isoform, which had not been previously experimentally characterized. PMID: 24530421
  50. The results of this study suggested that mutations in hnRNPA1, A2/B1, and A3 genes are a rare finding in amyotrophic lateral sclerosis. PMID: 23827524
  51. hnRNP A1 interacts with exon 5 to promote distal exon 6 inclusion of Fas pre-mRNA. PMID: 23430061
  52. HnRNP A1/A2 and SF2/ASF regulate alternative splicing of interferon regulatory factor-3 and affect immunomodulatory functions in human non-small cell lung cancer cells. PMID: 23658645
  53. HCC patients with high hnRNP A1 tended to have higher levels of CD44v6. PMID: 22821376
  54. Heat-induced changes of HuR and of hnRNP A1, causes the proteins to exit the nucleus and co-localize to large cytoplasmic foci that represent heat-induced stress granules. PMID: 22983828
  55. analysis of several exons regulated by hnRNP L shows a clear relationship between the potential for binding of hnRNP A1 and U1 snRNA and the effect of hnRNP L on splicing PMID: 23394998
  56. definition of pathogenic mutations in PrLDs of heterogeneous nuclear ribonucleoproteins (hnRNPs) A2B1 and A1 in families with inherited degeneration affecting muscle, brain, motor neuron and bone, and in one case of familial amyotrophic lateral sclerosis PMID: 23455423
  57. Data indicate that heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) participates in the IRES (internal ribosome entry site) -dependent translation of sterol-regulatory-element-binding protein 1 (SREBP-1a) mRNA through RNA-protein interaction. PMID: 23106379
  58. These findings present cholinergic-mediated hnRNP A/B loss and impaired RNA metabolism as important mechanisms involved in Alzheimer's disease. PMID: 22628224
  59. hnRNPA2/B1 facilitates recognition of the p21-specific promoter by the small activating double-stranded RNA. PMID: 23035981
  60. hnRNP A1 is preferentially phosphorylated by atrial natriuretic peptide signaling which leads to co-localization of cGMP kinase and hnRNP A1 to the nucleus. PMID: 22285803
  61. These data point to a functional interaction between hnRNP F and SRSF1 as a mutant that eliminates SRSF1 binding to exon 11, or a SRSF1 knockdown, which prevents the stimulatory effect of hnRNP F over expression. PMID: 22132154
  62. hnRNP A1 forms a ternary complex with the U2AF heterodimer on AG-containing/uridine-rich RNAs, while it displaces U2AF from non-AG-containing/uridine-rich RNAs, an activity that requires the glycine-rich domain of hnRNP A1 PMID: 22325350
  63. These data suggest that MNK1 regulates the phosphorylation and the subcellular distribution of hnRNP A1 and that MNK1 may play a role in the induction of senescence PMID: 22227431
  64. Data show that hnRNPA1/A2, HuR and DAZAP1 splicing factors and DHX36 RNA helicase bind to the ISE, with hnRNPA1 acting negatively and DAZAP1 positively on splicing selection. PMID: 21858080
  65. hnRNP-A1 and its transcription regulatory factor miR-590-3p are disregulated in patients with Alzheimer disease, and the hnRNP-A1 rs7967622 C/C genotype is likely a risk factor for frontotemporal lobar degeneration in male populations. PMID: 21548758
  66. Mechanistic control of carcinoembryonic antigen-related cell adhesion molecule-1 (CEACAM1) splice isoforms by the heterogeneous nuclear ribonuclear proteins hnRNP L, hnRNP A1, and hnRNP M. PMID: 21398516
  67. the phosphorylation status of hnRNP A1 serine 199 regulates the AKT-dependent sensitivity of cells to rapamycin and functionally links IRES-transacting factor annealing activity to cellular responses to mTOR complex 1 inhibition. PMID: 21454539
  68. showed that hnRNP A1 regulates the processing of pri-miR-18a by binding to its terminal loop and reshaping its stem-loop structure, thus allowing for a more effective Drosha cleavage (REVIEW) PMID: 21627027
  69. data suggest that hnRNPA1, TERRA and POT1 act in concert to displace RPA from telomeric ssDNA after DNA replication, and promote telomere capping to preserve genomic integrity PMID: 21399625
  70. SMN2 3' splice site motif functions as a crucial hnRNP A1 binding exonic splicing silencer(ESS), which inhibits inclusion of SMN1/2 exon 7 and is antagonized by the SMN1 exonic splicing enhancer(ESE), but not by the inactive SMN2 sequence. PMID: 21120954
  71. Studies indicate that switching from pyruvate kinase spliced isoform PKM1 to PKM2 promotes aerobic glycolysis and provides a selective advantage for tumor formation, and the alternative splicing is controlled by hnRNP family members. PMID: 20978194
  72. Over-expression of hnRNP A1 is associated with non-small cell lung cancer. PMID: 20716340
  73. IL-6 activates c-Myc translation in MM cells by inducing A1 phosphorylation and cytoplasmic localization PMID: 20974848
  74. Data suggest that increased expression of hnRNP A1, Ezrin, tubulin beta-2C and Annexin A1 in SLNMM indicate a significantly elevated early colorectal cancer metastasis. PMID: 20872967
  75. hnRNP A1 and KSRP have antagonistic roles in the post-transcriptional regulation of let-7a expression. PMID: 20639884
