Mouse Adenosine receptor A2b(ADORA2B) ELISA kit

Instructions
Code CSB-EL001377MO
Size 96T,5×96T,10×96T
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Product Details

Target Name adenosine A2b receptor
Alternative Names Adora2bAdenosine receptor A2b ELISA kit
Abbreviation ADORA2B
Uniprot No. Q60614
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates, cell culture supernates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.81 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse ADORA2B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 100
Range % 87-104
1:2 Average % 106
Range % 98-110
1:4 Average % 96
Range % 85-100
1:8 Average % 107
Range % 98-111
Recovery
The recovery of mouse ADORA2B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 105 94-110
EDTA plasma (n=4) 100 89-103
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
2000 2.544 2.447 2.496 2.328
1000 2.082 2.144 2.113 1.945
500 1.539 1.550 1.545 1.377
250 1.081 1.054 1.068 0.900
125 0.700 0.676 0.688 0.520
62.5 0.510 0.501 0.506 0.338
31.25 0.301 0.312 0.307 0.139
0 0.166 0.170 0.168  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

Target Data

Function Receptor for adenosine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.
Gene References into Functions
  1. Obesity is related with a condition of colonic inflammation, leading to an increase of A2BR expression. A2BR, modulating the activity of excitatory tachykininergic nerves, participate to the enteric dysmotility associated with obesity. PMID: 28808842
  2. these results suggest that A2BAR stimulation inhibits the activation of ERK1/2, p38 and NF-kappaB by RANKL, which suppresses the induction of osteoclast marker genes, thus contributing to the decrease in osteoclast cell-cell fusion and bone resorption activity. PMID: 29047264
  3. the pivotal role of CXCR4- and CXCR7-inhibition in acute pulmonary inflammation, which depended on A2B-receptor signaling, is reported. PMID: 28542132
  4. Adora2B stimulation promotes FGF2 and CXCL12 expression in FAP-positive melanoma-associated fibroblasts, contributing to the creation of a tumor-promoting microenvironment. PMID: 27590504
  5. activation of adenosine A2B receptors on myeloid cells caused nociceptor hyperexcitability and promoted chronic pain via soluble IL-6 receptor trans-signaling. PMID: 27320922
  6. findings suggest that hypoxia, through HIF1A, contributes to the development and progression of pulmonary fibrosis through its regulation of ADORA2B expression on alternatively activated macrophages, cell differentiation, and production of profibrotic mediators PMID: 28701304
  7. It has been reported that signaling through hepatocellular Adora2b adenosine receptors dampens IR injury of the liver. PMID: 27404219
  8. Diabetes resulted in an increased A2A/A2B receptor expression in coronary arteries which resulted in enhanced A2A/A2B-mediated increase in coronary flow observed in diabetic hearts. PMID: 26654777
  9. A2B adenosine receptor-induced VEGF production and angiogenesis are involved in myeloid-derived suppressor cells in a mouse melanoma model PMID: 26317647
  10. Angiotensin II stimulation alters vasomotor response to adenosine in mouse mesenteric artery: role for A1 and A2B adenosine receptors PMID: 26227882
  11. our results suggest that intestinal epithelial Adora2b signaling provides protection during intestinal inflammation via enhancing mucosal barrier responses. PMID: 25850656
  12. exposure of DCs to A2BR agonist facilitated gammadelta T cell activation, leading to augmented Th17 responses and progressive EAU development. PMID: 26147733
  13. IFN-gamma priming of macrophages selectively prevents the induction of the A2bR in macrophages to mitigate sensitivity to adenosine and to prevent this regulatory transition. PMID: 26355158
  14. These findings implicate that tissue-specific targeting of Adora2b seems to be desirable when using Adora2b agonists to prevent or treat myocardial ischemia. PMID: 26136425
  15. alveolar epithelial A2B adenosine receptor signaling contributes to lung protection, and they implicate inhaled A2B adenosine receptor agonists in ALI treatment. PMID: 26188061
  16. the adenosine A2b receptor was shown to be the only one of the adenosine receptors whose cardiac expression is induced by ischemia in both mice and humans and whose function is implicated in ischemic pre- or post-conditioning PMID: 24502579
