Mouse Adenosine receptor A2a(ADORA2A) ELISA kit

Instructions
Code CSB-EL001376MO
Size 96T,5×96T,10×96T
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Product Details

Target Name adenosine A2a receptor
Alternative Names Adora2aAdenosine receptor A2a ELISA kit
Abbreviation ADORA2A
Uniprot No. Q60613
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.81 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse ADORA2A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %96
Range %90-100
1:2Average %93
Range %89-97
1:4Average %99
Range %94-103
1:8Average %106
Range %102-113
Recovery
The recovery of mouse ADORA2A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8984-93
EDTA plasma (n=4)9490-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1OD2AverageCorrected
20002.412 2.507 2.460 2.333
10002.042 2.156 2.099 1.972
5001.471 1.527 1.499 1.372
2500.824 0.895 0.860 0.733
1250.482 0.509 0.496 0.369
62.50.258 0.279 0.269 0.142
31.250.209 0.217 0.213 0.086
00.124 0.129 0.127
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

Target Data

Function Receptor for adenosine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.
Gene References into Functions
  1. protective role in obesity-associated nonalcoholic fatty liver disease attributable to the direct effects of A2AR on altering inflammatory and metabolic responses of macrophages and hepatocytes PMID: 29315766
  2. Study demonstrates that cognitive functions, including spatial memory and mood, are impaired due to acute hypobaric hypoxia (HH); and demonstrate that genetic inactivation of A2AR prevented spatial memory impairment in acute HH model of mice. The underlying mechanism might include microglia-mediated neuroinflammation via A2AR activation in the hippocampus. PMID: 29501623
  3. Cigarette smoke impairs A2A adenosine receptor mediated wound repair through up-regulation of Duox-1 expression. PMID: 28337995
  4. The present study evaluates the behavioral effects of pharmacological manipulation and genetic blockade of A2A R and D2 R within the frame of such a predominant striatal heteromeric population. PMID: 29053217
  5. Early synaptic deficits in the APP/PS1 mouse model of Alzheimer's disease involve neuronal adenosine A2A receptors. PMID: 27312972
  6. importance for MC5r and A2Ar expression in EAU to promote the induction of protective regulatory immunity, and the expression of MC5r and A2Ar on human immune cells, suggests that it may be possible to utilize the melanocortin-adenosinergic pathways to induce protective immunity in uveitic patients PMID: 27886238
  7. Body weight was significantly smaller in A2AR-KO mice at the age of 6 months PMID: 29064819
  8. Results show overall cognitive impairments in A2A adenosine receptor knockout mice associated with a decrease in new-born hippocampal neuron proliferation and concomitant changes in synaptic protein expression, in both the prefrontal cortex and the hippocampus. PMID: 28844595
  9. aged mice have a lower ability to cope with inflammation due to C. albicans over-colonization, associated with an inability to adaptively adjust adenosine A2A receptors density PMID: 27590517
  10. A2AR activation enhances its interaction with endothelial glutamate transporters. Inhibition of A2AR rescued dysfunction of brain endothelial glutamate transporters. PMID: 29526759
  11. The astrocytic Lrp4 plays an important role in ischemic brain injury response. Lrp4 deficiency in astrocytes seems to be protective in response to ischemic brain injury, likely because of the increased ATP release and adenosine-A2AR signaling. PMID: 29212737
  12. the potential effects of the A2A adenosine receptor (A2AR) antagonist SCH 5826 (SCH) and agonist CGS 21680 (CGS) on behavior (self-grooming), hot plate test results, and expression levels of IL-17A(+), RORgammat(+), Foxp3(+), and IL-10(+) in CD4(+) T spleen cells in BTBR and C57BL/6 (B6) mice, were investigated. PMID: 28438638
  13. Compared to adults, young WT mice failed to control S. pneumoniae infection after vaccination and this was associated with lower levels of CD73 on innate B cells. We hypothesized that pharmacological activation of A2a receptor may improve Pneumovax 23 immunization in young WT mice PMID: 29377929
