Mouse Aquaporin 4,AQP-4 ELISA Kit

Instructions
Code CSB-E08256m
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name aquaporin 4
Alternative Names Aqp4Aquaporin-4 ELISA Kit; AQP-4 ELISA Kit; Mercurial-insensitive water channel ELISA Kit; MIWC ELISA Kit; WCH4 ELISA Kit
Abbreviation AQP4
Uniprot No. P55088
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse AQP-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %90
Range %86-94
1:2Average %84
Range %80-88
1:4Average %89
Range %84-93
1:8Average %105
Range %100-110
Recovery
The recovery of mouse AQP-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9490-98
EDTA plasma (n=4)9894-102
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
102.765 2.627 2.696 2.573
52.189 2.336 2.263 2.140
2.51.428 1.521 1.475 1.352
1.250.682 0.715 0.699 0.576
0.6250.327 0.347 0.337 0.214
0.3120.254 0.239 0.247 0.124
0.1560.218 0.227 0.223 0.100
00.126 0.120 0.123
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Target Data

Function Forms a water-specific channel. Osmoreceptor which regulates body water balance and mediates water flow within the central nervous system.
Gene References into Functions
  1. The Aqp4 pool is drastically reduced in the skeletal muscle of mdx3cv mice, which have no dystrophin. PMID: 18284610
  2. Aquaporin 4 mislocalization away from astroglia and glia limitans delays the onset of brain edema PMID: 12232046
  3. AQP4 and alpha1-syntrophin colocalized frequently at the astrocyte membrane, especially at the perivascular astrocyte endfoot processes PMID: 12233788
  4. plays a critical role in inner ear fluid homeostasis and hearing PMID: 12359252
  5. role of accumulation in astroglial end-feet in dissipation of bidirectional water flow between blood and brain PMID: 12578959
  6. AQP-4 provides the principal route for water transport in astrocytes. PMID: 14576087
  7. water flux through perivascular AQP4 is needed to sustain efficient removal of K+ after neuronal activation PMID: 14597704
  8. AQP4 is expressed in brain endothelial cells & in the perivascular membranes of astrocyte endfeet. There are 3 distinct & serially coupled pools of AQP4 at the brain-blood interface. PMID: 14734638
  9. Sarcolemma vesicles from mdx mice show AQP4 loss from the plasma membrane & a big reduction in water permeability, implying a major role for aquaporins in skeletal muscle physiology & involvement of AQP4 in molecular changes in muscles of DMD patients. PMID: 15033928
  10. higher-order organization of AQP4 increases single-channel osmotic water permeability by one order of magnitude and that differential cellular expression levels of the two isoforms could regulate this organization. PMID: 15149973
  11. AQP4-mediated transcellular water movement is crucial for fluid clearance in vasogenic brain edema. PMID: 15208268
  12. a novel role for AQP4 as a regulator of brain extracellular space PMID: 15371505
  13. AQP4 deletion in mice is neuroprotective in a transient ischemia model of retinal injury, suggesting the possible use of AQP4 inhibitors in retinal vascular occlusive and ischemic diseases. PMID: 15557457
  14. AQP4-labeled astrocytic processes surround blood vessels, neuronal perikarya and processes, and both asymmetric and symmetric synapses: the protein may be involved in the regulation of water fluxes around both inhibitory and excitatory synapses. PMID: 15789430
  15. Data show that the sustained inhibition of gastric secretion due to tumours producing PYY/enteroglucagon in transgenic mice is associated with an increase in AQP4 expression and a down-regulation of H(+)/K(+)-ATPase in parietal cells. PMID: 15898955
  16. aquaporin-4 has species-specific roles in astrocytes and a functional relationship with Connexin43 PMID: 16103109
  17. Protective effect of AQP4 on brain swelling in bacterial abscess. AQP4 induction may reduce vasogenic edema associated with cerebral infection. PMID: 16181429
  18. Lead exposure induced a significant, 40%, increase in water permeability in astrocytes expressing AQP4, but had no effect on permeability in astrocytes not expressing AQP4. May contribute to acute lead intoxication. PMID: 16203098
  19. The lack of AQP4 expression is accompanied by sex- and region-specific alterations in brain amino acid and monoamine metabolism in mice. PMID: 16237719
  20. the involvement of aquaporin-4 in astroglial cell migration, which occurs during glial scar formation in brain injury, stroke, tumor and focal abscess. PMID: 16303850
  21. These results implicate AQP4 in the expression and termination of seizure activity and support the hypothesis that AQP4 is coupled to potassium homeostasis in vivo. PMID: 16470808
  22. These results suggest that AQP4, but not AQP1 or AQP9, may play an important role in water movement associated with the pathophysiology of edema after transient cerebral ischemia in the mouse. PMID: 16511868
