Mouse Adenosine receptor A1(ADORA1) ELISA kit

Instructions
Code CSB-EL001375MO
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name adenosine A1 receptor
Alternative Names Adora1Adenosine receptor A1 ELISA kit
Abbreviation ADORA1
Uniprot No. Q60612
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 39 pg/mL-2500 pg/mL
Sensitivity 9.75 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse ADORA1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %110
Range %107-113
1:2Average %86
Range %83-89
1:4Average %98
Range %94-102
1:8Average %95
Range %90-100
Recovery
The recovery of mouse ADORA1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8884-93
EDTA plasma (n=4)10198-106
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
25002.086 2.041 2.064 1.937
12501.578 1.599 1.589 1.462
6251.069 1.089 1.079 0.952
312.50.642 0.669 0.656 0.529
1560.402 0.431 0.417 0.290
780.346 0.352 0.349 0.222
390.224 0.229 0.227 0.100
00.128 0.126 0.127
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

Target Data

Function Receptor for adenosine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase.
Gene References into Functions
  1. Loss of A1AR expression results in an increased susceptibility to noise-induced cochlear neural injury and hearing loss. PMID: 28034618
  2. Expression of the SENP2 gene was suppressed by theobromine. In vivo knockdown studies showed that AR1 knockdown in mice attenuated the anti-adipogenic effects of theobromine in younger mice. Theobromine suppresses adipocyte differentiation and induced C/EBPbeta degradation by increasing its sumoylation. PMID: 28965824
  3. The three receptor sets considered (mAChR, AR and TrkB receptors) intervene in modulating the conditions of the competition between nerve endings. PMID: 27339059
  4. Systemic inflammatory response syndrome-associated lymphopenia is initiated by A1R desensitization and adenosine-mediated inhibition of IL-15 production is part of the mechanism that accounts for the delay in leukopenia recovery in patients with severe sepsis. PMID: 28495790
  5. Results demonstrated a significant downregulation of adenosine A1 receptors in the cortex and striatum, concomitant to striatal D2R downregulation in iron deficient mice and rats. PMID: 27600688
  6. Adenosine A1A receptor and adenosine A3A receptor agonists and adenosine 5'-monophosphate cause regulated hypothermia that was characterized by a drop in total energy expenditure, physical inactivity, and preference for cooler environmental temperatures, indicating a reduced body temperature set point. PMID: 27914963
  7. over-expression of sEH enhances A1AR-dependent contraction and reduces KATP channel-dependent relaxation in MAs. These results suggest a possible interaction between sEH, A1AR, and KATP channels in regulating vascular tone. PMID: 27629787
  8. Results indicate that the adenosine A1 receptor is an important molecular component mediating hypoxic depression in adult mice and it appears to stabilize respiration of neonatal mice PMID: 26593641
  9. The findings of this study implicated a glial-neuronal circuit, mediated by Ado, neuronal AdoRA1, and glial AdK that can modulate sleep homeostasis in a manner influenced by glial metabolic state. PMID: 27030757
  10. Chronic cerebral ischemia in a mouse model induced down-regulation of adenosine A1 receptors. PMID: 25982512
  11. Activation of A3, A2A and A1 Adenosine Receptors in CD73-Knockout Mice Affects B16F10 Melanoma Growth, Neovascularization, Angiogenesis and Macrophage Infiltration PMID: 26964090
  12. A1 receptors regulate metabolism and islet endocrine and vascular functions during ageing PMID: 25835725
  13. Adenosine A1 receptor knockout mice displayed increased depressive-like behavior. PMID: 26247862
  14. data support the interpretation that adenosine A receptors localized to the pontine reticular formation significantly alter nociception. PMID: 24976513
  15. did not identify phenotypic modifications of A1AR-related effects on blood pressure, heart rate and plasma renin by differences in genetic background PMID: 25182861
  16. Interaction between angiotensin II and adenosine in VSMC normally involves A1 receptor signalling PMID: 25251152
  17. Extracellular cAMP, through its metabolite adenosine, reduced cardiomyocyte cAMP formation and hypertrophy by activating A1 adenosine receptors while delivering an antifibrotic signal to cardiac fibroblasts by A2 adenosine receptor activation. PMID: 25401477
  18. NPS evokes central antinociceptive effects by activating both A1 and A2A receptors during phase 1, but only the adenosine A2A receptor during phase 2 of the formalin test PMID: 25447449
