Human intercellular adhesion molecule 2,ICAM-2 ELISA Kit

Instructions
Code CSB-E07952h
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name intercellular adhesion molecule 2
Alternative Names ICAM2Intercellular adhesion molecule 2 ELISA Kit; ICAM-2 ELISA Kit; CD antigen CD102 ELISA Kit
Abbreviation ICAM2
Uniprot No. P13598
Species Homo sapiens (Human)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 1.25 ng/mL-80 ng/mL
Sensitivity 0.312 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human ICAM-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:5 Average % 107
Range % 100-114
1:10 Average % 103
Range % 101-106
1:20 Average % 87
Range % 84-91
1:40 Average % 94
Range % 90-96
Recovery
The recovery of human ICAM-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 95 90-100
EDTA plasma (n=4) 88 85-91
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
80 2.535 2.633 2.584 2.411
40 2.068 2.102 2.085 1.912
20 1.347 1.330 1.339 1.166
10 0.763 0.732 0.748 0.575
5 0.443 0.454 0.449 0.276
2.5 0.287 0.272 0.280 0.107
1.25 0.215 0.228 0.222 0.049
0 0.176 0.170 0.173  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM2 may play a role in lymphocyte recirculation by blocking LFA-1-dependent cell adhesion. It mediates adhesive interactions important for antigen-specific immune response, NK-cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance.
Gene References into Functions
  1. Lewis-antigen-containing ICAM-2/3 on Jurkat leukemia cells interact with DC-SIGN to regulate DC functions. PMID: 29671117
  2. ICAM2 induction by p53 has a key role in inhibiting cancer cell migration and invasion.ICAM2 acts at least in part through the suppression of the MEK-ERK signaling pathway. PMID: 27556181
  3. INDEED also identified some candidates previously reported to be relevant to HCC, such as intercellular adhesion molecule 2 (ICAM2) and c4b-binding protein alpha chain (C4BPA), which were missed by both Differential expression and differential network analyses PMID: 27592383
  4. Both ICAM-2 and ICAM-1 levels in plasma were markedly increased in Ankylosing Spondylitis Chinese patients compared to controls. PMID: 26641849
  5. Soluble ICAM2 was higher in ADHD patients than in controls. PMID: 26377944
  6. Results indicate that the interaction of ICAM-2 with alpha-actinin is critical to conferring an ICAM-2-mediated non-metastatic phenotype in neuroblastoma cells. PMID: 24704826
  7. with larger patient groups and preferably detailed histopathological and clinical evaluations, are needed to explain the severity of ICAM-1, ICAM-2, and ICAM-3 molecules in Barrett's esophagus PMID: 24474251
  8. ICAM-2 was significantly more pronounced in plasma cell mastitis than in nonpathologic breast tissue. However, no significant differences in ICAM-2 immunoreactivity were detected between ductal epithelium of PCM and non-PCM. PMID: 24457076
  9. Reduced glycosylation of ICAM-2 significantly attenuated, but did not abolish, its ability to suppress metastatic properties of neuroblastoma cells. PMID: 23714211
  10. These findings suggested that the downregulated miR-125b expression was associated with proliferation and radioresistance mechanisms, probably through ICAM2 signalling. PMID: 23591197
  11. Immune surveillance occurs during the early intraepithelial stages of human pancreatic carcinogenesis and is mediated by expression of CXCL17 and ICAM2. PMID: 20955708
  12. Although ICAM-2 colocalizes with moesin and F-actin in microvilli of unstimulated endothelial cells, it is not involved in RhoA activation or cause stress fiber formation upon cross-linking. PMID: 12097408
  13. soluble DC-SIGN bound to gp120-Fc more than 100- and 50-fold better than ICAM-2-Fc and ICAM-3-Fc, respectively. Binding sites are described. PMID: 14970226
  14. The role of ICAM-2 has its recognition as potential therapeutic targets in human diseases. PMID: 15548573
  15. persistently elevated, serum levels of ICAM-2 throughout the various phases and types of Hemorrhagic fever with renal syndrome (HFRS) may be associated with the hyperfunctioning of the cellular immune response. PMID: 16987074
  16. pig and human ICAM-2 promoters exhibit many similarities PMID: 17059578
  17. The binding partner of DC-SIGN on endothelial cells is the glycan epitope Lewis(Y) (Le(Y)), expressed on ICAM-2. PMID: 18155766
  18. Receptor tyrosine kinase EphA2 mediates thrombin-induced upregulation of ICAM-1 in endothelial cells in vitro. PMID: 18768213
  19. ICAM-2 mediates suppression of metastatic phenotype and the interaction of ICAM-2/alpha-actinin/actin represents the first complete membrane-linker protein-actin linkage to impact tumor cell motility in vitro and metastatic potential in an in vivo model. PMID: 18978946

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Subcellular Location Membrane, Single-pass type I membrane protein
Protein Families Immunoglobulin superfamily, ICAM family
Database Links

HGNC: 5345

OMIM: 146630

KEGG: hsa:3384

STRING: 9606.ENSP00000388666

UniGene: Hs.431460

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