Human low density lipoprotein,LDL ELISA Kit

Instructions
Code CSB-E09049h
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name low density lipoprotein,LDL
Alternative Names N/A
Abbreviation LDL
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, urine, saliva
Detection Range 93.75 ng/mL-6000 ng/mL
Sensitivity 23.4 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human LDL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:200 Average % 98
Range % 92-102
1:400 Average % 95
Range % 91-99
1:800 Average % 108
Range % 102-114
1:1600 Average % 85
Range % 80-89
Recovery
The recovery of human LDL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 92 88-96
EDTA plasma (n=4) 104 98-108
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average
6000 0.089 0.091 0.090
3000 0.144 0.148 0.146
1500 0.218 0.222 0.220
750 0.328 0.334 0.331
375 0.488 0.492 0.490
187.5 0.640 0.653 0.647
93.75 0.883 0.913 0.898
0 1.583 1.675 1.629
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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