  19. These findings indicate that adenosine activation of leukocyte adenosine A1 receptor plays a significant role in their recruitment to the infected lung and contributes to influenza pathogenesis. PMID: 24965449
  20. The A1 adenosine receptor may play a role in the development of atherosclerosis PMID: 24525840
  21. A1ARs mediate in utero caffeine effects on cardiac function and growth and that caffeine exposure leads to changes in DNA methylation. PMID: 24475304
  22. Adenosin inhibited Lipopolysaccharide-increased HIF1alpha accumulation under both normoxic and hypoxic conditions, through activation of A1 and A3 adenosine receptors. PMID: 23969284
  23. These results indicate that inosine possesses an antidepressant-like effect probably through the activation of adenosine A1 and A2A receptors PMID: 23613131
  24. Suggest that A1 adenosine receptor counteracts the A2A AR-mediated coronary flow increase during reactive hyperemia. PMID: 24043252
  25. The splicing of Adora1 and loss of Adora1 expression on alpha-cells may explain the hyperglucagonemia observed in prediabetic NOD mice and may contribute to the pathogenesis of human type 1 diabetes. PMID: 24264405
  26. Adenosine regulates bone metabolism via A1, A2A, and A2B receptors. PMID: 23682121
  27. Endogenous A1A receptor activation protects against acute ethanol-induced liver injury by reducing oxidative stress. PMID: 23692951
  28. An important role was determined for adenosine A1 receptors in RANKL-induced osteoclastogenesis. PMID: 22311477
  29. tested whether induction of cytoprotective sphingosine kinase-1 and sphingosine-1-phosphate synthesis might be the mechanism in A1 adenosine receptor-mediated renal protection from ischemia PMID: 22695326
  30. these results show that ADORA1 is enriched on neuron cells where it functions to control norepinephrin release. Activation of this receptor during penile erection results in reduced norepinephrin release and reduced cavernosal smooth muscle contraction, therefore facilitating penile erection. PMID: 22862844
  31. The presented data provide for the first time direct evidence that the upregulation of A(1)R enhances neuronal survival. PMID: 22899189
  32. The increase in blood pressure seen in A(1)-AR knockout mice is lower than that seen in wild-type mice but was increased by ANG II nonetheless. The presence of A(1)-ARs during a low dose of ANG II-infusion limits Na(+) and phosphate excretion. PMID: 22874421
  33. It was shown that adenosine A(1) receptors in the pancreas are involved in regulating the temporal patterns of insulin release. PMID: 22550063
  34. Data suggest that expression of Adora1 is too low to play a major role in regulation of lipogenic gene expression in hepatocytes; these results are in contrast to the high expression of Adora1 in cerebral cortex. PMID: 22449383
  35. caffeine alters embryonic cardiac function and disrupts the normal cardiac response to hypoxia through blockade of A1AR action PMID: 22164264
  36. The data suggested that, in the absence of A2A adenosine receptor, omega-hydroxylase induces vasoconstriction through mitogen-activated protein kinases via upregulation of adenosine A1 receptor and protein kinase C-alpha. PMID: 22160543
  37. AMP and a non-hydrolyzable AMP analog (deoxyadenosine 5'-monophosphonate, ACP) directly activated the adenosine A(1) receptor PMID: 22215671
  38. Adenosine can negatively regulate mouse duodenal motility either by activating A1 inhibitory receptors located post-junctionally or controlling neurotransmitter release via A1 or A2A receptors. PMID: 21615720
  39. pacemaking and conduction disturbances are induced via A(1)AR through concomitant stimulation of NADPH oxidase and PLC, followed by downstream activation of ERK and PKC with LCC as possible target. PMID: 21907719
  40. It was concluded that adenosine A(1)-receptors enhance preglomerular responses during NO inhibition and ANG II treatment. PMID: 21975649
  41. A1AR signaling contributes importantly to insulin-controlled glucose homeostasis and insulin sensitivity in C57BL/6 mice and is involved in the metabolic regulation of adipose tissue. PMID: 21831968
  42. results suggest that AR signaling represents a novel, endogenous modulator of BBB permeability. PMID: 21917810
  43. Both adenosine A(2A) and A(2B) receptors are required for adenosine A(1) receptor-mediated cardioprotection, implicating a role for interactions among receptor subtypes. PMID: 21743001
  44. Data suggest that adenosine deficiency may be relevant to human epilepsy and that a ketogenic diet can reduce seizures by increasing adenosine A1 receptor-mediated inhibition. PMID: 21701065
  45. Matrix metalloproteinase-mediated EGFR activation appears essential to cardiac protection and signaling via A1 adenosine receptors and preconditioning. PMID: 21460200