  23. Modulation of AQP4 expression or function may be a novel therapeutic strategy for a variety of cerebral disorders including stroke, tumor, infection, hydrocephalus, epilepsy, and traumatic brain injury. PMID: 16564496
  24. A significant increase in the expression levels of AQP1 and AQP4 was found in mice at 270 dpi, the time corresponding with the appearance of PrP(res) immunoreactivity in Western blots and typical spongiform lesions in the brain. PMID: 16871401
  25. These findings provide direct evidences for the first time that aquaporin-4 plays an important role in the function of glutamate transporters. PMID: 17074507
  26. These findings reveal for the first time that AQP4 regulates not only water and ion homeostasis but also the functions of ovarian hormone and neurotransmitter. PMID: 17196551
  27. AQP4 showed a heterogeneous distribution in the central nervous system, but in some areas, an inverse correlation between AQP4 and extracellular matrix components has been observed, suggesting a complementary role for maintenance of water homeostasis PMID: 17349777
  28. This study demonstrates that AQP4 is important in the MPTP neurotoxic process and indicates that the therapeutic strategy targeted to astrocytic modulation with AQP4 may offer a great potential for the development of new treatment for PD. PMID: 17353068
  29. In conclusion, AQP4 mediates bidirectional transport of water across astrocytes during HI and reoxygenation. AQP4 manipulation may serve as a novel therapeutic strategy during different periods of hypoxic-ischemic brain edema in neonates. PMID: 17437301
  30. Evidence against functionally significant AQP4 modulation of Muller cell Kir4.1 potassium channel functionin retinal Muller cells. PMID: 17525153
  31. aquaporin-4 participates in the regulation of neurotransmitter release induced by depolarizing stimuli. PMID: 17611025
  32. A marked increase in AQP4 was observed in mice at the terminal stage of BSE, when they had a wide range of clinical signs, whereas no increase could be observed in the early stage before the onset of the clinical signs. PMID: 17868659
  33. This study report multiple lines of evidence against functionally significant AQP4-Kir4.1 interactions. PMID: 17869537
  34. Palmitoylated dystrobrevin restored alpha-syntrophin and aquaporin-4 (AQP4) to the mdx sarcolemma but was unable to recruit beta-dystroglycan or the sarcoglycans PMID: 18057022
  35. AQP4 is essential for the maintenance of blood-brain barrier integrity. PMID: 18281883
  36. Muller glial cells respond to excessive light with an alteration in the localization of aquaporin-4 protein; this alteration is thought to be a response to the edema in the outer retina and may support the resolution of edema. PMID: 18328627
  37. AQP4 deficiency alleviates proinflammatory cytokine release from astrocytes, in association with the SPHK1/MAPK/AKT pathway. PMID: 29956748
  38. Low expression of AQP4 is associated with Human immunodeficiency virus-associated nephropathy. PMID: 29608911
  39. This study demonstrated that the significant improvement in blood-brain barrier (BBB) permeability was observed in the AQP4-deficient ALS mouse model. PMID: 29154923
  40. Brain water content decreased following treatment with 3% HS relative to the TBI group. This was accompanied by decreases in AQP4, TNF-alpha, and IL-1beta mRNA and protein levels. PMID: 29600800
  41. Treatment with goreisan significantly decreased both brain water content and AQP4 expression in the ischemic brain at 24 hours after middle cerebral artery occlusion. PMID: 29153303
  42. The findings of this study demonstrated a novel molecular mechanism involving the SUR1-TRPM4-AQP4 complex to account for bulk water influx during astrocyte swelling. PMID: 28906027
  43. The findings of this study suggest that AQP4 KO leads to increased aggregation of Cx43 into gap junctions and provide a putative mechanistic basis for the enhanced tracer coupling in hippocampi of AQP4 KO mice. PMID: 28551776
  44. Interaction of the IgG-AQP4 complex with FcgammaRs triggers coendocytosis of the excitatory amino acid transporter 2. PMID: 28461494
  45. The diffusive and AQP4-independent solute transport in rodent brain parenchyma has been demonstrated. PMID: 28826498
  46. Present study demonstrated that AQP4 depolarization is a widespread pathological condition and may contribute to motor neuron degeneration in ALS. PMID: 28627708
  47. Dataindicate that astrocytes in the substantia nigra differ from those in neocortex by showing a higher level of aquaporin-4, particularly in those endfoot membrane domains that mediate water exchange between brain and blood. PMID: 28735099
  48. AQP4-specific T cells contribute to AQP4-targeted CNS autoimmunity PMID: 27940915
  49. AQP-4 expression was significantly elevated in the ipsilateral hemisphere in the first 24h following cerebral cortical injury in mice and this could be correlated with worsening of neurological function. Over the next 48h, there was a trend towards decrease in AQP-4 expression that was associated with partial recovery of neurological function. PMID: 28645746