  14. Absence of A2AAR increases cochlear resistance to acoustic trauma. PMID: 28034618
  15. Behavioral, anatomical and neurochemical studies to assess psychotic-like symptoms in adult male and female A2AR KO and wild-type littermates. Results bolster the adenosine hypothesis of schizophrenia by demonstrating behavioral and anatomical alterations associated with the specific deletion of adenosine A2AR. These findings resemble most of the relevant phenotype features of the human disorder. PMID: 27133030
  16. Adenosine receptor A2A drives pathological angiogenesis in the oxygen-induced retinopathy mouse model by promoting glycolysis in endothelial cells via the ERK/Akt/HIF-1alpha pathway. PMID: 28928465
  17. it is concluded that the anxiolytic-like activities of rubimetide and typical agonist peptides of the FPR2 were mediated successively by the PGD2-DP1 receptor, adenosine-A2A receptor, and GABA-GABAA receptor systems downstream of the FPR2. PMID: 27475912
  18. Study focused on the impact of A2A receptor deletion (A2A receptor knockout, A2AKO) on motivation for social exploration. Although A2AKO mice showed an anxious profile, they displayed higher levels of sociability and were less sensitive to social novelty. c-Fos immunoreactivity in A2AKO mice was higher in anterior cingulate and amygdala compared to wild type mice. PMID: 28007538
  19. data showed that A2AARs control pancreatic dysfunction in HFD-induced obesity PMID: 28765173
  20. Data indicate A2ARs exert minor effects in un-stressed myocardium and selectively suppress NFkappaB and JAK-STAT signalling and cardiac injury without influencing cardiac depression in endotoxemia. PMID: 27696085
  21. The three receptor sets considered (mAChR, AR and TrkB receptors) intervene in modulating the conditions of the competition between nerve endings. PMID: 27339059
  22. High A2aR expression is associated with Melanoma Metastasis. PMID: 28652244
  23. These novel findings indicate that adenosine suppresses REM sleep via A2AR in the OB of rodents. PMID: 27485749
  24. The findings suggest that disruption of the protective A2AR amplifies inflammation to accelerate glomerular damage and endogenous macrophage A2ARs are essential to protect from progressive kidney fibrosis. PMID: 27520357
  25. A2A AR activation on BBB endothelial cells offers a therapeutic window that can be fine-tuned for drug delivery to the brain and has potential as a CNS drug-delivery technology. PMID: 27043281
  26. this study has identified a unique role for A2aRs in regulating CD4 T cell differentiation during vaccination. PMID: 27986907
  27. these observations point to a previously unappreciated role of GS protein-coupled A2aR in regulating humoral immunity, which may be pharmacologically targeted during vaccination or pathological states in which GC-derived autoantibodies contribute to the pathology. PMID: 27974461
  28. In this study, we investigated whether blockade of the A2A adenosine receptor with a selective antagonist and a CD73 inhibitor may increase the efficacy of a dendritic cell-based cancer vaccine PMID: 28349824
  29. Results indicate that neuroprotection by caffeine in the MPTP model of Parkinson's disease relies on the A2AR, although the specific cellular localization of these receptors remains to be determined PMID: 26905951
  30. Striatopallidal adenosine A(2A) receptor signaling in the dorsomedial striatum exerts inhibitory and predominant control of goal-directed behavior by acting precisely at the time of reward. PMID: 26216520
  31. Diabetes resulted in an increased A2A/A2B receptor expression in coronary arteries which resulted in enhanced A2A/A2B-mediated increase in coronary flow observed in diabetic hearts. PMID: 26654777
  32. Thsi study demonstrated thata complex involvement of A2aR in the pathogenesis of chronic neuroinflammation in mice. PMID: 26920550
  33. In the adenosine A2A receptor knock-out mouse, pulmonary hypertension develops through activation of RhoA/ROCK signaling pathway. PMID: 26647014
