Human receptor-binding cancer antigen expressed on SiSo cells,RCAS1 ELISA kit

Code CSB-E12153h
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. O00559
Lead Time 3-5 working days
Abbreviation EBAG9
Protein Biological Process 1 Apoptosis/Autophagy
Target Name estrogen receptor binding site associated, antigen, 9
Alias EB9, PDAF, RCAS1, cancer associated surface antigen|estrogen receptor binding site associated antigen 9
Species Homo sapiens (Human)
Protein Biological Process 3 Apoptosis
Sample Types serum, tissue homogenates
Detection Range 0.78 U/mL-50 U/mL
Sensitivity 0.195 U/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This gene was identified as an estrogen-responsive gene. Regulation of transcription by estrogen is mediated by estrogen receptor which binds to the estrogen-responsive element (ERE) found in the 5 -flanking region of this gene. The encoded protein is a tumor-associated antigen that is expressed at high frequency in a variety of cancers. Two transcript variants differing in the 5 UTR, but encoding the same protein, have been identified for this gene.
HGNC 3123
RGD 1307293
MGI 1859920
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human RCAS1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %94
Range %90-98
1:2Average %96
Range %91-101
1:4Average %100
Range %95-106
1:8Average %105
Range %99-111
Recovery
The recovery of human RCAS1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9692-102
EDTA plasma (n=4)10095-105
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
U/mlOD1OD2AverageCorrected
502.702 2.758 2.730 2.577
252.211 2.219 2.215 2.062
12.51.566 1.596 1.581 1.428
6.250.984 0.997 0.991 0.838
3.120.562 0.581 0.572 0.419
1.560.439 0.407 0.423 0.270
0.780.291 0.281 0.286 0.133
00.151 0.154 0.153  
References
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Function May participate in suppression of cell proliferation and induces apoptotic cell death through activation of interleukin-1-beta converting enzyme (ICE)-like proteases.
Subcellular Location Golgi apparatus membrane, Single-pass type III membrane protein
Tissue Specificity Widely expressed. Expressed in ovary, testis, prostate, thymus, muscle and heart, but not in small intestine, colon, lymph nodes, or peripherical blood lymphocytes. The protein is not detected in any of the above organs.
Database Links

HGNC: 3123

OMIM: 605772

KEGG: hsa:9166

STRING: 9606.ENSP00000337675

UniGene: Hs.409368

Pathway Estrogen signaling pathway

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