Human natural resistance-associated macrophage protein 1(NRAMP-1)ELISA kit

Instructions
Code CSB-E17597h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name solute carrier family 11 (proton-coupled divalent metal ion transporters), member 1
Alternative Names LSH ELISA Kit; Natural resistance associated macrophage protein 1 ELISA Kit; Natural resistance-associated macrophage protein 1 ELISA Kit; NRAM1_HUMAN ELISA Kit; NRAMP 1 ELISA Kit; NRAMP ELISA Kit; PBC ELISA Kit; SLC11A1 ELISA Kit; Solute carrier family 11 (proton coupled divalent metal ion transporters) member 1 ELISA Kit; solute carrier family 11 (sodium/phosphate symporters) member 1 ELISA Kit; Solute carrier family 11 member 1 ELISA Kit
Abbreviation SLC11A1
Uniprot No. P49279
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 25 pg/mL-1600 pg/mL
Sensitivity 6.25 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human NRAMP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %85
Range %80-92
1:2Average %87
Range %83-91
1:4Average %95
Range %91-103
1:8Average %90
Range %86-94
Recovery
The recovery of human NRAMP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 10096-104
EDTA plasma (n=4)9690-105
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
16002.471 2.372 2.422 2.293
8001.969 1.906 1.938 1.809
4001.424 1.458 1.441 1.312
2000.982 0.969 0.976 0.847
1000.514 0.553 0.534 0.405
500.247 0.234 0.241 0.112
250.164 0.159 0.162 0.033
00.133 0.125 0.129  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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 Q&A
Q:

kindly enlighten me regarding the the NRAMP! elisa kit, as per the sample types required, shown on this page it is can be assayed in serum or plasma. my question is that do we have it in our serum/plasma in routine?
i am currently working on Genetic susceptibility to tuberculosis in relation to SLC11A1 polymorphism
this gene produces the NRAMP1 protein on human macrophages
so i was intending to quantify this protein in serum / plasma of Tb patients and compare it with levels in serum of controls
so all i want to ask is CAN it be Quantified in Serum samples

A:

Thanks for your kind patience!
#CSB-E17597h,this kit is designed and developed under the reference of https://www.uniprot.org/uniprot/P49279.It is used to test NRAMP-1 protein.If you use TB serum and control serum as corresponding test group and control group,pls take actual meansurement as the real results.

