Human regenerating gene Ⅳ,REG-4 ELISA Kit

Code CSB-E11890h
Size 96T,5×96T,10×96T
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Product Details

Target Name
regenerating islet-derived family, member 4
Alternative Names
Gastrointestinal secretory protein ELISA Kit; GISP ELISA Kit; Reg IV ELISA Kit; REG like protein ELISA Kit; REG-4 ELISA Kit; REG-like protein ELISA Kit; Reg4 ELISA Kit; REG4_HUMAN ELISA Kit; Regenerating gene type IV ELISA Kit; Regenerating islet derived family member 4 ELISA Kit; Regenerating islet derived protein 4 precursor ELISA Kit; Regenerating islet-derived protein 4 ELISA Kit; Regenerating islet-derived protein IV ELISA Kit; REGIV ELISA Kit; RELP ELISA Kit
Abbreviation
REG4
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.312 ng/mL-20 ng/mL
Sensitivity
0.078 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human REG-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 87  
Range % 83-92  
1:2 Average % 92  
Range % 90-95  
1:4 Average % 96  
Range % 93-99  
1:8 Average % 95  
Range % 88-98  
Recovery
The recovery of human REG-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 91 89-94  
EDTA plasma (n=4) 92 90-94  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
20 2.832 2.801 2.817 2.676  
10 2.238 2.187 2.213 2.072  
5 1.419 1.385 1.402 1.261  
2.5 0.673 0.654 0.664 0.523  
1.25 0.434 0.411 0.423 0.282  
0.625 0.281 0.269 0.275 0.134  
0.312 0.211 0.202 0.207 0.066  
0 0.143 0.139 0.141    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human REG4 ELISA Kit was designed for the quantitative measurement of Human REG4 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.