  17. activation of the ADORA2B on macrophages plays an active role in the pathogenesis of lung fibrosis and pulmonary hypertension PMID: 25318478
  18. the A2B adenosine receptor (ADORA2B) is essential for adenosine-induced SphK1 activity in human and mouse normal and sickle erythrocytes in vitro PMID: 25587035
  19. Findings support a potentially destructive role for A2BAR under intestinal ischemia/reperfusion and acute hypoxic conditions. PMID: 24966910
  20. study implicates the A2bAR as a regulator of adipocyte differentiation and the A2bAR-KLF4 axis as a potentially significant modulator of adipose biology. PMID: 24928509
  21. Thus, adenosine acts as a danger-associated molecular pattern (DAMP) that initiates helminth-induced type 2 immune responses through A2B adenosine receptor. PMID: 24629340
  22. findings reveal that excess adenosine-mediated ADORA2B signaling underlies reduced penile PDE activity by decreasing PDE5 gene expression in a HIF-1alpha-dependent manner PMID: 24614760
  23. CD73-dependent production of extracellular adenosine and endothelial Adora2b signaling protects kidney during diabetic nephropathy. PMID: 24262796
  24. found that stimulation of A2B adenosine receptors suppressed free fatty acid-induced deleterious inflammatory and metabolic activation of macrophages PMID: 24194503
  25. Studies of ventilator-induced lung injury revealed induction of the ADORA2B during ALI in vivo that was abolished following HIF inhibition or genetic deletion of Hif1a. PMID: 24391213
  26. the A2B receptor signaling linked to up-regulation of pro-angiogenic factors in cardiac Sca-1(+)CD31(-) stromal cells is essential for overall improvement of cardiac recovery seen after their transplantation to the injured heart. PMID: 23827818
  27. an important role of the A2B receptor-dependent upregulation of JunB in VEGF production and possibly other AP-1-regulated events. PMID: 24136993
  28. blockade of ADORA2B attenuates the development of a pulmonary hypertension phenotype that correlates with reduced levels of hyaluronan (HA) deposition in vessels and down-regulation of genes involved in synthesis of HA PMID: 23855769
  29. Data suggest that crosstalk pathway between ENT2 and alveolar epithelial Adora2b in lung protection during acute lung injury (ALI) opens possibilities for combined therapies targeted to this protein set. PMID: 23603835
  30. Data indicate that CD73 promoted metastasis through the activation of both A2A and A2B receptors. PMID: 23964122
  31. Adenosine regulates bone metabolism via A1, A2A, and A2B receptors. PMID: 23682121
  32. CD73 promotes the production of renal adenosine that is a prominent driver of renal hypertension by enhanced ADORA2B signaling-mediated endothelin-1 induction in a hypoxia-inducible factor-alpha-dependent manner. PMID: 23584256
  33. Activation of Adenosine A2B receptors in cortical neurons induces leukemia inhibitory factor release. PMID: 22894638
  34. Binding of A(2B)AR to specific sites on p105 prevents polyubiquitylation and degradation of p105 protein. PMID: 22767505
  35. study identified the A2bAR as a significant regulator of HFD-induced hallmarks of T2D, thereby pointing to its therapeutic potential. PMID: 22848385
  36. A2B adenosine receptor is upregulated in the peripheral lymphoid tissues of experimental autoimmune encephalomyelitis (EAE) mice. PMID: 23225885
  37. T helper (Th)2-type cells demonstrate predominance for A2B receptor expression by myeloid cells as a mechanism of development of asthma-like pulmonary inflammation. PMID: 22956582
  38. Distinct roles for hematopoietic cell A(2b) receptor in cell trafficking and for endothelial A(2b) receptor for microvascular permeability. PMID: 22707616
  39. Study identified the A2BAR as a new regulator of osteoblast differentiation, bone formation, and fracture repair. PMID: 22403399
  40. studies identify adenosine-elicited stabilization of Per2 in the control of HIF-dependent cardiac metabolism and ischemia tolerance and implicate Per2 stabilization PMID: 22504483
  41. It is proposed that A2B adenosine receptor activation may be used by L. amazonensis to inhibit dendritic cell function and evade the immune response. PMID: 22311598
  42. This studiy identified a novel regulator of sweet taste, the A2BR, which functions to potentiate sweet responses in posterior lingual taste fields. PMID: 22253866
