Mouse γ-Secretase ELISA kit

Code CSB-E12142m
Size 96T,5×96T,10×96T How to order?
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Mouse APH1B ELISA Kit was designed for the quantitative measurement of Mouse APH1B protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 7.8 pg/mL-500 pg/mL and the sensitivity is 1.95 pg/mL.

Target Name anterior pharynx defective 1 homolog B (C. elegans)
Alternative Names Aph1b; Gamma-secretase subunit APH-1B; APH-1b; Aph-1beta
Abbreviation APH1B
Uniprot No. Q8C7N7
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 7.8 pg/mL-500 pg/mL
Sensitivity 1.95 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Neuroscience
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse γ-Secretase in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %96
Range %90-102
1:2Average %102
Range %97-107
1:4Average %89
Range %87-93
1:8Average %94
Range %90-98
Recovery
The recovery of mouse γ-Secretase spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 10399-106
EDTA plasma (n=4)9589-102
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
5002.234 2.356 2.295 2.130
2501.367 1.378 1.373 1.208
1250.810 0.824 0.817 0.652
62.50.492 0.452 0.472 0.307
31.20.313 0.306 0.310 0.145
15.60.236 0.250 0.243 0.078
7.80.205 0.209 0.207 0.042
00.160 0.169 0.165
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Probable subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral proteins such as Notch receptors and APP (amyloid-beta precursor protein). It probably represents a stabilizing cofactor for the presenilin homodimer that promotes the formation of a stable complex. Probably present in a minority of gamma-secretase complexes compared to APH1A.
Gene References into Functions
  1. Loss of Aph1B is associated with memory deficit. PMID: 26942869
  2. Prostaglandin I2 accumulation in neuronal cells activates PKA/CREB and JNK/c-Jun signaling pathways by phosphorylation, which results in APH-1alpha/1beta expression. PMID: 27240539
  3. Abeta oligomers in the cerebrospinal fluid (CSF) further promoted the expression of APH-1alpha/-1beta (by >2.5-fold), which enhances the gamma-cleavage of APP and Abeta deposition during AD progression PMID: 26293690
  4. analysis of hippocampal circuitry via Aph1b-gamma-secretase/neuregulin 1 signalling PMID: 24891237
  5. Upregulation of PS1/gamma-secretase activity may be a risk factor for late onset sporadic Alzheimer's disease. PMID: 22140537
  6. study shows gamma-secretase complexes containing different Aph1 subunits have heterogeneous properties; inactivation of Aph1B gamma-secretase in an Alzheimer disease model led to improvement of disease-relevant features without Notch-related side effects PMID: 19299585

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Subcellular Location Membrane; Multi-pass membrane protein.
Protein Families APH-1 family
Database Links

KEGG: mmu:208117

STRING: 10090.ENSMUSP00000034934

UniGene: Mm.41291

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