Mouse Aspartyl/asparaginyl beta-hydroxylase(ASPH) ELISA kit

Instructions
Code CSB-EL002226MO
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name aspartate beta-hydroxylase
Alternative Names Asph ELISA Kit; BahAspartyl/asparaginyl beta-hydroxylase ELISA Kit; EC 1.14.11.16 ELISA Kit; Aspartate beta-hydroxylase ELISA Kit; ASP beta-hydroxylase ELISA Kit; Peptide-aspartate beta-dioxygenase ELISA Kit
Abbreviation ASPH
Uniprot No. Q8BSY0
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 3.12 pg/mL-200 pg/mL
Sensitivity 0.78 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse ASPH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %92
Range %84-98
1:2Average %93
Range %89-102
1:4Average %89
Range %81-100
1:8Average %92
Range %85-102
Recovery
The recovery of mouse ASPH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9788-105
EDTA plasma (n=4)9790-102
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
2002.197 2.121 2.159 1.977
1001.372 1.342 1.357 1.175
500.846 0.861 0.854 0.672
250.505 0.528 0.517 0.335
12.50.372 0.362 0.367 0.185
6.250.277 0.259 0.268 0.086
3.120.224 0.227 0.226 0.044
00.183 0.180 0.182
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

Target Data

Function Isoform 1
Gene References into Functions
  1. Results predict that junctin ablation, or mutations that alter its structural attributes, may mimic the etiology of catecholaminergic polymorphic ventricular tachycardia by causing enhanced diastolic Ca2+ leak in the presence of beta-adrenergic activation. PMID: 22025663
  2. in skeletal muscle the disruption of Tdn/CASQ link has a more profound effect on jSR architecture and myoplasmic Ca(2+) regulation than Jct/CASQ association. PMID: 22768324
  3. Ca(2+) and JNT-dependent disassembly of the CSQ2 polymer PMID: 22123818
  4. junctate may play an important role in the regulation of sarcoplasmic reticulum Ca(2+) cycling through the interaction with SERCA2a in the murine heart. PMID: 19896466
  5. evaluation of role in vivo PMID: 11773073
  6. Cardiac remodeling and atrial fibrillation in transgenic mice overexpressing junctin PMID: 12154005
  7. To gain more insight into the underlying mechanisms of impaired contractile relaxation in transgenic mice with cardiac-specific overexpression of junctin (TG), we studied cellular Ca(2+) handling in these mice. PMID: 16289269
  8. Junctin is an essential regulator of sarcoplasmic reticulum Ca release and contractility in normal hearts. Ablation of junctin is associated with aberrant Ca homeostasis, which leads to fatal arrhythmias PMID: 17224479
  9. Data suggest that junctate over-expression is associated with an increase in the sarcoplasmic reticulum Ca2+ storage capacity and releasable Ca2+ content and support a physiological role for junctate in intracellular Ca2+ homeostasis. PMID: 17516551
  10. review of recent findings concerning the expressional regulations and the proposed functions of junctin and junctate PMID: 17706594
  11. Junctate-1 in the heart is closely linked to the homeostasis of E-C coupling proteins and a sustained increase of junctate-1 expression leads to a severe cardiac remodeling and arrhythmias. PMID: 18353357
  12. involved in maintaining sarcoplasmic reticulum Ca2+ store size in skeletal myotubes PMID: 18620751
  13. Partial downregulation of junctin enhances cardiac calcium cycling without eliciting ventricular arrhythmias in mice. PMID: 19286959
  14. analysis of aspartyl (asparaginyl) beta-hydroxylase monoclonal antibodies PMID: 19663697

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Subcellular Location Isoform 1: Endoplasmic reticulum membrane, Single-pass type II membrane protein, SUBCELLULAR LOCATION: Isoform 2: Sarcoplasmic reticulum membrane, Single-pass type II membrane protein
Protein Families Aspartyl/asparaginyl beta-hydroxylase family
Tissue Specificity Isoform 1 is detected in heart, liver and ovary (at protein level). Detected in heart ventricle. Isoform 1 is widely expressed. Isoform 2 is detected in heart and skeletal muscle.
Database Links

KEGG: mmu:65973

STRING: 10090.ENSMUSP00000077273

UniGene: Mm.222206

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