Mouse 1,25-dihydroxyvitamin D3 (DVD/DHVD3) ELISA kit

Instructions
Code CSB-E13697m
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name 1,25-dihydroxyvitamin D3
Abbreviation 1,25 DHVD3
Species Mus musculus (Mouse)
Sample Types serum
Detection Range 1000 fmol/L-5000 fmol/L
Sensitivity 250 fmol/L
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Competitive
Precision

Intra-assay Precision (Precision within an assay): CV%<10%

 

Three samples of known concentration were tested twenty times on one plate to assess.

 

Inter-assay Precision (Precision between assays): CV%<15%

 

Three samples of known concentration were tested in twenty assays to assess.

 

 

 

Linearity

 To assess the linearity of the assay, samples were spiked with high concentrations of Mouse1,25-dihydroxyvitamin D3 (1.25 DVD/DHVD3) in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Typical Data

 These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Q&A and Customer Reviews

 Q&A
Q:

Please let us know the sulfuric acid content of [stop solution] .

A:
Thanks for your inquiry.The sulfuric acid content of [stop solution] is 4N.Pls let me know if you have any further questions. Thank you.
Q:

Can you please provide the following information about this kit:
a. Capture antibody or capture antigen – immunogen type, sequence (including species source and accession no. if available),expression host, clonality and host species.
b. Detection antibody (if applicable) - immunogen type, sequence (including species source and accession no. if available),expression host, clonality and host species.
c. Standard – if recombinant protein please specify sequence (including species source and accession no. if available) and expression host.
e. Please send the manual
f. Please send us also, if possible, your in-house data for plasma samples for customer review.

A:
Thanks for your inquiry.
CSB-E13697m
This is a competitive kit and there is only one antibody in the kit.
The plate is coated with rat monoclonal antibody.
The standard is purified 1,25 DHVD3.
I will send you the manual by e-mail.
This kit can only test serum samples. We tested serum samples before and the test value is 2000-4000fmol/L under 1:40 dilution. The final concentration is 80--160pmol/L. The manual will be sent to you by e-mail.
Pls let me know if you have any further questions. Thank you.
Q:

I am currently evaluating different 1,25-dihydroxyvitamin D3 ELISA Kits from various suppliers. Could you therefore please send me the assay protocol of your kit, in order that I can get an idea as to how your assay works? I noticed that many suppliers require an extraction step in organic solvents, but it is not clear to me whether the same is the case for your product.

A:
Thanks for your inquiry.
Extraction step is not needed for our kit.
Pls let me know if you have any further questions. Thank you.
 Customer Reviews
  • Tested application: ELISA
    Test sample: Serum
    Species: Mouse
    Review: Good results and reproducibility in serum sample from mice in samples diluted 1:40.

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