Human high sensitivity C-C motif chemokine 2 (CCL2) ELISA kit

Code CSB-EQ004783HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Description

The human CCL2 ELISA Kit is intended to measure human CCL2 in serum, plasma, or tissue homogenates. This assay uses the bi-antibody sandwich technique and enzyme-substrate chromogenic reaction to quantify human CCL2 concentration. The intensity of the colored product is directly proportional to the concentration of CCL2 present in the sample. This human CCL2 ELISA kit has high specificity and excellent sensitivity.

CCL2 is known for its chemotactic functions and controls the migration and infiltration of monocytes/macrophages. Apart from the chemotactic effect, CCL2 also exerts powerful stimulatory and mitogenic roles on mononuclear cells. CCL2/CCR2 signaling is implicated in regulating macrophage recruitment and polarization during inflammation. CCL2/CCR2 signaling is induced and involved in various neurodegenerative disorders including Alzheimer's disease, multiple sclerosis, and ischemic brain injury, as well as in inflammatory diseases such as atherosclerosis, rheumatoid arthritis, and diabetic nephropathy. CCL2 is also related to the development and progression of several cancer types, including breast, prostate, and ovarian.

Target Name C-C motif chemokine 2
Alternative Names C-C motif chemokine 2 ELISA Kit; CCL2 ELISA Kit; CCL2_HUMAN ELISA Kit; Chemokine (C C motif) ligand 2 ELISA Kit; GDCF 2 ELISA Kit; GDCF-2 ELISA Kit; GDCF2 ELISA Kit; HC11 ELISA Kit; HSMCR30 ELISA Kit; JE ELISA Kit; MCAF ELISA Kit; MCP 1 ELISA Kit; MCP-1 ELISA Kit; MCP1 ELISA Kit; MGC9434 ELISA Kit; Monocyte chemoattractant protein 1 ELISA Kit; Monocyte chemotactic and activating factor ELISA Kit; Monocyte chemotactic protein 1 ELISA Kit; Monocyte secretory protein JE ELISA Kit; SCYA2 ELISA Kit; Small inducible cytokine A2 (monocyte chemotactic protein 1; homologous to mouse Sig je) ELISA Kit; Small inducible cytokine A2 ELISA Kit; Small inducible cytokine subfamily A (Cys Cys); member 2 ELISA Kit; Small-inducible cytokine A2 ELISA Kit; SMC CF ELISA Kit; SMC-CF ELISA Kit; SMCCF ELISA Kit
Abbreviation CCL2
Uniprot No. P13500
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 6.25 pg/mL-400 pg/mL
Sensitivity 1.56 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human CCL2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 84  
Range % 80-92  
1:2 Average % 90  
Range % 85-85  
1:4 Average % 100  
Range % 94-107  
1:8 Average % 93  
Range % 86-98  
Recovery
The recovery of human CCL2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 89-99  
EDTA plasma (n=4) 97 90-102  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
400 2.352 2.212 2.282 2.170  
200 1.692 1.746 1.719 1.607  
100 1.111 1.054 1.083 0.971  
50 0.584 0.609 0.597 0.485  
25 0.379 0.354 0.367 0.255  
12.5 0.253 0.269 0.261 0.149  
6.25 0.192 0.199 0.196 0.084  
0 0.114 0.110 0.112    
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Acts as a ligand for C-C chemokine receptor CCR2. Signals through binding and activation of CCR2 and induces a strong chemotactic response and mobilization of intracellular calcium ions. Exhibits a chemotactic activity for monocytes and basophils but not neutrophils or eosinophils. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.
Gene References into Functions
  1. The expression of CX3CL1 and capital EM, Cyrilliccapital ES, Cyrilliccapital ER, Cyrillic-1 affect vital pulmonary capacity and forced expiratory volume in patients with asthma complicated by diabetes mellitus type 2. PMID: 29461240
  2. Aberrant histone modifications, including acetylation and tri-methylation, were found both in global histone and specific MCP-1 gene locos in monocytes from patients with coronary artery disease PMID: 29609686
  3. Alzheimer disease patients had higher plasma MCP-1 levels compared with mild cognitive impairment patients and controls, and severe AD patients had the highest levels. PMID: 29352259
  4. CCL2 played important roles in regulating platelet function and arterial thrombosis through the PKCalpha-P38MAPK-HSP27 pathway. PMID: 29864522
  5. Studied role of monocyte chemoattractant protein-1 (MCP-1) in migration of monocytes and lymphocytes during nickel induced contact hypersensitivity. PMID: 29132256
  6. High chemokine CCL2 expression is associated with colorectal cancer. PMID: 30419802
  7. NOX4 is induced in early alcoholic liver injury and regulates CCR2/CCL2 mRNA stability thereby promoting recruitment of inflammatory cells and production of proinflammatory cytokines. PMID: 28383062