  34. A2AR deletion is sufficient to prevent memory defects, hippocampal plasticity impairments as well as Tau hyperphosphorylation, in a mouse model of Alzheimer's disease PMID: 25450226
  35. A2AR signaling regulates both basal and LPS-induced DUSP1 levels in macrophages via activating the adenylate cyclase pathway. PMID: 27066978
  36. Plasminogen activation contributes to wound repair and suggest that adenosine A2A receptor activation promotes wound closure by a mechanism that depends on tPA. PMID: 25991438
  37. These results show that the dysfunction of astrocytic A2AR, by controlling GLT-I activity, triggers an astrocyte-to-neuron wave of communication resulting in disrupted glutamate homeostasis. PMID: 25869810
  38. This study showed that the recruitment of intracellular A2AR signaling in the hippocampus is sufficient to trigger memory dysfunction. PMID: 25687775
  39. Activation of A3, A2A and A1 Adenosine Receptors in CD73-Knockout Mice Affects B16F10 Melanoma Growth, Neovascularization, Angiogenesis and Macrophage Infiltration PMID: 26964090
  40. results suggest that during atherosclerosis, female mice are more susceptible to NO-dependent endothelial dysfunction and the upregulation of A2A AR may serve as a compensatory mechanism to counteract the compromised endothelial function PMID: 26201383
  41. results show that A2A receptors are necessary and sufficient to trigger memory impairment. PMID: 25939452
  42. A novel mutual suppression feedback loop between A2AR signaling and miR-214 in inflammation. PMID: 26171727
  43. ST1535 metabolites ST3932 and ST4206 are effective adenosine A2A receptor antagonists which maybe useful in treating Parkinson disease. PMID: 25936513
  44. Study shows the ability of A2AR inactivation or pharmacological blockade to control working memory deficits in the prodrome and early phases of R6/2 mice modeling Huntington's disease PMID: 25892655
  45. adenosine-induced increase in coronary flow is partially mediated by Nox2-derived H2O2, which critically depends upon the presence of A2A adenosine receptors PMID: 25911169
  46. Expression of NPP1 and 5'-nucleotidase by valve interstitial cells promotes the mineralization of the aortic valve through A2aR and a cAMP/PKA/CREB pathway. PMID: 25644539
  47. Adenosine Receptor 2A Blockade Increases the Efficacy of Anti-PD-1 through Enhanced Antitumor T-cell Responses PMID: 25672397
  48. These data suggest that the use of pharmacologic A2AR antagonists may hold therapeutic potential in diminishing the rate of cancer development. PMID: 26296573
  49. Overall, findings from this study that implicates the contribution of EETs, PPARgamma and KATP channels downstream of A2AAR to mediate enhanced vascular relaxation in response to HS diet PMID: 25739357
  50. Inhibition of small hyaluronic acid fragment activity and stimulation of A2A adenosine receptor pathway limit apoptosis and reduce cartilage damage in experimental arthritis. PMID: 25511416
  51. findings provide new insights into the anti-inflammatory mechanisms of A2AAR signaling and the potential utility of A2AAR agonists in inflammatory diseases. PMID: 26355151
  52. The results herein reported clearly document that at the neuromuscular junction of SOD1(G93A) mice there is an exacerbation of A2A receptor-mediated excitatory effects at the pre-symptomatic phase. PMID: 25093813
  53. Presynaptic adenosine A2A receptors dampen cannabinoid CB1 receptor-mediated inhibition of corticostriatal glutamatergic transmission PMID: 25296982
  54. data show that BDNF exerts neuroprotective effects toward NMDA-dependent toxicity in experimental models of Huntington's disease and that such effects require endogenous A2AR activation PMID: 23363456
  55. Extracellular cAMP, through its metabolite adenosine, reduced cardiomyocyte cAMP formation and hypertrophy by activating A1 adenosine receptors while delivering an antifibrotic signal to cardiac fibroblasts by A2 adenosine receptor activation. PMID: 25401477