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Target Background

Function
(From Uniprot)
Calcium-independent lectin displaying mannose-binding specificity and able to maintain carbohydrate recognition activity in an acidic environment. May be involved in inflammatory and metaplastic responses of the gastrointestinal epithelium.
Gene References into Functions
  1. Our study demonstrated that Reg IV positively regulates the expression of SOX9 and is involved in tumor cell invasion and migration in gastric cancer. PMID: 29587675
  2. Reg4 inhibited apoptosis through regulating MAPK/Erk/Bim signaling pathway and thereby enhanced the resistance of gastric cancer to 5-Fluorouracil. PMID: 28759561
  3. Data indicate that REG4 tissue expression is a prognostic marker in subgroups of pancreatic ductal adenocarcinoma patients, and Serum REG4 level might be useful in differential diagnosis between pancreatic ductal adenocarcinoma and chronic pancreatitis. PMID: 29542402
  4. REG4 promotes peritoneal metastasis of gastric cancer through GPR37 and triggers a positive feedback loop. PMID: 27036049
  5. Study demonstrated that REG4, which is overexpressed in pancreatic ductal adenocarcinoma and secreted by cancer cells, promoted macrophage polarization to M2, through at least in part, activation of ERK1/2 and CREB. PMID: 26531138
  6. REG4 is a transcriptional target of GATA6 and is essential for colorectal tumorigenesis. PMID: 26387746
  7. This study identifies REG4 as a potential serum biomarker for histotype-specific detection of mucinous ovarian cancer and suggests serum REG4 measurement as a non-invasive diagnostic tool for follow-up of patients with mucinous ovarian cancer. PMID: 26981633
  8. aberrant REG4 expression plays an essential role in early ovarian carcinogenesis and is closely linked to mucinous ovarian tumors, differentiation and adverse prognosis of ovarian cancer by modulating proliferation, apoptosis, migration and invasion. PMID: 26077911
  9. REG4 expression associated with favorable clinicopathological parameters and with higher overall survival from non-mucinous PMID: 25295732
  10. The Reg IV may be involved in the finetuning of functions exerted by the neuroendocrine cells in the GI-tract. PMID: 23499801
  11. Colorectal tumor patients with Reg4- and MMP-7-positive tumors had extremely poor overall survival. PMID: 25338725
  12. High expression of REG4 is associated with poor therapeutic response, adverse outcome and an aggressive phenotype in rectal cancer patients treated with neoadjuvant chemoradiotherapy. PMID: 25155043
  13. miR-24 functions as a novel tumor suppressor in gastric cancer, and the anti-oncogenic activity may involve its inhibition of the target gene REG4. PMID: 24886316
  14. REG4 expression is common in mucinous borderline ovarian tumors of the intestinal type as it is absent in the endocervical-like form tumors. PMID: 23958547
  15. the combination of VEGF-C and Reg IV may be a promising factor for clinical staging to supplement the classical TNM classification system. PMID: 24101199
  16. the stemness properties of control mammospheres and RegIV knockdown mammospheres were compared by tumourigenicity assay in vivo and plate colony formation assay in vitro. PMID: 24064664
  17. Reg4-induced mitogenesis involves Akt-GSK3beta-beta-Catenin-TCF-4 signaling in human colorectal cancer. PMID: 24151146
  18. Immunohistochemistry against known cell-type markers on serial sections has localised the expression of REGs to metaplastic Paneth cells (REG1A, REG1B and REG3A) and enteroendocrine cells (REG4), with a marked expansion of expression during inflammation. PMID: 23519454
  19. CDX2 protein directly regulates Reg IV expression in gastric cancer PMID: 23133598
  20. RegIV may play an important role in the intrinsic resistance of gastric cancer cells to 5-FU. PMID: 23010741
  21. Suggest that Reg IV might accelerate disease progression and act as a candidate prognostic marker for gliomas. PMID: 22713481
  22. REG4 promotes not only growth but also in vitro invasiveness of pancreatic cancer cells by upregulating MMP-7 and MMP-9. PMID: 22957785
  23. TGF-beta signalling reduces the expression of ALDH1 and REG4, and the size of the ALDH1+ cell population. PMID: 22430847
  24. Knockdown of Reg IV impacted the ability of insulin and EGF to stimulate downstream tyrosine phosphorylation. PMID: 21445968
  25. REG4, BIRC5 and NEIL2 genes might have a role in the sensitivity of cancer patients to radiotherapy PMID: 22199273
  26. Overexpression of REGIV mRNA is assessed with peritoneal recurrence in gastric adenocarcinoma. PMID: 21780125
  27. REG4 may be a prognostic indicator and a better serum marker than carcinoembryonic antigen and carbohydrate antigen 19-9 for early diagnosis of gastric cancer. PMID: 21419474
  28. GLI1 promotes RegIV transcription by binding to the RegIV gene promoter in pancreatic cancer. PMID: 21494603
  29. The solution structure of hRegIV-P91S was determined, showing that it adopts a typical fold of C-type lectin. Based on the chemical shift perturbations of amide resonances, two calcium-independent mannan-binding sites were proposed PMID: 20692269
  30. REG4 may play an important role in the development and progression of colorectal cancer, as well as in intestinal morphogenesis and epithelium restitution PMID: 20126989
  31. RegIV is expressed in the gastrointestinal tract and in digestive tract neuroendocrine tumor PMID: 20349522
  32. REG IV overexpression may be an early event in colorectal carcinoma carcinogenesis. PMID: 20183800
  33. RegIV enhances LoVo cell migration and invasion, and its CRD domain is critical for these effects. PMID: 20417867
  34. REG4 is expressed in pancreatic cancer, and serum levels of REG4 offer a useful indicator for distinguishing between patients with pancreatic cancer and healthy subjects PMID: 19789838
  35. Reg IV as an important modulator of gastrointestinal cell susceptibility to irradiation; hence, it is a potential target for adjunctive treatments for human colorectal cancer and other gastrointestinal malignancies. PMID: 19900450
  36. reg4 is amplified in the early stages of pancreatic cancer development PMID: 19834624
  37. Data demonstrated in vitro and in vivo that REG4 protein overexpression was associated with an unfavorable response to preoperative chemoradiotherapy. PMID: 19546835
  38. Local Reg IV expression may be influenced by the growth factors basic fibroblast growth factor and somatotropin and dtheri receptors PMID: 19924642
  39. Results suggest that RELP might be involved in inflammatory and metaplastic responses of the gastrointestinal epithelium. PMID: 12819006
  40. overexpression of Reg IV may be an early event in colorectal carcinogenesis. PMID: 14550954
  41. Increased expression of Reg IV is associated wirh hormone refractory metastatic prostate cancer PMID: 15788672
  42. RELP serves as a marker for appendiceal mucinous cystadenomas and pseudomyxoma peritonei and may contribute to the pathogenesis of these disorders. PMID: 16323007
  43. Reg IV is a potent activator of the EGF receptor/Akt/AP-1 signaling pathway in colorectal carcinoma PMID: 16401477
  44. Overexpression of REG4 is associated with pancreatic cancer PMID: 16918991
  45. serum Reg IV concentration may predict metastatic recurrence of colorectal cancer in the liver.Reg IV protein expression induced by growth factors may function as a growth-promoting and/or an antiapoptotic factor in the peritumoral mucosa of CRC. PMID: 18187959
  46. RegIV expression was markedly higher in gastric cancer patients with peritoneal metastases compared to those without; level of RegIV mRNA in gastric cancer patients was related to the extent of wall penetration PMID: 18505053
  47. Reg IV staining can aid in diagnosis of gastrointestinal signet ring cell carcinoma. PMID: 18580680
  48. These results suggest that Reg IV expression is an independent prognostic indicator of relapse after radical prostatectomy. PMID: 18754868
  49. Reg IV might accelerate cell growth and disease progression of adenoid cystic carcinomas . PMID: 19076683
  50. among major urologic cancers, Reg IV is expressed frequently in prostate cancer, and that serum Reg IV represents a novel biomarker for prostate cancer PMID: 19082448

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Subcellular Location
Secreted.
Tissue Specificity
Highly expressed in the gastrointestinal tract including the duodenum, jejunum, ileum, ileocecum, appendix, descending colon, pancreas and small intestine. Weakly expressed in normal colon and stomach. Strongly expressed in most colorectal tumors than in
Database Links

HGNC: 22977

OMIM: 609846

KEGG: hsa:83998

STRING: 9606.ENSP00000256585

UniGene: Hs.660883

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