Mouse 1,25-dihydroxyvitamin D3 (DVD/DHVD3) ELISA kit

Code CSB-E13697m
See More Details
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Lead Time 3-5 working days
Abbreviation 1,25 DHVD3
Protein Biological Process 1 Metabolism
Target Name 1,25-dihydroxyvitamin D3
Species Mus musculus (Mouse)
Sample Types serum
Detection Range 1000 fmol/L-5000 fmol/L
Sensitivity 250 fmol/L
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Competitive
Precision

Intra-assay Precision (Precision within an assay): CV%<10%

 

Three samples of known concentration were tested twenty times on one plate to assess.

 

Inter-assay Precision (Precision between assays): CV%<15%

 

Three samples of known concentration were tested in twenty assays to assess.

 

 

 

Linearity

 To assess the linearity of the assay, samples were spiked with high concentrations of Mouse1,25-dihydroxyvitamin D3 (1.25 DVD/DHVD3) in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Typical Data

 These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

References
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Q&A and Customer Reviews

Q&A
Q:

Please let us know the sulfuric acid content of [stop solution] .

A:
Thanks for your inquiry.The sulfuric acid content of [stop solution] is 4N.Pls let me know if you have any further questions. Thank you.
Q:

Can you please provide the following information about this kit:
a. Capture antibody or capture antigen – immunogen type, sequence (including species source and accession no. if available),expression host, clonality and host species.
b. Detection antibody (if applicable) - immunogen type, sequence (including species source and accession no. if available),expression host, clonality and host species.
c. Standard – if recombinant protein please specify sequence (including species source and accession no. if available) and expression host.
e. Please send the manual
f. Please send us also, if possible, your in-house data for plasma samples for customer review.

A:
Thanks for your inquiry.
CSB-E13697m
This is a competitive kit and there is only one antibody in the kit.
The plate is coated with rat monoclonal antibody.
The standard is purified 1,25 DHVD3.
I will send you the manual by e-mail.
This kit can only test serum samples. We tested serum samples before and the test value is 2000-4000fmol/L under 1:40 dilution. The final concentration is 80--160pmol/L. The manual will be sent to you by e-mail.
Pls let me know if you have any further questions. Thank you.
Q:

I am currently evaluating different 1,25-dihydroxyvitamin D3 ELISA Kits from various suppliers. Could you therefore please send me the assay protocol of your kit, in order that I can get an idea as to how your assay works? I noticed that many suppliers require an extraction step in organic solvents, but it is not clear to me whether the same is the case for your product.

A:
Thanks for your inquiry.
Extraction step is not needed for our kit.
Pls let me know if you have any further questions. Thank you.
Customer Reviews

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