Mouse Autophagy protein 5(ATG5) ELISA kit

Instructions
Code CSB-EL002293MO
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name ATG5 autophagy related 5 homolog (S. cerevisiae)
Alternative Names Atg5 ELISA kit; Apg5lAutophagy protein 5 ELISA kit; APG5-like ELISA kit
Abbreviation ATG5
Uniprot No. Q99J83
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 28 pg/mL-1800 pg/mL
Sensitivity 7 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cell Biology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse ATG5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 87
Range % 84-90
1:2 Average % 106
Range % 103-109
1:4 Average % 89
Range % 85-93
1:8 Average % 107
Range % 105-109
Recovery
The recovery of mouse ATG5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 104 100-107
EDTA plasma (n=4) 96 93-99
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
1800 2.544 2.631 2.588 2.410
900 1.977 1.904 1.941 1.763
450 1.260 1.305 1.283 1.105
225 0.773 0.798 0.786 0.608
112.5 0.546 0.584 0.565 0.387
56 0.422 0.414 0.418 0.240
28 0.290 0.306 0.298 0.120
0 0.176 0.179 0.178  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

Target Data

Function Involved in autophagic vesicle formation. Conjugation with ATG12, through a ubiquitin-like conjugating system involving ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. Involved in mitochondrial quality control after oxidative damage, and in subsequent cellular longevity. Plays a critical role in multiple aspects of lymphocyte development and is essential for both B and T lymphocyte survival and proliferation. Required for optimal processing and presentation of antigens for MHC II. Involved in the maintenance of axon morphology and membrane structures, as well as in normal adipocyte differentiation. Promotes primary ciliogenesis through removal of OFD1 from centriolar satellites and degradation of IFT20 via the autophagic pathway.
Gene References into Functions
  1. Atg5 expression in Paneth cells is essential for tissue protection against cytokine-mediated immunopathology during acute gastrointestinal infection. PMID: 29358083
  2. With increasing age, expression of the autophagy proteins ATG5 and LC3 in meniscus and articular cartilage was significantly reduced by 24 months. PMID: 28801209
  3. Investigation of the autophagy-mediated regulation of renal lipid metabolism during prolonged starvation using proximal tubular cells-specific Atg5-deficient (atg5-TSKO) mice and an in vitro serum starvation model. PMID: 28813167
  4. Atg5- and Atg7-dependent autophagy of dopaminergic neurons contributed to cellular and behavioral responses to morphine and may have implications for the future treatment of drug addiction. PMID: 28722508
  5. Intracerebral hemorrhage contributes to microglia autophagic activation through the ATG5 (and BECN1) pathway. PMID: 26732594
  6. Autophagy deficiency induced by RPE-specific deletion of Atg5 or Atg7 predisposes but does not necessarily drive the development of AMD-like phenotypes or retinal degeneration. PMID: 28465655
  7. ATG proteins ATG5 and ATG7 may be required for phagosome maturation under some conditions, but are not universally required for this process PMID: 27310610
  8. Atg5-mediated autophagy in proximal epithelial cells is a critical host-defense mechanism that prevents renal fibrosis by blocking G2/M arrest PMID: 27304991
  9. Atg5, an essential component of the autophagy machinery, is required for the development of mature natural killer (NK) cells and group 1, 2, and 3 innate innate lymphoid cell. PMID: 27210760
  10. Atg5-dependent autophagy contributes to the development of acute myeloid leukemia in an MLL-AF9-driven mouse model. PMID: 27607576
  11. Atg5 deficiency results in autophagy deficiency can lead to malignant cell transformation and resistance to Src family kinase inhibitors. PMID: 28294316
  12. Neither eosinophils nor neutrophils require ATG5-dependent autophagy for extracellular DNA trap formation. PMID: 28703297
  13. the results of this study suggest an important role of Atg5 and autophagy in maintaining the function of neutrophil extracellular traps formation in response to infection and in regulating neutrophil death PMID: 28375544
  14. Results identify the beneficial role of hypothalamic ATF4/ATG5 axis in the regulation of energy expenditure, obesity, and obesity-related metabolic disorders. PMID: 28213613
  15. this study shows that Atg5 knock-out mice have reduced disease severity in a mouse collagen induced arthritis model due to impaired T cell activation and proliferation PMID: 27624289
  16. restoration of ATG5 in the brain is sufficient to rescue Atg5-null mice from neonatal lethality. PMID: 27693508
  17. Treatment of aged mice and their derived macrophages with methyltransferase inhibitor (2)-epigallocatechin-3-gallate (EGCG) or specific DNA methyltransferase 2 (DNMT2) siRNA restored the expression of Atg5 and LC3 in vivo and in vitro. PMID: 26909551
  18. miR-30b inhibited autophagy to alleviate hepatic ischemia-reperfusion injury by decreasing the Atg12-Atg5 conjugate. PMID: 27182160
  19. These findings highlight the cell-autonomous requirement of Atg5 in the survival of adult-generated neurons. PMID: 26938300
  20. Atg5 or Atg7 deletion in the chondrocytes promotes caspase-dependent cell death and leads to mild growth retardation PMID: 26077727
  21. Data show that sequestosome 1 protein p62 deficiency increased atherosclerotic plaque burden in mice with a macrophage-specific ablation of autophagy-related 5 protein ATG5. PMID: 26732762
  22. Arg-24 of Atg16L1 is crucial for its interaction with Atg5 which has implications in the binding of the dimeric complex to Rab33B PMID: 26975471
  23. This work identifies a previously unrecognized role for alphav and the autophagy components LC3 and atg5 in regulating TLR signalling and B-cell immunity. PMID: 26965188
  24. Treg cell-specific deletion of Atg5 leads to loss of Treg cells, greater tumor resistance and development of inflammatory disorders. PMID: 26808230
  25. The results in a potent increase in odontoblastic cell differentiation, indicating the unique involvement of Atg5, autophagy and Wnt5 signaling in CS/BMP-4-induced differentiation of ES cells into odontoblast-like cells, at a relatively early stage. PMID: 26806855
  26. Deletion of Atg5 in chondrocytes promoted age-related osteoarthritis, but no difference in the development of post-traumatic osteoarthritis. PMID: 26438374
  27. the unique involvement of Atg5 in IL-1beta-induced proliferation of embryonic stem cell-derived odontoblast-like cells PMID: 25894570
  28. Data show that the autophagy-related 5 protein (Atg5)-independent autophagy is crucial for establishing pluripotency. PMID: 26502054
  29. ATG5 plays a unique role in protection against M. tuberculosis by preventing PMN-mediated immunopathology; and while Atg5 is dispensable in alveolar macrophages during M. tuberculosis infection, loss of Atg5 in PMNs can sensitize mice to M. tuberculosis PMID: 26649827
  30. