Human hormone sensitive lipase,HSL ELISA Kit

Instructions
Code CSB-E09217h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name lipase, hormone-sensitive
Alternative Names Hormone sensitive lipase ELISA Kit; Hormone sensitive lipase testicular isoform ELISA Kit; Hormone-sensitive lipase ELISA Kit; HSL ELISA Kit; LHS ELISA Kit; Lipase hormone sensitive ELISA Kit; LIPE ELISA Kit; LIPS_HUMAN ELISA Kit
Abbreviation LIPE
Uniprot No. Q05469
Species Homo sapiens (Human)
Sample Types serum, plasma
Detection Range 0.78 mIU/mL-50 mIU/mL
Sensitivity 0.195 mIU/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human HSL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:20 Average % 98
Range % 92-106
1:40 Average % 94
Range % 91-98
1:80 Average % 108
Range % 102-114
1:160 Average % 85
Range % 80-89
Recovery
The recovery of human HSL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 98 94-102
EDTA plasma (n=4) 103 98-108
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
mIU/ml OD1 OD2 Average
50 0.103 0.105 0.104
25 0.169 0.174 0.172
12.5 0.269 0.273 0.271
6.25 0.408 0.412 0.410
3.12 0.629 0.638 0.634
1.56 0.919 0.956 0.938
0.78 1.245 1.342 1.294
0 1.874 1.945 1.910
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production.
Gene References into Functions
  1. The homozygous null LIPE mutation could result in marked inhibition of lipolysis from some adipose tissue depots and thus may induce an extremely rare phenotype of MSL and partial lipodystrophy in adulthood associated with complications of insulin resistance, such as diabetes, hypertriglyceridemia and hepatic steatosis. PMID: 27862896
  2. Despite reductions in intramyocellular lipolysis and HSL expression, overexpression of HSL did not rescue defects in insulin action in skeletal myotubes from obese type 2 diabetic subjects. PMID: 25819461
  3. Identification of a homozygous nonsense variant p.Ala507fsTer563 in hormone sensitive lipase as the likely cause of the lipodystrophy phenotype in siblings. PMID: 25475467
  4. These findings indicate the physiological significance of HSL in adipocyte function and the regulation of systemic lipid and glucose homeostasis and underscore the severe metabolic consequences of impaired lipolysis. PMID: 24848981
  5. Serum triglyceride was significantly up-regulated in men with the (CG + GG) genotype of HSL promoter polymorphism. PMID: 23688034
  6. M. leprae suppresses lipid degradation through inhibition of HSL expression. PMID: 22553833
  7. Enzyme promiscuity in the hormone-sensitive lipase family of proteins. PMID: 21933124
  8. Resveratrol increased adipose triglyceride lipase gene and protein expressions, an effect that was not observed for hormone-sensitive lipase in human SGBS adipocytes. PMID: 21543206
  9. LIPE C-60G variation can inhibit the decrease of LDL-C and the increases of HDL-C and apo A-I in young healthy males, and can inhibit the decrease of LDL-C and the increase of insulin in young healthy females induced by a high-carbohydrate diet. PMID: 21826994
  10. suggests that genetic variation of HSL may be a risk factor for male infertility PMID: 21919688
  11. Data show that hormone-sensitive lipase activity is reduced in adipose tissue of patients with and without diabetes, while lipoprotein lipase activity is reduces only in patients with diabetes. PMID: 20926921
  12. It is concluded that ACTH via the PKA pathway stimulates expression of SF-1, which activates transcription of LIPE presumably by interaction with putative binding sequences within promoter A PMID: 21081692
  13. total lipase, ATGL and HSL activities were higher in visceral white adipose tissue of cancer patients compared with individuals without cancer and higher in cancer patients with cachexia compared with cancer patients without cachexia PMID: 21680814
  14. Data indicate that altered ATGL and HSL expression in skeletal muscle could promote DAG accumulation and disrupt insulin signaling and action. PMID: 21498783
  15. Studies indicate tha HSL is regulated by reversible phosphorylation on five critical residues. PMID: 21241784
  16. Those findings indicate improvement and conservation of lifestyle depending on genetic predisposition in ADIPOQ, PLIN and LIPE should be encouraged. PMID: 20495294
  17. The present study aimed at comparing expression and subcellular distribution of perilipin and hormone-sensitive lipase in two abdominal adipose tissues of lean and obese women. PMID: 20017959
  18. genes of C3, hormone-sensitive lipase, and PPARgamma may exert a modifying effect on lipid and glucose metabolism in familial combined hypersensitivity PMID: 11979403
  19. overexpression of HSL, despite increased lipase activity, does not lead to enhanced lipolysis PMID: 12518034
  20. HSL i6 A5 HOMOZYGOSITY IS A RISK FACTOR FOR BODY FAT ACCUMULATION PMID: 12534454
  21. high concentrations of estradiol significantly increased both hormone-sensitive lipase expression and glycerol release relative to control PMID: 12701046
  22. lipolytic catecholamine resistance of sc adipocytes in polycystic ovary syndrome is probably due to a combination of decreased amounts of beta(2)-adrenergic receptors, the regulatory II beta-component of protein kinase A, and hormone-sensitive lipase PMID: 12727985
  23. High adrenaline levels can stimulate hormone-sensitive lipase(HSL) activity regardless of metabolic milieu. Large increases in adrenaline during exercise are able to further stimulate contraction-induced increase in HSL activity. PMID: 12730334
  24. The presence of a catalytically inactive variant of this enzyme is associated with decreased lipolysis in abdominal subcutaneous adipose tissue of obese subjects PMID: 12765952
  25. role of cyclic GMP in natriuretic peptide-mediated phosphorylation in adipocytes PMID: 12970365
  26. AMPK is a major regulator of skeletal muscle HSL activity that can override beta-adrenergic stimulation PMID: 15231718
  27. catalytic serine of hormone-sensitive lipase is highly reactive and similar behavior was also observed with lipases with no lid domain covering their active site, or with a deletion in the lid domain PMID: 15260473
  28. 5'AMP-activated protein kinase phosphorylates hormone-sensitive lipase on Ser565 in human skeletal muscle during exercise with reduced muscle glycogen. Apparently, HSL Ser565 phosphorylation by AMPK during exercise PMID: 15308678
  29. mechanism of infertility in HSL-deficient males is cell autonomous and resides in postmeiotic germ cells, because HSL expression in these cells is in itself sufficient to restore normal fertility PMID: 15345679
  30. a pre-lipolysis complex containing at least AFABP and HSL exists PMID: 15456755
  31. Basal HSL is decreased in patients with type 2 diabetes mellitus, and this may be a consequence of elevated plasma insulin levels. PMID: 15609025
  32. Perilipin targets a novel pool of lipid droplets for lipolytic attack by hormone-sensitive lipase PMID: 16243839
  33. the HSL C-60G polymorphism is associated with increased waist circumference in non-obese subjects PMID: 16534522
  34. adrenergic stimulation contributes to the increase in HSL activity that occurs in human skeletal muscle in the first minute of exercise at 65% and 90% VO2 peak PMID: 16690773
  35. HSL gene expression shows a regulation according to obesity status and is associated with increased adipose tissue lipase activity. PMID: 16752181
  36. although HSL expression and Ser(659) phosphorylation in skeletal muscle during exercise is sex specific, total muscle HSL activity measured in vitro was similar between sexes PMID: 16822962
  37. maternal type 1 diabetes is associated with TG accumulation and increased EL and HSL gene expression in placenta PMID: 16940551
  38. hormone-sensitive lipase PMID: 17026959
  39. Adipose triglyceride lipase and hormone sensitive lipase are responsible for more than 95% of the triacylglycerol hydrolase activity present in murine white adipose tissue. PMID: 17074755
  40. Adipose triglyceride lipase (ATGL) is less important than hormone-sensitive lipase (HSL) in regulating catecholamine-induced lipolysis. Both lipases regulate basal lipolysis in human adipocytes. ATGL expression is not influenced by obesity or PCOS. PMID: 17327373
  41. In obese subjects, insulin resistance and hyperinsulinemia are strongly associated with ATGL and HSL mRNA and protein expression, independent of fat mass PMID: 17356053
  42. Dydrogesterone and norethisterone increase secretion of HSL from abdominaal adipocytes. PMID: 17587400
  43. variation of the HSL gene might be associated with a physiological effect on in vivo beta-adrenoceptor-mediated fat oxidation PMID: 18249203
  44. Real-time PCR revealed that large adipocytes expressed higher mRNA levels of hormone sensitive lipase. PMID: 18383440
  45. Obesity is accompanied by impaired fasting glycerol release, lower HSL protein expression, and serine phosphorylation. PMID: 18398140
  46. Mapping of the hormone-sensitive lipase binding site on the adipocyte fatty acid-binding protein (AFABP). Identification of the charge quartet on the AFABP/aP2 helix-turn-helix domain. PMID: 18820256
  47. analysis of human, mouse and ovine Hormone Sensitive Lipase PMID: 18824087
  48. PKA activates human HSL against lipid substrates in vitro primarily through phosphorylation of Ser649 and Ser650 PMID: 19018281
  49. These results suggest that the associations between physical activity and body fat and plasma lipoprotein/lipid concentrations in men are dependent on the LIPE C-60G polymorphism,. PMID: 19164092
  50. results suggest that ATGL/CGI-58 acts independently of HSL and precedes its action in the sequential hydrolysis of triglycerides in human hMADS adipocytes PMID: 19433586

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Involvement in disease Lipodystrophy, familial partial, 6 (FPLD6)
Subcellular Location Cell membrane, Membrane, caveola, Cytoplasm, cytosol
Protein Families 'GDXG' lipolytic enzyme family
Database Links

HGNC: 6621

OMIM: 151750

KEGG: hsa:3991

STRING: 9606.ENSP00000244289

UniGene: Hs.656980

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