Monkey interleukin 16 (lymphocyte chemoattractant factor) (IL16) ELISA kit

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Code CSB-EL011596RH
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. O62675
Lead Time 5-7 working days
Protein Biological Process 2 chemokine
Abbreviation IL16
Protein Biological Process 1 Immunity
Target Name interleukin 16 (lymphocyte chemoattractant factor)
Alias FLJ16806, FLJ42735, FLJ44234, HsT19289, IL-16, LCF, prIL-16, OTTHUMP00000190849|OTTHUMP00000190850|interleukin 16|lymphocyte chemoattractant factor
Species Monkey
Protein Biological Process 3 Chemotaxis
Sample Types serum, plasma, tissue homogenates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.81 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This protein is a pleiotropic cytokine that functions as a chemoattractant, a modulator of T cell activation, and an inhibitor of HIV replication. The signaling process of this cytokine is mediated by CD4. The product of this gene undergoes proteolytic processing, which is found to yield two functional proteins. The cytokine function is exclusively attributed to the secreted C-terminal peptide, while the N-terminal product may play a role in cell cycle control. Caspase 3 is reported to be involved in the proteolytic processing of this protein. Two transcript variants encoding different isoforms have been found for this gene.
HGNC 5980
RGD 1583872
MGI 1270855
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
2000 2.658 2.574 2.616 2.531
1000 2.166 2.323 2.245 2.160
500 1.572 1.599 1.586 1.501
250 0.725 0.784 0.755 0.670
125 0.344 0.354 0.349 0.264
62.5 0.256 0.266 0.261 0.176
31.25 0.142 0.151 0.147 0.062
0 0.083 0.087 0.085  
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Function Interleukin-16 stimulates a migratory response in CD4+ lymphocytes, monocytes, and eosinophils. Primes CD4+ T-cells for IL-2 and IL-15 responsiveness. Also induces T-lymphocyte expression of interleukin 2 receptor. Ligand for CD4 (By similarity).
Subcellular Location Interleukin-16: Secreted, SUBCELLULAR LOCATION: Pro-interleukin-16: Cytoplasm, Nucleus
Database Links

KEGG: mcc:574100

STRING: 9544.ENSMMUP00000015164

UniGene: Mmu.2764

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