  8. study confirmed the connection between high uMCP-1 levels and poor prognosis and also disease activity in antineutrophil cytoplasmic autoantibody-Associated Vasculitis (AAV); it also suggests an association of the A/A genotype at position -2518 in the MCP-1 gene and poor prognosis in AAV PMID: 29720895
  9. Given that IL-8, MIP-1beta, and MCP-1 are chemokines that play important roles in recruitment of immunocompetent cells for immune defense and tumor cell clearance, the observed lower levels of these markers with increasing PM2.5 exposure may provide insight into the mechanism by which DEE promotes lung cancer. PMID: 29023999
  10. In lupus nephritis, urinary MCP-1 and TWEAK possess higher correlation coefficients with renal damage and larger areas under ROC curves than other markers for rapid discrimination of severe disease. PMID: 29451067
  11. We propose that endogenous gC1qR/p33 physically interacts with MCP-1 causing stabilization of the MCP-1 protein and stimulation of its activity in human periodontal ligament cells, suggesting a novel gC1qR/p33-mediated pro-inflammatory mechanism of action. PMID: 29358188
  12. berberine inhibited the expression of MCP-1 and IL-8 induced by LPS. PMID: 28852897
  13. In Colombian systemic lupus erythematosus patients, urinary NGAL and MCP-1 in addition to anti-C1q antibodies were useful biomarkers for the identification of renal involvement and discrimination of active lupus nephritis. PMID: 29073812
  14. These results provide evidence that necrotic cells induce the expression of CCL2/MCP-1 and CCL20/MIP-3alpha in glioblastoma cells through activation of NF-kappaB and AP-1 and facilitate the infiltration of microglia into tumor tissues. PMID: 30048972
  15. Serum CCL2 and sICAM-1 concentrations were significantly decreased in CNS tumors in comparison with the comparative group. Among proteins tested in the serum, a higher area under the ROC curve (AUC) revealed CCL2 compared to sICAM-1 in differentiating subjects with CNS brain tumors from non-tumoral subjects. PMID: 29086194
  16. MCP-1 -2518 genotype may influence the outcome of nonsurgical periodontal treatment in aggressive periodontitis patients. PMID: 28427824
  17. Study describes how cysteine-rich 61 (CCN1) promotes monocyte migration by upregulating monocyte chemoattractant protein-1 (CCL2) expression in osteoblasts in rheumatoid arthritis (RA) disease. CCN1 could serve as a potential target for RA treatment. PMID: 28341837
  18. The MCP-1 concentration was positively correlated with blood pressure among smokers. PMID: 29098933
  19. CCL2-2518A/G (rs1024611) polymorphism is significantly associated with risk of gynecological cancer. PMID: 29458367
  20. High MCP1 expression is associated with pancreatic adenocarcinoma. PMID: 29205349
  21. Results provide evidence for MCP-1 involvement in the development of PVVs and indicate that inflammation could be implicated in the pathogenesis of this condition. PMID: 28623996
  22. Study found that the activation of CCR2 by its ligand CCL2 increased the expression of SMO and Gli-1, resulting in Hh pathway activation, epithelial-mesenchymal transition and hepatocellular carcinoma cell invasion. PMID: 29115520
  23. these findings collectively indicate that TGF-beta regulates CCL2 expression in a stage-dependent manner during breast cancer progression PMID: 29107385
  24. Individuals with rs1024611 AG and GG genotypes exhibited significantly higher susceptibility to Diabetic Foot Ulcers. PMID: 29995756
  25. REVIEW: regulation and importance of monocyte chemoattractant protein-1 PMID: 28914666
  26. this study shows that CCL2 promoter hypomethylation is associated with gout risk in Chinese Han male population PMID: 28690186
  27. Results showed that both heterozygous and homozygous genotypes are associated with age-related macular degeneration pathology. Allele frequency analysis showed that 'G' allele is frequent in AMD patients as compared to controls PMID: 29664944
  28. Oxidative stress markers in the pregestational period did not have a predictive value in the recurrent pregnancy loss and repeated implantation failure . CCL2 might be useful in risk prediction PMID: 28707148
  29. Distributions of MCP-1 -2518A/G and VEGF -634C/G polymorphisms are significantly different between type 2 diabetes mellitus and diabetic foot ulcer patients PMID: 29901584
  30. In multivariate analysis, the IL1B rs16944 TT and TNF rs1799964 TC genotypes were significantly associated with intrauterine cytomegalovirus infection. Twenty-two out of 72 congenitally infected newborns had confirmed sensorineural hearing loss. Carriers of CT or TT genotype of CCL2 rs13900 had increased risk of hearing loss at birth and at 6 months of age. PMID: 28501927
