Mouse Alpha-1-antitrypsin(SERPINA1) ELISA kit

Instructions
Code CSB-EL021053MO
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Target Name serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1
Alternative Names Serpina1a ELISA kit; Dom1 ELISA kit; Spi1-1Alpha-1-antitrypsin 1-1 ELISA kit; AAT ELISA kit; Alpha-1 protease inhibitor 1 ELISA kit; Alpha-1-antiproteinase ELISA kit; Serine protease inhibitor 1-1 ELISA kit; Serine protease inhibitor A1a ELISA kit; Serpin A1a ELISA kit
Abbreviation SERPINA1
Uniprot No. P07758
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.78 μg/mL-50 μg/mL
Sensitivity 0.195 μg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse SERPINA1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:100 Average % 90    
Range % 85-94    
1:200 Average % 89    
Range % 85-93    
1:400 Average % 84    
Range % 80-88    
1:800 Average % 102    
Range % 98-109    
Recovery
The recovery of mouse SERPINA1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 96 91-100    
EDTA plasma (n=4) 85 80-90    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
µg/ml OD1 OD2 Average Corrected    
50 2.188 2.016 2.102 1.946    
25 1.684 1.591 1.638 1.482    
12.5 1.026 1.104 1.065 0.909    
6.25 0.742 0.703 0.723 0.567    
3.12 0.446 0.458 0.452 0.296    
1.56 0.376 0.382 0.379 0.223    
0.78 0.254 0.247 0.251 0.095    
0 0.153 0.159 0.156      
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

Target Data

Function Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin.
Gene References into Functions
  1. Novel RNA-binding activity of NQO1 promotes SERPINA1 mRNA translation. PMID: 27515817
  2. this study proved that SYVN1 enhances SERPINA1(E342K)/ATZ degradation through SQSTM1-dependent autophagy and attenuates SERPINA1(E342K)/ATZ cytotoxicity. PMID: 28121484
  3. hAAT inhibits DC activation and function, and it also attenuates autoimmunity and renal damage in the MRL/lpr lupus model PMID: 27232337
  4. FBG1 degrades A1AT-Z through a Beclin1-dependent arm of autophagy. PMID: 26295339
  5. Data indicate that the scFv4B12 intrabody increased the secretion of Z alpha1-antitrypsin that retained inhibitory activity against neutrophil elastase. PMID: 25757566
  6. unchecked neutrophil serine protease activity may arise from F. tularensis targeted reduction of plasma alpha(1)-antitrysin promoting lung tissue damage facilitating increased dissemination of this bacterium in infection PMID: 22919586
  7. alpha-1 antitrypsin gene induction has a role in experimental and clinical acute kidney injury PMID: 24848503
  8. A1AT bidirectional uptake and secretion from lung endothelial cells towards the alveolar epithelium and airspaces PMID: 24743137
  9. oxidative stress is a contributing factor in the development of liver disease in this model of alpha-1-antitrypsin deficiency. PMID: 23104507
  10. Alpha 1-antitrypsin reduces inflammation and enhances mouse pancreatic islet transplant survival PMID: 22949661
  11. Data sshow that that plasmid-derived circulating hAAT protects islet allografts from acute rejection. PMID: 21670848
  12. AAT represents a novel and viable strategy to mitigate clinical graft-versus-host disease by reducing serum levels of proinflammatory cytokines and altering the ratio of donor T effector to T regulatory cells in favor of reducing the pathological process. PMID: 22203983
  13. Serpina1a serves a critical, nonredundant function during early mouse development. PMID: 21574874
  14. analysis of the effect of mutant alpha1-antitrypsin on alpha1-antitrypsin deficiency PMID: 21505264
  15. IL-8-induced hematopoietic stem cell mobilization is inhibited by serpina1 PMID: 16432201
  16. Regulator of G Signaling 16 has a role in the distinct endoplasmic reticulum stress state associated with aggregated mutant alpha1-antitrypsin Z in the classical form of alpha1-antitrypsin deficiency PMID: 17635928

Show More

Hide All

Subcellular Location Secreted
Protein Families Serpin family
Database Links

KEGG: mmu:20700

STRING: 10090.ENSMUSP00000072652

UniGene: Mm.439692

Most popular with customers

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

© 2007-2020 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1