  43. the A(2b)AR regulates liver SREBP-1, hyperlipidemia, and atherosclerosis PMID: 22144568
  44. The stimulatory effect of adenosine required primarily A(2B) receptors in activated macrophages. PMID: 21926236
  45. Adora2b receptors are significantly associated with expression of the sweet taste receptor subunit Tas1r2. PMID: 22219293
  46. blockade of A(2B)ARs enhances DC activation and CXCR3-dependent antitumor responses. PMID: 22116822
  47. adenosine augmented IL-10 production by stimulating p38 MAPK. Collectively, our results establish that A(2B)ARs augment IL-10 production by activated murine microglia. PMID: 22116830
  48. Both adenosine A(2A) and A(2B) receptors are required for adenosine A(1) receptor-mediated cardioprotection, implicating a role for interactions among receptor subtypes. PMID: 21743001
  49. lowering adenosine in wild-type mice or genetic deletion of A(2B)R in mutant mice significantly attenuated PI3K/AKT activation, eNOS phosphorylation, and subsequent impaired penile erection PMID: 21566208
  50. suggested a mechanism for putative proinflammatory effects of A(2B)AR PMID: 21593380
  51. Purinergic stimulation of cilia beat frequency requires A(2B) adenosine receptor activation, likely via protein kinase A-dependent pathway. PMID: 21622845
  52. A(1) and A(2B) receptors are both involved in differentiation to Treg, but through different mechanisms. PMID: 21557932
  53. Ablation of the A2bAR in activated macrophages increases MMP-9. A2bAR activation reduces MMP-9 expression, which depends on TNF-alpha and could contribute to the protective role of A2bAR in a vascular injury model. PMID: 21320567
  54. A(2B)R-mediated induction of IL-6 contributes to renal fibrogenesis and shows potential therapeutic targets for chronic kidney disease. PMID: 21511827
  55. Inhibition of A2BR significantly increases survival, enhances bacterial phagocytosis, and decreases IL-6 and MIP-2 levels after cecal ligation and puncture in outbred (ICR/CD-1) mice. PMID: 21242513
  56. We hypothesize that increased A(2B)R signaling in diabetes increases insulin resistance in part by elevating proinflammatory mediators. PMID: 21270276
  57. The A(2B)AR may contribute to the later stages of ischemic preconditioning dependent on the induction of stress-responsive genes. PMID: 20797398
  58. Results indicate that A(2b)AR exerts direct contractile effects but does not alter beta-adrenergic or A(1)AR antiadrenergic effects. PMID: 20935155
  59. Adenosine A2A receptor is involved in cell surface expression of A2B receptor PMID: 20926384
  60. findings confirm that both A2AR subtypes, A2aAR and A2bAR, work in concert to protect postconditioned hearts at reperfusion. PMID: 20709859
  61. Suggest that the adenosine A(2B) receptor plays an important role in mediating lung inflammation after ischemia-reperfusion by stimulating cytokine production and neutrophil chemotaxis. PMID: 20659747
  62. Hematopoietic precursor cells expressed Adora2b mrna. PMID: 19249907
  63. Activation of macrophages by lipopolysaccharide induced up-regulation of the expression of adenosine receptor A2b mrna. PMID: 19249906
  64. Platelet A2bAR is upregulated under stress in vivo, and plays a significant role in regulating ADP receptor expression, and inhibits agonist-induced platelet aggregation. PMID: 20102488
  65. TNF-alpha-induced A(2B)AR expression in colonic epithelial cells is post-transcriptionally regulated by miR27b and miR128a PMID: 20388705
  66. Studies identify an essential role of increased adenosine in the pathogenesis of penile fibrosis via A(2B)R signaling and offer a potential target for prevention and treatment of penile fibrosis, a dangerous complication seen in priapism. PMID: 19858092
  67. These findings are in agreement with previous pharmacologic studies suggesting a deleterious role for A(2B) receptor signaling in chronic lung inflammation. PMID: 19556606
  68. adenosine A2b receptor occupancy increases IL-10 production by LPS-stimulated macrophages without affecting IL-10 promoter activity and IL-10 mRNA levels, indicating a posttranscriptional mechanism PMID: 16339566
  69. These data affirm the cardioprotective role of A(1)ARs and suggest that induced expression of other adenosine receptor subtypes [A(2A), A(2B), and A(3)] may participate in the response to ischemia-reperfusion in isolated murine hearts. PMID: 16679400