  31. Effective IL1beta and CCL2 antagonists are currently in clinical review to treat benign inflammatory disease, and their transition to the cancer clinic could have a rapid impact PMID: 28790030
  32. The genotype and allele frequencies between groups did not show significant differences. However, the GG genotype was the most frequent in children with insulin resistance. The GG genotype was associated with insulin resistance (OR = 2.2, P = 0.03) in a genetic model. Conclusion The -2518 A>G MCP-1 gene polymorphism may be related to the development of insulin resistance in Mexican children. PMID: 29694633
  33. the results of the present study confirmed CCL2 as a direct target of miR-206, and showed that the upregulation of CCL2 caused by the downregulation of miR-206 was responsible for the development of severe HEV71 encephalitis. PMID: 28765968
  34. data disclose the complexity of MCP-1 regulation upon CHIKV infection and point to a crucial role of IFNbeta in the chemokine secretion. PMID: 27558873
  35. The study suggests that MCP-1 (-2518A>G) AG genotype and G allele could be considered as risk factors for susceptibility to ovarian cancer. PMID: 28886321
  36. use of MCP-1/IL-10 ratio in combination with ultrasound findings appears to provide a promising modality for predicting preeclampsia PMID: 28038968
  37. It can be concluded that MCP-1 and CCR2 polymorphisms are not associated with AgP in Turkish population. PMID: 28458180
  38. Clathrithromycin suppressed TLR4-mediated MCP-1 production in human mesangial cells. PMID: 27614743
  39. CCL2 siRNA exhibited effective inhibition of cell proliferation and angiogenesis in the glioma cell line U251. PMID: 28714025
  40. CCL2 in addition to IFN-gamma can be used to differentiate among Mycobacterium tuberculosis infection possibilities. PMID: 28610785
  41. Results indicated that MCP-1 and CCR2 polymorphisms may influence the progression of IgAN, but not increase/decrease its susceptibility. PMID: 27788494
  42. Data suggest that, compared to control individuals, patients with T2DM-Y (young onset type 2 diabetes), NPDR (non-proliferative diabetic retinopathy) and PDR (proliferative diabetic retinopathy) exhibit significantly higher serum levels of MCP-1. This study was conducted in India. PMID: 28336215
  43. The meta-analysis demonstrated that urinary MCP-1 was significantly higher in patients with active LN than in those with inactive LN and control subjects, and the patients with inactive LN showed significantly higher urinary MCP-1 levels than the controls. PMID: 27278779
  44. investigated whether functional polymorphism at MCP-1 regulatory region associates with disease phenotype in Indian systemic lupus erythematosus patients (SLE) patients; findings suggest that -2518G allele of MCP-1 -2518 A/G polymorphism is associated with renal disorders and may influence MCP-1 gene expression among Indian SLE patients PMID: 28433894
  45. joint interaction analysis of ACE I/D and CCL2 C-2518T showed a significantly higher frequency of II genotype of ACE I/D in coexistence with TT genotype of CCL2 C-2518T in Henoch-Schonlein purpura (HSP) patients. PMID: 28691415
  46. EGF up-regulated CCL2 expression in HNSCC cells, which recruited monocytes and turned them into M2-like macrophages, thus forming a positive feedback paracrine loop. PMID: 27888616
  47. CCL2 gene silencing inhibits primary tumor growth and metastasis, associated with a reduction in cancer stem cell renewal and recruitment of M2 macrophages in triple negative breast cancer PMID: 27283985
  48. Mesenchymal stem cells from Ankylosing spondylitis patients secreted more monocyte chemoattractant protein 1 (MCP1) during abnormal osteogenic differentiation. PMID: 27921117
  49. CCL2 polymorphism is associated with susceptibility to latent tuberculous infection in well-defined North-East Thai populations. PMID: 27510253
  50. Meta-analysis suggests that the MCP-1-2518A/G polymorphism is associated with an increased risk of lupus nephritis. PMID: 29390552

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Subcellular Location Secreted.
Protein Families Intercrine beta (chemokine CC) family
Tissue Specificity Expressed in the seminal plasma, endometrial fluid and follicular fluid (at protein level). Expressed in monocytes.
Database Links

HGNC: 10618

OMIM: 158105

KEGG: hsa:6347

STRING: 9606.ENSP00000225831

UniGene: Hs.303649

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