Mouse Amyloid beta A4 protein(APP) ELISA kit

Code CSB-EL001950MO
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P12023
Lead Time 5-7 working days
Abbreviation APP
Protein Biological Process 1 Apoptosis/Autophagy
Target Name amyloid beta (A4) precursor protein
Alias AAA, ABETA, ABPP, AD1, APPI, CTFgamma, CVAP, PN2, OTTHUMP00000096096|amyloid beta A4 protein|beta-amyloid peptide|cerebral vascular amyloid peptide|peptidase nexin-II|protease nexin-II
Species Mus musculus (Mouse)
Protein Biological Process 3 Apoptosis
Sample Types serum, plasma, tissue homogenates
Detection Range 15.6 ng/mL-1000 ng/mL
Sensitivity 3.9 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Neuroscience
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This gene encodes a cell surface receptor and transmembrane precursor protein that is cleaved by secretases to form a number of peptides. Some of these peptides are secreted and can bind to the acetyltransferase complex APBB1/TIP60 to promote transcriptional activation, while others form the protein basis of the amyloid plaques found in the brains of patients with Alzheimer disease. Mutations in this gene have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis (cerebral amyloid angiopathy). Multiple transcript variants encoding several different isoforms have been found for this gene.
HGNC 622
RGD 1306488
MGI 1914134
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse APP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 87
Range % 84-92
1:2 Average % 102
Range % 96-107
1:4 Average % 93
Range % 88-97
1:8 Average % 95
Range % 89-99
Recovery
The recovery of mouse APP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 97 93-101
EDTA plasma (n=4) 102 92-105
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
1000 2.213 2.342 2.278 2.160
500 1.586 1.665 1.626 1.508
250 1.060 1.097 1.079 0.961
125 0.673 0.683 0.678 0.560
62.5 0.407 0.412 0.410 0.292
31.2 0.281 0.294 0.288 0.170
15.6 0.195 0.200 0.198 0.080
0 0.117 0.118 0.118  
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Function Functions as a cell surface receptor and performs physiological functions on the surface of neurons relevant to neurite growth, neuronal adhesion and axonogenesis. Involved in cell mobility and transcription regulation through protein-protein interactions. Can promote transcription activation through binding to APBB1-KAT5 and inhibit Notch signaling through interaction with Numb. Couples to apoptosis-inducing pathways such as those mediated by G(O) and JIP. Inhibits G(o) alpha ATPase activity (By similarity). Acts as a kinesin I membrane receptor, mediating the axonal transport of beta-secretase and presenilin 1. May be involved in copper homeostasis/oxidative stress through copper ion reduction. Can regulate neurite outgrowth through binding to components of the extracellular matrix such as heparin and collagen I and IV (By similarity). The splice isoforms that contain the BPTI domain possess protease inhibitor activity. Induces a AGER-dependent pathway that involves activation of p38 MAPK, resulting in internalization of amyloid-beta peptide and leading to mitochondrial dysfunction in cultured cortical neurons (By similarity). Provides Cu(2+) ions for GPC1 which are required for release of nitric oxide (NO) and subsequent degradation of the heparan sulfate chains on GPC1.
Subcellular Location Membrane, Single-pass type I membrane protein, Membrane, clathrin-coated pit
Protein Families APP family
Tissue Specificity Isoform APP770 is expressed in kidney. Isoform APP751 is widely expressed. Isoform APP695 is expressed in brain, kidney and liver. Isoform APP695, isoform APP714 and isoform APP751 are expressed in several different brain regions including hippocampus, su
Database Links

KEGG: mmu:11820

STRING: 10090.ENSMUSP00000005406

UniGene: Mm.277585

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