Code | CSB-E08787h |
Size | 96T,5×96T,10×96T |
Price | Request a Quote or Start an on-line Chat |
Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
To assess the linearity of the assay, samples were spiked with high concentrations of human NF-κB p65 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:1 | Average % | 88 | ||||
Range % | 83-92 | |||||
1:2 | Average % | 101 | ||||
Range % | 95-104 | |||||
1:4 | Average % | 102 | ||||
Range % | 98-105 | |||||
1:8 | Average % | 95 | ||||
Range % | 90-98 |
The recovery of human NF-κB p65 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 93 | 89-96 | ||||
EDTA plasma (n=4) | 95 | 91-98 | ||||
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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This human NF-κB p65 ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of human NF-κB p65 in different samples, including serum, plasma, or tissue homogenates. The enzyme-substrate chromogenic reaction is also used to amplify the signal and quantify the levels of the analyte through the intensity of the colored product. The color intensity positively correlates with the amount of NF-κB p65 bound in the initial step.
p65, also known as RELA, is one of the five components that form the NF-κB transcription factor family and plays a central role in inflammation and immunity. p65 dimerizes with NF-κB1 (p50) to form the heterodimer NF-κB, which is involved in multiple physiological and pathological gene regulation processes, such as inflammatory response, immune response, cell division, proliferation, and apoptosis. Studies have shown that the expression of NF-κB P65 in liver cancer tissues is higher than that in adjacent tissues, and the key to regulating the function of NF-κB is to inhibit the promoting effect of P65 on liver tumors. It is speculated that blocking the activity of NF-κB P65 can inhibit the differentiation and growth of tumor cells, providing a new idea for clinical treatment.
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I am interested in the nfkb p 65 ELISA kit.
However, I am little confused about the kit as nfkb is an intracellular transcription factor. It's found in the cytoplasm and when activated, it translocates to the nucleus.
So I want to know what is the indication of measuring this factor in the plasma or serum?
What will this express? What is the difference between nfkb kit and nfkb p65 kit?
The CSB-E08787h kit is designed by taking the reference from https://www.uniprot.org/uniprot/Q04206, which is for p65.
As for intracellular proteins, it is generally not secreted outside the cell, but in some metabolism or pathology, cell aging enrichment can be released to the blood, and it can be detected at this time.
CSB-E12107h is designed for p105 by taking the reference from https://www.uniprot.org/uniprot/P19838
NFkb p65 ELISA Kit and NF-κB ELISA Kit target different proteins, detect different objects and use different antibodies.