Human ribonuclease pancreatic (RNASE1/RIB1/RNS1) ELISA kit

Instructions
Code CSB-E09498h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name ribonuclease, RNase A family, 1 (pancreatic)
Alternative Names HP-RNase ELISA Kit; Pancreatic ribonuclease ELISA Kit; Rib 1 ELISA Kit; RIB-1 ELISA Kit; Rib1 ELISA Kit; Ribonuclease 1 ELISA Kit; Ribonuclease A ELISA Kit; Ribonuclease a family 1 ELISA Kit; Ribonuclease pancreatic ELISA Kit; Ribonuclease RNase A family 1 ELISA Kit; Ribonuclease RNase A family 1 pancreatic ELISA Kit; RNAS1_HUMAN ELISA Kit; RNase 1 ELISA Kit; RNase A ELISA Kit; RNase UpI-1 ELISA Kit; Rnase1 ELISA Kit; RNAseA ELISA Kit
Abbreviation RNASE1
Uniprot No. P07998
Species Homo sapiens (Human)
Sample Types serum, plasma, cell lysates
Detection Range 0.312 ng/mL-20 ng/mL
Sensitivity 0.078 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Epigenetics and Nuclear Signaling
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of Human RNASE1/RIB1/RNS1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:20Average %102
Range %98-108
1:40Average %89
Range %85-93
1:80Average %95
Range %90-99
1:160Average %102
Range %97-105
Recovery
The recovery of Human RNASE1/RIB1/RNS1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9187-95
EDTA plasma (n=4)8985-93
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
202.815 2.719 2.767 2.680
102.314 2.215 2.265 2.178
51.641 1.542 1.592 1.505
2.50.834 0.813 0.824 0.737
1.250.476 0.457 0.467 0.380
0.6250.294 0.282 0.288 0.201
0.3120.182 0.172 0.177 0.090
00.088 0.086 0.087
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function Endonuclease that catalyzes the cleavage of RNA on the 3' side of pyrimidine nucleotides. Acts on single-stranded and double-stranded RNA.
Gene References into Functions
  1. Data suggest that ribonuclease inhibitor (RNH1) protects HeLa cells from ribonuclease 1 (RNase 1). PMID: 27806571
  2. Focusing on the increase in an N-glycosylated Asn residue of serum pancreatic ribonuclease 1, specifically Asn(88), affords a new diagnostic marker for pancreatic cancer PMID: 25336120
  3. Deletion of five residues in the hinge loop of pancreatic ribonuclease induces formation of a domain-swapped dimer that leads to the generation of linear aggregates of pancreatic RNase, revealed by the crystal packing. PMID: 24100329
  4. The reduced conformational flexibility of eosinophil cationic protein can be dynamically and functionally reproduced in the RNase A scaffold. PMID: 23135272
  5. The nuclear transport of PE5 is critical for its cytotoxicity. PMID: 20352290
  6. The role on the dimerization process of different residues of a domain-swapped dimer human pancreatic ribonuclease variant. PMID: 21767499
  7. In human pancreatic ribonuclease both glutamine 28 and arginine 39 are important for the cleavage of dsRNA. PMID: 21408145
  8. Vascular RNase1 and RNase5 are mainly produced by vascular endothelial cells and can serve, depending on the vascular bed, different functions in vascular homeostasis and endothelial cell responses. PMID: 21103661
  9. Studies illustrate of the making dimeric pancreatic RNase through removal by directed mutagenesis of most of the N-terminal alpha-helix to the remainder of the protein. PMID: 19156888
  10. Human endothelial cells selectively express large amounts of pancreatic-type ribonuclease (RNase 1) PMID: 12210760
  11. Results show that Glycine 38 is crucial for the full catalytic activity of the human enzyme on duplex RNA as its substitution with aspartate or alanine results in a drastic reduction in the dsRNA cleavage activity of HPR. PMID: 12237131
  12. Results reveal the dendritic cell-activating activity of pancreatic ribonuclease and suggest that it is a likely participant of inflammatory and immune responses--an endogenous multifunctional immune alarmin. PMID: 15528350
  13. Altogether the results suggest that the pressure-folding transition state of ribonuclease A looks like a collapsed globule with some secondary structure and a weakened hydrophobic core. This is the first direct comparison using a set of mutants. PMID: 16597833
  14. RNase-1 has ribonuclease H activity. PMID: 16738129
  15. Human pancreatic-ribonuclease interacts with importin alpha through different basic residues, including Lys1 and the arginine clusters 31-33 and 89-91. PMID: 16780873
  16. The results were confirmed at the level of mRNA and protein, and suggested that four genes (OPCML, RNASE1, YES1 and ACK1) could play a key role in the tumorigenesis and metastasis of gastric cancer. PMID: 17109515
  17. Structural and energetic aspects of the interaction between human RI (hRI) and human pancreatic ribonuclease (RNase 1), is reported. PMID: 17350650
  18. Coulombic forces mediate extracellular and intracellular equilibria in a dichotomous manner that both endangers cells and defends them from the potentially lethal enzymatic activity of ribonucleases. PMID: 17705507
  19. irrespective of differences in ethnic groups, RNASE1 might show markedly low heterogeneity in its genetic structure with regard to these SNPs PMID: 18219569
  20. Multiple side chain conformations observed for key surface residues are proposed to be crucial for membrane binding as well as translocation and efficient RNA hydrolysis. PMID: 18495155

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Subcellular Location Secreted
Protein Families Pancreatic ribonuclease family
Tissue Specificity Pancreas and other tissues and body fluids (indicating it may have other physiological functions besides its role in digestion).
Database Links

HGNC: 10044

OMIM: 180440

KEGG: hsa:6035

STRING: 9606.ENSP00000344193

UniGene: Hs.78224

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