  46. A(1) and A(2B) receptors are both involved in differentiation to Treg, but through different mechanisms. PMID: 21557932
  47. these results suggest that A1 receptor-mediated signaling regulates EAAT2 expression in astrocytes. PMID: 21291865
  48. The results of this study suggested that both A1 as well as A2A receptor antagonists could be useful therapeutically to lower the risk of L-DOPA-induced dyskinesia in parkinson disease patients PMID: 20828543
  49. Adenosine A1 receptor-mediated inhibition of ghrelin release from the stomach is apparent when the tonic excitatory effect of adenosine is removed from the isolated perfused gastric mucosa. PMID: 20876230
  50. Data show that adenosine A1 and A2A receptors are not upstream of caffeine's dopamine D2 receptor-dependent reward-aversion effects. PMID: 20576036
  51. Adenosine A1 receptor activation is an endogenous inhibitor of the microglial response to traumatic brain injury, likely via inhibition of proliferation. PMID: 20121416
  52. Glomerular tubular balance is dependent on adenosine acting on type 1 receptors in the proximal tubule PMID: 20810615
  53. IL-6 and OSM depend on A1R function for their neuroprotective properties. PMID: 20598020
  54. Our data reveal what we believe to be a previously unrecognized distinct role of Adenosine receptor A1 for polymorphonuclear cell trafficking and endothelial integrity in a model of acute lung injury. PMID: 20729330
  55. Data suggest that the role of adenosine A(1) receptors in regulating vessel tone differs between blood vessels, and that A(1) receptor modulation of blood pressure is therefore mainly related to extravascular factors. PMID: 20132147
  56. Hematopoietic precursor cells expressed Adora1 mrna. PMID: 19249907
  57. Report very low, unquantifiable expression of adenosine A1 receptor mRNA in both normal and lipopolysaccharide-activated macrophages. PMID: 19249906
  58. critical role for adenosine in bone homeostasis via interaction with adenosine A(1)R PMID: 20181934
  59. Adenosine A1 receptor is involved in altered vascular parameters and airway hyperresponsiveness as a result of allergen exposure and adenosine aerosol challenge in sensitized mice. PMID: 20400685
  60. Myocardial adenosine A(1)-receptor-mediated adenoprotection involves phospholipase C, PKC-varepsilon, and p38 MAPK, but not HSP27. PMID: 20363896
  61. Adenosine exerts strong neuroprotective functions through the activation of adenosine A1 receptor. PMID: 20137682
  62. bones from A(1)R-knockout mice showed significantly increased bone volume in osteoclastic bone resorption PMID: 20112380
  63. Results suggest that adenosine receptor desensitization may be a mechanism for T cells to escape the general suppression during early points of T-cell activation. PMID: 19950175
  64. pre-treatment with a specific A1AR antagonist (DPCPX) attenuated the heart-rate slowing effects of adenosine in wild-type, A2AAR(-/-), or A2BAR(-/-) mice, but did not alter hemodynamic responses of A1AR(-/-) mice PMID: 19707555
  65. mediates Mg(2+) uptake in distal convoluted tubule PMID: 11704566
  66. overexpression of A1-adenosine receptor protects intact mice against myocardial infarction PMID: 11834491
  67. Oral and systemic effectiveness of A(1) antisense towards adenosine receptors in the central nervous system. PMID: 12443980
  68. Macula densa-dependent renin secretion in A1 adenosine receptor knockout mice was preserved, indicating it is not indispensable in macula densa control of the renin system PMID: 12475747
  69. enhanced expression of adenosine A1 receptor causes sinus nodal and atrioventricular nodal dysfunction and supraventricular arrhythmias PMID: 12637351
  70. that chronic A1AR deficiency diminishes the effectiveness of ANG II to constrict renal resistance vessels and to reduce GFR. PMID: 12793993
  71. Deletion of A1 receptors from hippocampal CA3 neurons abolished response to adenosine, but deletion of A1 receptors from CA1 neurons had no effect, demonstrating a presynaptic site of action PMID: 12843280
  72. Emotional instability but intact spatial cognition in adenosine receptor 1 knock out mice. PMID: 14529816
  73. endogenous or exogenous preischemic activation of A1 ARs protects against renal ischemia reperfusion injury in vivo via mechanisms leading to decreased necrosis and inflammation. PMID: 14600029
  74. mice deficient for adenosine A1 receptors lack tubuloglomerular feedback PMID: 14767772
  75. adenosine A1 receptor is induced by osmotic diuretics through activation of a xanthine oxidase pathway leading to ROS generation and promoting activation of NF-kappaB PMID: 15272017
  76. activation of A(1)ARs causes contraction of vascular smooth muscle through phospholipase C pathways and negatively modulates the vascular relaxation mediated by other adenosine receptor subtypes. PMID: 15539423
