Monkey Interferon γ ,IFN-γ ELISA Kit

Instructions
Code CSB-E10049Mo
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name interferon, gamma
Alternative Names IFNG ELISA Kit; Interferon gamma ELISA Kit; IFN-gamma ELISA Kit
Abbreviation IFNG
Uniprot No. P63310
Species Monkey
Sample Types serum, plasma, tissue homogenates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.8 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
20001.830 1.990 1.910 1.805
10001.496 1.398 1.447 1.342
5001.133 1.138 1.136 1.031
2500.932 0.902 0.917 0.812
1250.544 0.535 0.540 0.435
62.50.212 0.226 0.219 0.114
31.250.117 0.111 0.114 0.009
00.106 0.104 0.105  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
Gene References into Functions
  1. IFNgamma expression in colonic epithelial cells was regulated by TGFB1. PMID: 25769244
  2. Higher KIR2DL4 copy numbers is associated with an increased IFN-gamma production in NK cell subsets in SIV-infected Mamu-A*01-negative rhesus macaques. PMID: 23449795
  3. Data show that viral set-point in simian immunodeficiency virus diseasewas is associated with expression of interferon gamma -stimulated genes. PMID: 17719201
  4. Low Sociable animals showed alterations in lymph node expression of the immunoregulatory cytokine interferon-gamma. PMID: 18068331
Subcellular Location Secreted
Protein Families Type II (or gamma) interferon family
Tissue Specificity Released primarily from activated T lymphocytes.
Database Links

KEGG: mcc:574282

STRING: 9544.ENSMMUP00000025266

UniGene: Mmu.3373

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