Mouse Agouti-signaling protein(ASIP) ELISA kit

Instructions
Code CSB-EL002212MO
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name agouti signaling protein, nonagouti homolog (mouse)
Alternative Names Asip ELISA Kit; aAgouti-signaling protein ELISA Kit; ASP ELISA Kit; Agouti coat color protein ELISA Kit; Agouti switch protein ELISA Kit
Abbreviation ASIP
Uniprot No. Q03288
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 47 pg/mL-3000 pg/mL
Sensitivity 11.7 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse ASIP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %90
Range %85-95
1:2Average %98
Range %95-102
1:4Average %102
Range %97-107
1:8Average %94
Range %91-96
Recovery
The recovery of mouse ASIP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9791-105
EDTA plasma (n=4)9388-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
30002.575 2.590 2.583 2.401
15001.787 1.668 1.728 1.546
7501.022 1.097 1.060 0.878
3750.680 0.705 0.693 0.511
187.50.449 0.455 0.452 0.270
940.286 0.267 0.277 0.095
470.220 0.234 0.227 0.045
00.175 0.189 0.182
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

Target Data

Function Involved in the regulation of melanogenesis. The binding of ASP to MC1R precludes alpha-MSH initiated signaling and thus blocks production of cAMP, leading to a down-regulation of eumelanogenesis (brown/black pigment) and thus increasing synthesis of pheomelanin (yellow/red pigment). Causes hair follicle melanocytes to synthesize phaeomelanin instead of black or brown pigment eumelanin and produces hairs with a subapical yellow band on an otherwise black or brown background when expressed during the mid-portion of hair growth.
Gene References into Functions
  1. The data confirm development of obesity and disturbance of lipid metabolism in Ay males. There were no changes in testicular function. PMID: 30188668
  2. Methylation analysis reveals that although most lines display normal methylation at IAP elements in general, the Avy IAP element is essentially unmethylated. PMID: 25191835
  3. observed variable histone patterns in the 5' long terminal repeat of the viable yellow agouti (A(vy)) metastable epiallele PMID: 20671424
  4. Data suggest that that the agouti/melanocortin signaling pathway regulates levels of peroxisome proliferator activated receptor gamma in adipocytes. PMID: 12851309
  5. AP overproduction increased the response of the pituitary adrenal system to short-restraint stress due to increased adrenal responsiveness to ACTH PMID: 15296483
  6. Local long-term mouse Agouti expression in the PVN, DMH, and LH differentially induced hyperphagia and accelerated body weight gain without apparent changes in central neuropeptide systems that are known to stimulate food intake in rats. PMID: 15537888
  7. mice with different agouti genotypes had identical basal corticosterone levels, though yellow mice, as compared with black mice, had increased corticosterone levels after restriction stress PMID: 15779332
  8. Corin plays a critical role in specifying coat color and acts downstream of agouti gene expression as a suppressor of the agouti pathway. PMID: 18057101
  9. The preimplantation embryo's growth environment can affect the postnatal expression of a defined epigenetically sensitive allele. PMID: 18562706
  10. pigment-related genes targeted by ASP and by alphaMSH PMID: 19174519
  11. study shows ASIP-MC1R signaling includes a cAMP-independent pathway through attractin and mahogunin, while the known cAMP-dependent component requires neither attractin nor mahogunin PMID: 19493315
  12. effects of the A(y) allele on mandible morphology on different strain backgrounds, DDD and B6 PMID: 19644225

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Subcellular Location Secreted
Tissue Specificity Epithelial cells of the hair follicles and the epidermis.
Database Links

KEGG: mmu:50518

STRING: 10090.ENSMUSP00000029123

UniGene: Mm.315593

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