  56. Adenosine doubled caspase-1 activity in brain by a pathway reliant on ATP-sensitive potassium channels, protein kinase A and the A2A adenosine receptor. A2A knockout mice were resistant to adenosine-induced anxiety. PMID: 24907587
  57. Adenosine A2A receptors in bone marrow-derived cells are important modulators of white matter lesions. PMID: 25756592
  58. A2AR mediated improvements in Per1 and Per2 expression and circadian behavior in ENT1 KO mice PMID: 24755889
  59. These data suggest the critical role of A2AR signaling pathway in inhibiting the maturation and proinflammatory function of reoxygenated dendritic cells. PMID: 25458315
  60. NPS evokes central antinociceptive effects by activating both A1 and A2A receptors during phase 1, but only the adenosine A2A receptor during phase 2 of the formalin test PMID: 25447449
  61. The results indicate that tumor-associated myeloid cells, including macrophages, DCs, and MDSCs all express immunosuppressive A2ARs that are potential targets of adenosine receptor blockers to enhance immune killing of tumors. PMID: 25377469
  62. our findings imply that the efficacious application of A(2A)R inhibitors for cancer immunotherapy may require careful dose optimization to prevent activation-induced T-cell death in tumors. PMID: 25341542
  63. Adenosine AA receptors in striatal glutamatergic terminals and GABAergic neurons oppositely modulate psychostimulant action and DARPP-32 phosphorylation. PMID: 24312250
  64. Data suggest that adenosine A2A receptor activation inhibits cell differentiation to form osteoclasts and thus could regulate bone turnover; inhibition is via cyclic AMP-dependent protein kinase-dependent inhibition of NFkappaB nuclear translocation. PMID: 23647065
  65. Inflammasome activity is sustained, after initial activation, by A(2A) receptor-mediated signalling. PMID: 24352507
  66. Data suggest the possibility to manipulate NKT-cell activity in inflammatory disorders through intervention to the adenosine-adenosine A2A Receptor A2AR pathway. PMID: 24448964
  67. CX3CL1 transiently potentiates NMDAR function though mechanisms involving A2AR activity and the release of D-serine. PMID: 23981568
  68. These results indicate that inosine possesses an antidepressant-like effect probably through the activation of adenosine A1 and A2A receptors PMID: 23613131
  69. data show that inosine acting on A2A or A3 adenosine receptors can regulate OVA-induced allergic lung inflammation PMID: 23355189
  70. Data (including data from knockout mice) suggest that Adora2a function is involved in regulating motor functions in ways that are potentially related to parkinsonism; antagonist or knockout of Adora2a improve tremors in Parkinson's disease model. PMID: 22947264
  71. Adenosine 2A receptor antagonist prevented and reversed liver fibrosis in a mouse model of ethanol-exacerbated liver fibrosis. PMID: 23874883
  72. Adenosine A2A receptor activity protects myocardial viability without modifying contractile dysfunction in cardiac injury. PMID: 22192288
  73. Suggest that A1 adenosine receptor counteracts the A2A AR-mediated coronary flow increase during reactive hyperemia. PMID: 24043252
  74. PPARgamma and A2AR could upregulate the mRNA and protein expressions of each other in lung tissues in a mouse model of acute lung injury and murine macrophages. PMID: 23712033
  75. Data indicate that combined prostaglandin E2 and adenosine treatment significantly suppressed the production of IFN-gamma and IL-17 via EP4 and adenosine A2A receptors. PMID: 23237743
  76. This coupling of astrocytic A2ARs to the regulation of glutamate transport through modulation of NKA-alpha2 activity provides a novel mechanism linking neuronal activity to ion homeostasis controlling glutamatergic activity. PMID: 24259572
  77. Data from knockout mice suggest Adora2a regulates binding of nicotinic agonists in brain; genetic ablation of Adora2a prevents nicotine-induced up-regulation of alpha7 nicotinic acetylcholine receptor without affecting alpha4beta2 nicotinic receptor. PMID: 23583933
  78. In huntingtin transgenic mice, A2AR levels were reduced from 12 weeks of age onwards, and this downregulation was concomitant with reduced 5hmC levels in the 5'UTR region of ADORA2A. PMID: 23385980