  50. Study shows that the size of the aquaporin-4 (Aqp4) pool differs considerably between brain regions, roughly mirroring regional differences in Aqp4 mRNA copy numbers. PMID: 27629271
  51. tg-ArcSwe mouse model of Alzheimer's disease demonstrated a robust upregulation of AQP4 expression in areas of plaques. Compared to GFAP, AQP4 appeared predominantly at later stages of plaque formation, in older mice, and within the processes of astrocytes. In combination with GFAP, AQP4 differentiated plaques into three progression stages. PMID: 28222512
  52. in vivo studies demonstrated that LPS-induced activated microglia did not express AQP4, and in vitro that AQP4 deficiency inhibited astrocyte activation and reduced the release of proinflammatory cytokines from astrocytes. PMID: 28427055
  53. The present data point to a salient glial contribution to CSD and identify AQP4 as a new target for therapy. PMID: 28365776
  54. Aquaporin-4 (AQP4) water channels in astrocytes regulate basal brain water content. PMID: 27751903
  55. Apelin-13 protects blood brain barrier from disruption after cerebral ischemia both morphologically and functionally, which is highly associated with the increased levels of AQP4, possibly through the activation of ERK and PI3K/Akt pathways. PMID: 28402976
  56. Aquaporin-4 has two isoforms, M1 and M23, whose transcripts are driven by distinct promoters. A fragment located between exons 0 and 1 of the aquaporin-4 gene, which had been thought to be the promoter for M23, lacked enhancers functioning in astrocytes. When the astrocyte-specific enhancer (ASE) of the M1 promoter is connected to the putative M23 core promoter, it also works in astrocytes. PMID: 29125630
  57. In anhedonic mice, the expression of the water channel aquaporin 4 (AQP4), a factor in glymphatic pathway dysfunction, was down-regulated in cortex and hippocampus. PMID: 27837334
  58. The results of this study that AQP4 and GLT1 do not have a strong physical interaction between them and are, instead, differentially regulated. PMID: 28078912
  59. Study suggests that astaxanthin may exert neuroprotection following traumatic brain injury by ameliorating AQP4/NKCC1-mediated cerebral edema, and NKCC1 inactivation inhibitions the upregulation of AQP4 after traumatic brain injury. PMID: 27581370
  60. this study identified and fine mapped the major T-cell epitope of AQP4 and observed that in a transfer model, AQP4-specific T cells alone induced an encephalomyelitic syndrome, however, only in the additional presence of anti-AQP4 antibodies, central nervous system lesions are reminiscent of neuromyelitis optica PMID: 28058717
  61. Study found that aquaporin-4 deficiency facilitated fear memory extinction and NMDA receptors-dependent long-term depression in the CA3-CA1 hippocampal pathway. PMID: 27373674
  62. Study demonstrated evidence of beta-dystroglycan cleavage by matrix metalloproteinase-2/-9 in permanent middle cerebral artery occlusion mouse brains; this cleavage was implicated in aquaporin-4 redistribution and brain edema in cerebral ischemia. PMID: 27038751
  63. This study demonstrated that AQP4 dorsal hippocampal protein expression was significantly downregulated at 1day post epilepsy and was followed by a gradual return to baseline levels with a significant increase in ipsilateral protein levels by 30days post epilepsy. PMID: 27155358
  64. The major findings in this study were (1) a higher AQP4 immunogold labeling density over parenchymal astrocytic membranes in human subjects than in mice, and (2) a lower degree of AQP4 immunogold polarization to perivascular astrocytic endfoot membranes in humans compared with mice. In the human individuals, endfoot AQP4 polarization did not differ between capillary and arteriolar vessel segments. PMID: 28317216
  65. TRPV4-AQP4 interactions 'turbocharge' astroglial sensitivity to small osmotic gradients PMID: 26760501
  66. Elevation of AQP4 and selective cytokines in experimental autoimmune encephalitis mice provides some potential biomarkers in optic neuritis and demyelinating diseases. PMID: 26884844
  67. This study provided the AQP4 modulates astrocyte-to-microglia communication in neuroinflammation. PMID: 26774050
  68. AQP4 attenuates Abeta pathogenesis despite its potentially inflammatory side-effects, thus serving as a promising target for treating Alzheimer's disease PMID: 26526066
  69. The induction of antibodies to an AQP4 epitope in mice immunized with the TAX1BP1-derived peptide suggests that a latent HTLV-1 infection could lead to TAX1BP1 antigen presentation and the production of anti-AQP4 antibodies in human neuromyelitis optica. PMID: 26287441
  70. mildly improved outcome in AQP4(-/-) mice following cortical impact injury results from reduced cytotoxic brain water PMID: 25790314
  71. glial AQP4 is involved in the susceptibility differences of dopamine neurons between the substantia nigra and ventral tegmental area PMID: 26748031
