Mouse Adenosine receptor A1(ADORA1) ELISA kit

Instructions
Code CSB-EL001375MO
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application

Product Details

Target Name adenosine A1 receptor
Alternative Names RDC7, OTTHUMP00000039091
Abbreviation ADORA1
Uniprot No. Q60612
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 39 pg/mL-2500 pg/mL
Sensitivity 9.75 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse ADORA1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %110
Range %107-113
1:2Average %86
Range %83-89
1:4Average %98
Range %94-102
1:8Average %95
Range %90-100
Recovery
The recovery of mouse ADORA1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8884-93
EDTA plasma (n=4)10198-106
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
25002.086 2.041 2.064 1.937
12501.578 1.599 1.589 1.462
6251.069 1.089 1.079 0.952
312.50.642 0.669 0.656 0.529
1560.402 0.431 0.417 0.290
780.346 0.352 0.349 0.222
390.224 0.229 0.227 0.100
00.128 0.126 0.127
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days
Still Have Questions? Leave a Message or Start an on-line Chat

Target Data

Function Receptor for adenosine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase.
Subcellular Location Cell membrane, Multi-pass membrane protein
Protein Families G-protein coupled receptor 1 family
Database Links

KEGG: mmu:11539

STRING: 10090.ENSMUSP00000043522

UniGene: Mm.298908

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