  77. A(1)AR signaling may serve to regulate the severity of pulmonary inflammation PMID: 15630442
  78. The stimulation of chloride secretion by adenosine in jejuna derived from mice lacking the adenosine receptors of A1 (A1R) and A2A (A(2A)R) or control littermates is reported. PMID: 15637180
  79. the adenosine A1 receptor plays a physiological role in inhibiting nociceptive input at the spinal level in mice. PMID: 15661449
  80. no difference in renal tubular apoptosis was detected between A(1) knockout and A(1) wild-type mice PMID: 15784841
  81. normal spatial learning and memory and hippocampal CA1 synaptic plasticity in adult adenosine A1R knockout mice, but modifications in arousal-related processes, including habituation PMID: 15858837
  82. data suggest a critical endogenous anticonvulsant action of adenosine at A1 receptors early after experimental traumatic brain injury PMID: 16121125
  83. These observations show that the developing nervous system is indeed sensitive to acute hypoglycemic injury and that A(1)AR activation contributes to damage induced by hypoglycemia. PMID: 16150954
  84. A1 adenosine receptors are indispensable for the inhibition of renin secretion by an increase in blood pressure, suggesting that formation and action of adenosine is responsible for baroreceptor-mediated inhibition of renin release. PMID: 16172432
  85. autoregulation of renal vascular resistance is less complete in A1AR-deficient mice, an effect that is presumably related to absence of TGF regulation in these animals PMID: 16263804
  86. Thalamocortical excitation is regulated by presynaptic adenosine A1 receptors and provide a mechanism by which increased adenosine levels can directly reduce cortical excitability. PMID: 16343787
  87. conclude that radiocontrast causes acute renal failure via mechanisms dependent on A1 adenosine receptors (A1AR) PMID: 16418301
  88. The adenosine- and NECA-induced increase in coronary flow in A1AR(-/-) hearts was not altered by DPCPX. These data indicate that A1AR may inhibit or negatively modulate coronary flow mediated by other AR subtypes (A2A and A2B). PMID: 16517942
  89. These data affirm the cardioprotective role of A(1)ARs and suggest that induced expression of other adenosine receptor subtypes [A(2A), A(2B), and A(3)] may participate in the response to ischemia-reperfusion in isolated murine hearts. PMID: 16679400
  90. Adenosine A1 receptor is critically involved in limiting the spread of a seizure-induced lesion and preventing the progression of status epilepticus to lethal consequences. PMID: 16750195
  91. the kinetics of adenosine receptors suggest that at early stage of peritonitis, the A(1)R dominates, and later its dominance is replaced by the G stimulatory (Gs) protein-coupled A(2A)R that suppresses inflammation PMID: 16788688
  92. The action of adenosine on constrictor A(1)-Ado receptors and dilatory A(2)-Ado receptors modulates the interaction with Ang II PMID: 16807541
  93. Adenosine attenuates glioblastoma growth acting via A(1)AR in microglia. PMID: 16951167
  94. Nitric oxide, viathe activation of NF-kappaB, serves as an endogenous regulator of A1AR, and speculate that the induction of the A1AR could counteract the cytotoxicity of nitric oxide. PMID: 16987234
  95. Adenosine A(1) receptor is involved in the regulation of heart rate, body temperature and locomotor activity, but the magnitude of the involvement is different in males and females. PMID: 17428234
  96. The A(1)R mediates the antilipolytic actions of adenosine. Deletion of the A(1)R in mice does not result in compensatory increases in G-protein-mediated antilipolytic actions of PGE(2) or nicotinic acid. PMID: 17428236
  97. Cardiac dysfunction secondary to the overexpression of TNF is associated with marked alterations in myocardial levels of adenosine and ARs PMID: 17438146
  98. A1 adenosine receptors play an essential role in protecting the embryo against hypoxia. PMID: 17522253
  99. This study shows that the A1AR knockout mice develop diabetes-induced glomerular hyperfiltration, demonstrating that the tubuloglomerular feedback mechanism is not the major cause of the development of hyperfiltration. PMID: 17581137
  100. Results show that A(1)R minimally affects muscle glucose uptake, but is important in regulating pancreatic islet function. PMID: 17869224

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Subcellular Location Cell membrane, Multi-pass membrane protein
Protein Families G-protein coupled receptor 1 family
Database Links

KEGG: mmu:11539

STRING: 10090.ENSMUSP00000043522

UniGene: Mm.298908

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