  79. This study demonistrated that a2a adennsine receptor mediated signaling is a critical pathway debilitating retinal cell death associated with traumatic optic neuropathy. PMID: 24090652
  80. Data indicate that CD73 promoted metastasis through the activation of both A2A and A2B receptors. PMID: 23964122
  81. MC5r-dependent regulatory immunity increased CD11b(+)F4/80(+)Ly-6C(low)Ly-6G(+)CD39(+)CD73(+) APCs in the spleen of post-experimental autoimmune uveoretinitis mice. These MC5r-dependent APCs require adenosine 2A receptor expression on T cells. PMID: 24043903
  82. Data show that lipopolysaccharides (LPS) upregulates adenosine A(2A) receptor A(2A)R expression is NF-kappaB-dependent. PMID: 23328845
  83. Adenosine regulates bone metabolism via A1, A2A, and A2B receptors. PMID: 23682121
  84. A2AR KO significantly exacerbated the progression of UUO-induced RIF in mice, prominently at the initial stage. PMID: 23026406
  85. Adenosine A2A receptor activation plays a role controlling the release of BDNF from activated microglia. PMID: 23363775
  86. Activation of adenosine A2A receptor alleviates renal interstitial fibrosis in obstructive nephropathy. PMID: 23585831
  87. Activation of striatal A2AR function is mediated by CD73 and contributes to control of striatal function and neurodegeneration. PMID: 23843511
  88. The results of the present study suggest that striatal A2AR is not necessary for the normal expression of learned inattention or sensorimotor gating. PMID: 23276608
  89. A2AR activation attenuates abdominal aortic aneurysm formation partly by inhibiting immune cell recruitment and reducing elastin fragmentation. PMID: 23413358
  90. ADORA2A is protective against lung injury after lipopolysaccharides and oxygen. PMID: 23349051
  91. The cystatin F expression in the activated microglia is closely associated with the effect of the A2A receptors, which may be related to the neuroinflammatory responses occurring during the pathological process. PMID: 23285090
  92. Plasma glutamate-modulated interaction of A2AR and mGluR5 on BMDCs aggravates traumatic brain injury-induced acute lung injury PMID: 23478188
  93. Adenosine A2 receptors play a regulatory role in the hyaluronan oligosaccharide exposure induced inflammatory response in chondrocytes. PMID: 22796382
  94. The work strongly suggests that pharmacological A2AR blockade can be used to diminish scarring while improving the collagen composition and tensile strength of the healed wound. PMID: 22767233
  95. These data provide evidence for a functional interaction between adenosine A2A and mGlu5 receptors in mediating the conditioned effects of cocaine but not direct cocaine-induced hyperactivity. PMID: 21816123
  96. This study suggested that extracellular adenosine acting at A(2A)Rs triggers an important neuroprotective mechanism. PMID: 22639925
  97. A2AAR expression on nonimmune cells is required for efficient development of experimental encephalitis, while A2AAR lymphocyte expression is essential for limiting the severity of the inflammatory response. PMID: 22529293
  98. Our findings support an important role for adenosine A(2A) R in modulating the acute effects of cocaine, as demonstrated by the decrease in cocaine-evoked dopaminergic transmission in the nuleus accumbens PMID: 22213208
  99. The data suggested that, in the absence of A2A adenosine receptor, omega-hydroxylase induces vasoconstriction through mitogen-activated protein kinases via upregulation of adenosine A1 receptor and protein kinase C-alpha. PMID: 22160543
  100. Adenosine can negatively regulate mouse duodenal motility either by activating A1 inhibitory receptors located post-junctionally or controlling neurotransmitter release via A1 or A2A receptors. PMID: 21615720

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Subcellular Location Cell membrane, Multi-pass membrane protein
Protein Families G-protein coupled receptor 1 family
Database Links

KEGG: mmu:11540

STRING: 10090.ENSMUSP00000101060

UniGene: Mm.333734

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