Target Data

Function Divalent transition metal (iron and manganese) transporter involved in iron metabolism and host resistance to certain pathogens. Macrophage-specific membrane transport function. Controls natural resistance to infection with intracellular parasites. Pathogen resistance involves sequestration of Fe(2+) and Mn(2+), cofactors of both prokaryotic and eukaryotic catalases and superoxide dismutases, not only to protect the macrophage against its own generation of reactive oxygen species, but to deny the cations to the pathogen for synthesis of its protective enzymes.
Gene References into Functions
  1. important roles for c-Src tyrosine kinase in phosphorylation and activation of SLC11A1 in macrophages PMID: 29723216
  2. 3'UTR-NRAMP1 gene and VDR-Taq1 gene Polymorphisms are statistically associated with the susceptibility of TB in Andhra Pradesh Population in India. PMID: 28583097
  3. Nramp1 is expressed in microglia and neurons in the striatum of human brain and response for degrading alpha-synuclein oligomers in microglia. PMID: 28476637
  4. This study identified several important polymorphisms in the ITPKC and SLC11A1 genes in Koreans. PMID: 29214786
  5. Studied the role of natural resistance associated macrophage protein 1 (NRAMP1) polymorphisms (D543N, INT4) in pulmonary tuberculosis risk. For D543NA/G polymorphism, no associations were found in all genetic models. For INT4C/G polymorphism, significant increased PTB risk was observed in recessive model. PMID: 28648687
  6. This study shows that genetic variations in the candidate gene SLC11A1 do not affect susceptibility to cutaneous leishmaniasis in the sample population from Pakistan. PMID: 28061874
  7. in patients with non-muscle-invasive bladder cancer, the NRAMP1 D534N polymorphism could increase the risk of recurrence PMID: 29187939
  8. Taken together, this study reveals evidence demonstrating a mechanism by which the LPR6/ GSK3beta/E2F1 axis-upregulated LSH promoted gliomas. PMID: 28042322
  9. Our data suggest that the D543N variant of SLC11A1 gene has a protective effect in the development of rheumatoid arthritis in a Mexican Mestizo population PMID: 28239176
  10. The results indicated that genetic variations of D543N (rs17235409) might be associated with susceptibility to cutaneous leishmaniasis infection. PMID: 27681549
  11. SLC11A1 expression is significantly upregulated in human masticatory mucosa during wound healing PMID: 28005267
  12. We found a statistical association between polymorphisms in 3'UTR region and exon 8 and CL [chi(2) = 13.26; p < 0.05; OR = 17.00; IC of 95% (2.24-128.99)]. Some patients who needed more than 40 doses of Glucantime(R) to heal injuries presented mutations in exons 3, 8, and 15. Multiple or ear lesions were not associated with NRAMP1 polymorphism. PMID: 27830154
  13. distriburtion of polymorphism allelle frequency at the INT4 region associated with oropharyngeal tularemia PMID: 27223255
  14. we conclude that presence of G allele of NRAMP1 in both rs2276631 and rs17235409 location may be a protective factor against chronic periodontitis PMID: 27309481
  15. our meta-analysis demonstrated that no significant association was identified between genetic susceptibility to UC/CD and polymorphisms of NRAMP1 gene, including (GT)n allele 2, 274 C/T, 1729+55del4 (TGTG) +/del in overall population. PMID: 27019053
  16. The ratio of FoxA1 to FoxA2 in lung adenocarcinoma is regulated by LncRNA HOTAIR and chromatin remodeling factor LSH PMID: 26658322
  17. The 274C/T polymorphism in exon 3 and the 469+14G/C polymorphism in intron 4 of the Slc11a1/Nramp1 gene were associated with susceptibility to leprosy, while the allele 2 and 3 of the (GT)n polymorphism in the promoter region were associated with susceptibility and protection to leprosy, respectively. PMID: 26814595
  18. investigation of NRAMP1, MBL, MBL, VDR gene polymorphisms and their interaction with susceptibility to pulmonary tuberculosis in a Chinese population; study suggests that genotypes of many polymorphic genes are associated with TB PMID: 26261060
  19. No significant difference was found for NRAMP1 and hGPX1 gene polymorphisms associated with recurrence time. PMID: 27069153
  20. NRAMP1 3'UTR variants were associated with susceptibility to M. tuberculosis infection. PMID: 26578819
  21. genetic association studies in population in Mexico: Data suggest an SNP in SLC11A1 (D543N, G/A genotype) is associated with treatment failure (after treatment with combinations of antitubercular agents) in male patients with pulmonary tuberculosis. PMID: 26353180
  22. Genetic variants of SLC11A1 are associated with both autoimmune and infectious diseases. PMID: 25856512
  23. SLC11A1 gene polymorphisms might have a relevant role in the pathology of leishmaniasis, directing towards susceptibility outcome of this disease PMID: 25603101
  24. Our data provide insights into the possible role of SLC11A1 variation in visceral leishmaniasis susceptibility. PMID: 25151047