  72. Study characterized the brain distribution of aquaporin-4 and found that expression was high in the cerebellum and areas associated with water movement PMID: 26489685
  73. These results demonstrate that AQP4 effects on [K(+)]e and ECS volume dynamics accelerate CSD propagation. PMID: 25944186
  74. AQP4 expression increased after traumatic brain injury and was positively correlated with edema and neurological impairments. PMID: 26039099
  75. miR-29b overexpression reduced blood-brain barrier disruption aftear ischemic stroke via downregulating AQP-4 PMID: 26126866
  76. The data of this study suggested that AQP4 modulates [K(+)]o during synaptic stimulation through its effect on extracellular space volume. PMID: 24744149
  77. AQP4 has an association with the immunoregulation of the liver granuloma formation, which may confer a new option for schistosomiasis treatment. PMID: 25604731
  78. Data showed that AQP4-reactive T cells are sufficient to trigger an NMO-like disease in mice. PMID: 25990016
  79. AQP4 drives calcium influx via TRPV4 in the glial end foot, which regulates expression of Aqp4 and Kir4.1 genes and facilitates the time course and amplitude of hypotonicity-induced swelling PMID: 26424896
  80. Mesenchymal stem cells protected BBB integrity by reducing the apoptosis of astrocytes after ischemic attack, which was due to the attenuation of inflammatory response and downregulation of aquaporin-4 expression via p38 signaling pathway PMID: 25100404
  81. The results suggest a role for AQP4-mediated water flow in support of washout of metabolites PMID: 23346911
  82. AQP4 deletion increases apoptosis following intracerebral hemorrhage PMID: 25359421
  83. The results of this study suggest that AQP4 expression accelerates GFAP and TN-C expression in activated astrocytes induced by a stab wound in the mouse brain and LPS-stimulated primary culture of astrocytes. PMID: 25174305
  84. Deletion of the aquaporin-4 gene decreased AMP-dependent kinase expression and Akt kinase phosphorylation in the heart PMID: 24792367
  85. Rotavirus diarrhea is associated with the downregulation of AQP1, -4, and -8 expression. PMID: 25160756
  86. AQP4 is expressed in the embryonic mouse brain starting at E16. PMID: 24915007
  87. AQP4 mitigates spinal cord damage and ameliorates retrograde degeneration of rubrospinal neurons by promoting edema clearance and glial scar formation after spinal cord injury. PMID: 24390474
  88. Results suggest that ovariectomy and D-gal injection induced oxidative damage results in compensatory increases of AQP4 expression, and deficiency of AQP4 exacerbates brain oxidative stress and memory deficits PMID: 21281561
  89. Retinas in AQP4 knockout mice showed higher glutamate levels than that in wild-type mice upon light damage. Study revealed a novel role of AQP4 in regulating glutamate metabolism. PMID: 24368741
  90. Findings suggest AQP4 involves the pathogenesis of depression by modulating astrocytic function and adult neurogenesis, highlighting a novel profile of AQP4 as a potential target for the treatment for depression PMID: 24422972
  91. we discovered the specific expression of AQP4 in satellite glial cells in sensory ganglia in the peripheral nervous system. PMID: 25124666
  92. This study demonistrated that Aquaporin-4 deletion in mice reduces brain disorder in experimental acute liver failure. PMID: 24321433
  93. knockout mouse studies showed water influx into the CSF is regulated by AQP-4, and not by AQP-1 PMID: 24231830
  94. AQP4 may play an inhibitory role in postischemic inflammation PMID: 23132680
  95. This study showed aquaporin-4 regulates aversive motivation of spatial memory in Morris water maze in mice. PMID: 24165567
  96. up-regulation of pro-inflammatory cytokines was significantly attenuated in AQP4/KO mice. PMID: 23428384
  97. Ordered disorder of the astrocytic dystrophin-associated protein complex in the norm and pathology. PMID: 24014171
  98. Findings suggest that the neuroprotective effect of AQP4 deletion in global cerebral ischemia involves reduced astrocyte swelling and brain water accumulation, resulting in reduced BBB disruption, inflammation, and neuron death. PMID: 24186965
  99. Foxo3a induces cerebral edema by increasing the AQP4 level in the controlled cortical impact model of traumatic brain injury in mice. PMID: 24174672
  100. The Aqp4 deletion reduces the expression of perivascular glial scaffolding proteins without affecting the endothelial barrier. Our data also indicate that AQP4 and alpha-syntrophin are mutually dependent upon each other for proper perivascular expression. PMID: 22131281

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Subcellular Location Membrane, Multi-pass membrane protein
Protein Families MIP/aquaporin (TC 1.A.8) family
Database Links

KEGG: mmu:11829

STRING: 10090.ENSMUSP00000078088

UniGene: Mm.250786

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