  70. Results identify the A2B adenosine receptor as a critical regulator of inflammation and vascular adhesion primarily via signals from hematopoietic cells to the vasculature, focusing attention on the receptor as a therapeutic target. PMID: 16823489
  71. In chronic lung diseases associated with increased adenosine, antagonism of A2BAR-mediated responses may prove to be a beneficial therapy. PMID: 16841096
  72. A2b adenosine receptor functions as a critical regulator of signaling pathways within the mast cell, which act in concert to limit the magnitude of mast cell responsiveness when antigen is encountered. PMID: 17200408
  73. CD73-dependent adenosine generation & signaling through A2B AR is important for cardioprotection by ischemic preconditioning. PMID: 17353435
  74. proliferation-inhibiting A(2B)AR is itself an inducible receptor regulated by B-Myb. PMID: 17979185
  75. We conclude that adenosine via activation of A2B receptors evokes IL-6 release also in vivo. PMID: 18088370
  76. A2bAR has a role in regulating CXCR4 expression in vivo and in protecting against vascular lesion formation PMID: 18184815
  77. It is likely that adenosine acts through A(2B) receptors and adenylyl cyclases to stimulate CNGA2. PMID: 18292397
  78. strategies to either reduce adenosine or block A2BR activation may prove beneficial in the treatment of this disorder PMID: 18340377
  79. establish possible cross-talk between the adenosine signaling through cAMP and IFN-gamma during regulation of major histocompatibility class II transactivator expression PMID: 18359773
  80. Coronary A(2B) AR was up-regulated in mice deficient in A(2A) AR. PMID: 18423660
  81. The A(2B) receptor is a likely target of CD73-generated adenosine PMID: 18424752
  82. The effects of adenine nucleotides on bone marrow-derived dendritic cells are mediated by their degradation product, adenosine, acting on the A(2B) receptor, and will possibly lead to an impairment of Th1 response or tolerance. PMID: 18465770
  83. study confirmed the proinflammatory role of adenosine signaling via A(2B) receptors and the anti-inflammatory actions of A(2B) antagonists in mouse bone marrow-derived mast cells PMID: 18490720
  84. Blockade of adenosine A2B receptors ameliorates murine colitis. PMID: 18536750
  85. A2BAR as a novel therapeutic target for providing potent protection from renal ischemia. PMID: 18578565
  86. role of HIF-1 in ischemic preconditioning via enhancing adenosine A2b receptor purinergic signaling pathways. PMID: 18591435
  87. our data suggest that tumor cells promote their growth by exploiting A(2B) adenosine receptor-dependent regulation of VEGF in host immune cells. PMID: 18714400
  88. studies reveal a role for A2BAR signaling in attenuating ventilator-induced lung injury and implicate this receptor as a potential therapeutic target during acute lung injury PMID: 18787641
  89. LTA modulation of MMP 9 is dependent on MEK/ERK pathway and is regulated by A2A and A2B adenosine receptors. PMID: 18950865
  90. these studies demonstrate the A2BAR as a novel therapeutic target for protection during gastrointestinal ischemia/reperfusion injury. PMID: 19299692
  91. engagement of A(2B)AR modifies murine BMDC maturation PMID: 19342636
  92. Cytokine profiling of colonic tissue revealed specific deficiencies in IL-10 in Aa2br(-/-) mice relative to controls PMID: 19342675
  93. A(2B)AR(-/-) mice display increased stool retention, decreased stool frequency, delayed colonic emptying, and decreased circular muscle relaxation. RT-PCR identified A(2B)AR expression in distal colonic ganglia. PMID: 19357134
  94. a significant role for the A2bAR in the regulation of TNF-alpha, which would contribute to the anti-inflammatory actions of adenosine under vascular stress. PMID: 19375644
  95. adenosine deaminase/A(2B)R double-knockout mice exhibited enhanced pulmonary inflammation and airway destruction PMID: 19494329
  96. The major site of A(2B)R promoter activity was found to be the type II alveolar epithelial cells. PMID: 19574419

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Subcellular Location Cell membrane, Multi-pass membrane protein
Protein Families G-protein coupled receptor 1 family
Database Links

KEGG: mmu:11541

STRING: 10090.ENSMUSP00000018644

UniGene: Mm.40740

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