  25. Nramp1 expression is up-regulated by cytokines, and its function helps to produce nitric oxide along with other pro-inflammatory responses. [Review] PMID: 26055722
  26. Study results have demonstrated, that of all investigated polymorphic variants of genes IL4 (C-590T), IL4RA (150V), TNF (G-308A) and SLC11A1 (D543N) in patients with chronic liver diseases of various etiology PMID: 26281177
  27. Highly statistically significant associations were detected between IVS4+44 C/A polymorphism in the DMT1 gene and iron and lead levels (p=0.001 and p=0.036, respectively), but no association was found with cadmium level (p=0.344). PMID: 25483413
  28. Polymorphism of the NRAMP1 gene was investigated by PCR amplification followed by RFLP analysis... patients with heterozygosity of intron 4 (GC) and/or maternal infection with helminth parasites showed reduced efficacy of BCG vaccine against tuberculosis PMID: 23408448
  29. Polymorphisms in the NRAMP1 gene, VDR gene, HLA-DRB1 gene, and HLA-DQB1 gene are statistically associated with susceptibility to TB in the Chinese Kazakh population. PMID: 24024195
  30. Study identifies SLC11A1 as a novel candidate for OM susceptibility, particularly in children with adenoids intact. PMID: 23538334
  31. Transcription factor ATF-3 regulates allele variation phenotypes of the human SLC11A1 gene. PMID: 23187734
  32. Polymorphisms in SLC11A1 were probably associated with the development of drug-resistant TB in Chinese Han population. PMID: 21457666
  33. Our findings reveal an unsuspected role for SLC11A1 in determining Clostridium. difficile pathogenicity PMID: 23690404
  34. These results suggest that the Sardinian population might be prone to develop autoimmune disease due to polymorphisms in immunomodulating the SLC11A1 gene. PMID: 23492997
  35. In the absence of LSH, the histone variant H2AX is not efficiently phosphorylated in response to DNA damage. PMID: 22946062
  36. SLC11A1-expressing lymphocytes are more prone to activation and retention of tyrosine phosphorylation, consistent with a role for inhibition of protein tyrosine phosphatases. PMID: 23509347
  37. The bactericidal activity of monocytes in patients with Reactive Arthritis is lower than that in healthy controls. The SLC11A1 274C/T and 823C/T polymorphisms may be associated with the decreased bactericidal activity of the monocytes. PMID: 23244274
  38. The T allele of rs2279014 in the 3' untranslated region of SLC11A1 was associated with protection from MAC disease when comparing allele frequencies with an odds ratio of 0.582. PMID: 22387151
  39. findings of the present study do not support the hypothesis that Nramp1...might play a role in influencing the growth of bacilli and progression of cavitary tuberculosis rather than susceptibility to M. tuberculosis infection. PMID: 23137204
  40. results suggest an association of NRAMP1 3'-UTR and D543N polymorphisms with susceptibility to mycobacterial infection in Tunisian populations in relation to age and sex PMID: 22609013
  41. This meta-analysis suggests that polymorphisms in the SLC11A1 gene contribute to TB (both pulmonary TB and extra-pulmonary TB), particularly in Asians PMID: 22326178
  42. The results shed light on the role of NRAMP1 in susceptibility to tuberculosis disease and provide a plausible explanation for NRAMP1 and MBL genetic heterogeneity in tuberculosis susceptibility. PMID: 21554230
  43. The association between sarcoidosis and four polymorphisms of the SLC11A1 gene, was investigated. PMID: 22160516
  44. Report a significant association between SLC11A1 gene variants and pulmonary tuberculosis with respect to susceptibility and subsequent disease progression in East India. PMID: 22008758
  45. Homology implies that inverted structural symmetry facilitates Slc11 H(+)-driven Me(2+) import and provides a 3D framework to test structure-activity relationships in macrophages and study functional evolution of MntH/Nramp (Slc11) carriers. PMID: 21948377
  46. No associations were observed between visceral leishmaniasis and genetic polymorphisms of SLC11A1 that were either robust to correction for multiple testing or replicated across primary and replication samples. PMID: 21599885
  47. conclude that rs3731685 (INT4) in the SLC11A1 gene may be associated with T1D susceptibility in the European ancestry population studied PMID: 21524304
  48. The 3'UTR polymorphism of NRAMP1 showed significant correlation with susceptibility to tuberculosis infection. PMID: 21710855
  49. association between SLC11A1 polymorphisms and tuberculosis susceptibility analysed by meta-analysis PMID: 21283567
  50. recruitment of the SWI/SNF complex initiated Z-DNA formation and subsequently helped to transactivate the SLC11A1 gene. PMID: 21300803

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Subcellular Location Membrane, Multi-pass membrane protein
Protein Families NRAMP family
Tissue Specificity Macrophages; peripheral blood leukocytes, lung, spleen and liver.
Database Links

HGNC: 10907

OMIM: 600266

KEGG: hsa:6556

STRING: 9606.ENSP00000233202

UniGene: Hs.591607

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