Mouse Annexin A7(ANXA7) ELISA kit

Instructions
Code CSB-EL001848MO
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name annexin A7
Alternative Names Anxa7 ELISA kit; Anx7Annexin A7 ELISA kit; Annexin VII ELISA kit; Annexin-7 ELISA kit; Synexin ELISA kit
Abbreviation ANXA7
Uniprot No. Q07076
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.81 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse ANXA7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 93
Range % 86-98
1:2 Average % 97
Range % 92-104
1:4 Average % 84
Range % 81-88
1:8 Average % 89
Range % 83-95
Recovery
The recovery of mouse ANXA7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 101 97-106
EDTA plasma (n=4) 91 88-95
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
2000 2.723 2.877 2.800 2.602
1000 2.155 2.200 2.178 1.980
500 1.467 1.475 1.471 1.273
250 0.852 0.809 0.831 0.633
125 0.474 0.455 0.465 0.267
62.5 0.385 0.386 0.386 0.188
31.25 0.283 0.288 0.286 0.088
0 0.197 0.199 0.198  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

Target Data

Function Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis.
Gene References into Functions
  1. Study demonstrates that annexin A7 protein has an important role in gastric cancer. Silencing the expression of ANXA7 in vitro and in vivo induces the apoptosis of BGC823 gastric cancer cells and, importantly, inhibits the growth of tumor xenografts in mice. PMID: 28176245
  2. Annexin A7 deficiency upregulates FGF23 plasma levels, an effect paralleled by increased corticosterone plasma levels, as well as decreased 1,25(OH)2 D3 and PTH plasma levels. PMID: 27871077
  3. alterations in serum Annexin A7 expression may be of prognostic relevance in hepatocarcinoma lymphatic metastasis PMID: 26256045
  4. Ezrin may play a role in regulating lymphatic metastasis in hepatocellular carcinoma and might be inversely associated with A7 expression. PMID: 27914826
  5. Annexin A7 formed a protein complex with BAG4 in mouse hepatocarcinoma cells; Annexin A7 modulated BAG4 and BAG4-binding proteins in mitochondrial apoptosis. PMID: 26349959
  6. Decreased expression of Annexin A7 inhibits the proliferation of hepatocarcinoma cells by way of the intrinsic mitochoindrial pathway. PMID: 25776493
  7. annexin A7 influences insulin sensitivity of cellular glucose uptake in a COX-dependent fashion. PMID: 24903239
  8. Clic1 have a role in migration and invasion in hepatocarcinoma maybe via modulating the expression of Annexin A7 and Gelsolin. PMID: 25661391
  9. ANXA7 is an important factor in tumors with the lymphatic metastasis. PMID: 25070395
  10. Annexin A7 and galectin-3 are involved in cell migration and invasion regulation in mouse hepatocellular carcinoma cell lines. PMID: 24373698
  11. Annexin 7-sensitive cyclooxygenase activity contributes to the stimulation of gastric acid secretion by glucocorticoids. PMID: 24334852
  12. Both annexin A7 and integrin beta4 were essential for small molecule, 6-amino-2, 3-dihydro-3-hydroxymethyl-1, 4-benzoxazine-induced autophagy. PMID: 24007983
  13. Annexin A7 expression was able to inhibit HCC lymph node metastasis, whereas knockdown of Annexin A7 expression significantly induced HCC metastasis to local lymph nodes. PMID: 24188284
  14. Results suggest that RACK1-Annexin A7 interaction may be one of the means by which RACK1 and Annexin A7 influence the metastasis potential of mouse hepatocarcinoma cells in vitro. PMID: 24491534
  15. Data indicate the role of annexin A7 in the regulation of cardiac NFAT activity and hypertrophic response following cardiac stress conditions. PMID: 24508799
  16. ANXA7 is a critical factor in the development of lymphatic metastasis in hepatocarcinoma progression PMID: 23540285
  17. Anx7(+/-) mutation is associated with alterations in the signaling pathways related to these receptors PMID: 22613970
  18. Annexin A7 was expressed in a development-dependent manner in the spermatogonia of the prepubertal mice and in the type-A single (As) and type-A paired (Apr) spermatogonia of adult mice. PMID: 21735650
  19. Lack of annexin A7 sensitizes erythrocytes for "proapoptotic" Ca(2+) overload, an effect shortening the life span of the affected erythrocytes, leading to anemia. PMID: 20490540
  20. A7 and A11 have sequence repeats that form novel structures, called YP pro-beta helices, that are characterized by an ability to interact with one another PMID: 20093729
  21. Anx7 has a role in enabling cells in the adrenal gland to discriminate between the fed and fasted states in vivo PMID: 12438089
  22. reduced gene dosage in the Anx7(+/-) islet, may substantially contribute to the mechanism of the loss of genomic discrimination, in vivo, between the fed and fasted states PMID: 12606813
  23. The role of annexin A7 in platelets and red blood cells was studied using annexin A7 knockout mice. PMID: 12925238
  24. haploinsufficiency of Anx7 expression appears to drive disease progression to cancer because of genomic instability through a discrete signaling pathway involving other tumor suppressor genes, DNA-repair genes, and apoptosis-related genes PMID: 14608035
  25. involvement of annexin A7 in Ca(2+)-dependent signaling or Ca(2+) homeostasis in astrocytes PMID: 14644162
  26. Annexin A7 is translocated to nuclei of cells in early brain development and localized in the nuclei of neuronal cells in adult animals. PMID: 15819996
  27. Annexin A7 deficiency causes severe electrical instability in the murine heart, including conduction disturbances and anisotropy of impulse propagation. PMID: 17662970

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Protein Families Annexin family
Database Links

KEGG: mmu:11750

STRING: 10090.ENSMUSP00000066035

UniGene: Mm.280231

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