  76. human gliomas overexpress c-Myc, PTB, hnRNPA1 and hnRNPA2 in a manner that correlates with PKM2 expression PMID: 20010808
  77. splicing repressors hnRNP A1 and A2, as well as the polypyrimidine-tract-binding protein PTB, contribute to control of pyruvate kinase isoform M1 and M2 expression PMID: 20133837
  78. Samples from 10 colorectal patients were analyzed. Of 7 significantly and consistently altered proteins identified, hnRNP A1 was one of the most significantly altered proteins and its overexpression was confirmed using RT-PCR and Western blot analyses. PMID: 19715280
  79. Interactions between different hnRNP proteins bound to distinct locations on a pre-mRNA can change its conformation to affect splicing decisions. PMID: 19926721
  80. Data demonstrate that expression levels of hnRNP A1, Q, K, R, and U influence HIV-1 production by persistently infected astrocytes, linking these hnRNPs to HIV replication. PMID: 19808671
  81. exon identity established through differential antagonism between exonic splicing silencer-bound hnRNP A1 and enhancer-bound SR proteins PMID: 11779509
  82. acts as a potent regulator of G alpha(s) isoform expression PMID: 11825891
  83. hnRNP A1 nucleocytoplasmic shuttling activity is required for normal myelopoiesis and BCR/ABL leukemogenesis. PMID: 11884611
  84. interference with the binding of the human T cell leukemia virus type 1 rex regulatory protein to its response element PMID: 11893730
  85. hnRNP A1/A2 binding sites control 5' splice site selection in the hnRNP A1 mRNA precursor PMID: 12060656
  86. High-affinity hnRNP A1 binding sites and duplex-forming inverted repeats have similar effects on 5' splice site selection in support of a common looping out and repression mechanism. PMID: 12212851
  87. A Janus splicing regulatory element modulates HIV-1 tat and rev mRNA production by coordination of hnRNP A1 cooperative binding PMID: 12419255
  88. hnRNP A1 repressed c-src splicing in vitro. The activity of these exonic splicing regulators, SF2/ASF and hnRNP A1, is linked to the splicing of an exon primarily controlled by intronic factors. PMID: 12612063
  89. role in c-H-ras alternative splicing regulation PMID: 12665590
  90. Specific interaction of HNRPA1 with the -219T allelic form modulates Apolipoprotein E promoter activity. PMID: 12799433
  91. Involved in mRNA processing and mRNA nucleocytoplasmic export. Sequestered in intranuclear aggregates. Oculopharyngeal muscular dystrophy intranuclear inclusions are "poly(A) RNA traps", interfering with RNA export and causing muscle cell death. PMID: 12945950
  92. The expression of A1/A2 proteins is elevated in a variety of human cancers, whereas A1/A2 expression is lower or absent in normal tissues. PMID: 14633690
  93. heterogeneous nuclear ribonucleoprotein A1 function has a role as constitutive KCS element components of the interferon-inducible RNA-dependent protein kinase promoter PMID: 14645369
  94. hnRNP A1 binding to the exonic splicing silencer element inhibits splicing of the upstream intron by directly masking the SC35 binding site PMID: 14703516
  95. hnRNP A1 and ASF/SF2 and SC35 have antagonistic roles in splicing of beta-tropomyosin exon 6B PMID: 15208309
  96. used UP1's novel DNA binding to investigate how RNA recognition motifs bind nucleic acid bases through their highly conserved RNP consensus sequences PMID: 15342234
  97. HNRNPA1 is a novel internal ribosome entry site trans-acting factor that modulates alternative initiation of translation of the fibroblast growth factor 2 mRNA PMID: 15525641
  98. the basal expression of gamma-fibrinogen is regulated by a constitutive transcriptional repressor protein, hnRNP A1, and the decreased binding activity of hnRNP A1 leads to the overexpression of gamma chain in HepG2 cells that overexpress the Bbeta chain PMID: 15671034
  99. These observations provide an insight into a new cellular control of HTLV-1 replication and suggest that hnRNP A1 is likely part of the regulatory mechanisms of the life cycle of this human retrovirus in T cells. PMID: 15703079
  100. nhRNP A1 may contribute to maintenance of telomere repeats in cancer cells with enhanced cell proliferation, or hnRNP A1 quantitative alteration could facilitate colon epithelial cell transformation through transcriptional and translational perturbation PMID: 15703818

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Involvement in disease Inclusion body myopathy with early-onset Paget disease with or without frontotemporal dementia 3 (IBMPFD3); Amyotrophic lateral sclerosis 20 (ALS20)
Subcellular Location Nucleus, Cytoplasm, Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs, Shuttles continuously between the nucleus and the cytoplasm along with mRNA, Component of ribonucleosomes (PubMed:17289661), SUBCELLULAR LOCATION: Cytoplasm
Database Links

HGNC: 5031

OMIM: 164017

KEGG: hsa:3178

STRING: 9606.ENSP00000341826

UniGene: Hs.546261

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