Mouse β-Thromboglobulin,β-TG ELISA Kit

Instructions
Code CSB-E07888m
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name β-Thromboglobulin,β-TG
Abbreviation β-TG
Species Mus musculus (Mouse)
Sample Types serum, plasma
Detection Range 0.312 ng/mL-20 ng/mL
Sensitivity 0.078 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse β-TG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:1 Average % 86    
Range % 81-90    
1:2 Average % 94    
Range % 90-99    
1:4 Average % 97    
Range % 92-101    
1:8 Average % 105    
Range % 100-110    
Recovery
The recovery of mouse β-TG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 95 90-100    
EDTA plasma (n=4) 88 84-92    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
ng/ml OD1 OD2 Average Corrected    
20 2.388 2.295 2.342 2.133    
10 1.739 1.658 1.699 1.490    
5 1.026 1.002 1.014 0.805    
2.5 0.600 0.581 0.591 0.382    
1.25 0.395 0.407 0.401 0.192    
0.625 0.321 0.339 0.330 0.121    
0.312 0.261 0.273 0.267 0.058    
0 0.213 0.204 0.209      
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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