Human receptor-binding cancer antigen expressed on SiSo cells,RCAS1 ELISA kit

Instructions
Code CSB-E12153h
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application

Product Details

Target Name estrogen receptor binding site associated, antigen, 9
Alternative Names EB9, PDAF, RCAS1, cancer associated surface antigen|estrogen receptor binding site associated antigen 9
Abbreviation EBAG9
Uniprot No. O00559
Species Homo sapiens (Human)
Sample Types serum, tissue homogenates
Detection Range 0.78 U/mL-50 U/mL
Sensitivity 0.195 U/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human RCAS1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %94
Range %90-98
1:2Average %96
Range %91-101
1:4Average %100
Range %95-106
1:8Average %105
Range %99-111
Recovery
The recovery of human RCAS1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9692-102
EDTA plasma (n=4)10095-105
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
U/mlOD1OD2AverageCorrected
502.702 2.758 2.730 2.577
252.211 2.219 2.215 2.062
12.51.566 1.596 1.581 1.428
6.250.984 0.997 0.991 0.838
3.120.562 0.581 0.572 0.419
1.560.439 0.407 0.423 0.270
0.780.291 0.281 0.286 0.133
00.151 0.154 0.153  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days
Still Have Questions? Leave a Message or Start an on-line Chat

Target Data

Function May participate in suppression of cell proliferation and induces apoptotic cell death through activation of interleukin-1-beta converting enzyme (ICE)-like proteases.
Subcellular Location Golgi apparatus membrane, Single-pass type III membrane protein
Tissue Specificity Widely expressed. Expressed in ovary, testis, prostate, thymus, muscle and heart, but not in small intestine, colon, lymph nodes, or peripherical blood lymphocytes. The protein is not detected in any of the above organs.
Database Links

HGNC: 3123

OMIM: 605772

KEGG: hsa:9166

STRING: 9606.ENSP00000337675

UniGene: Hs.409368

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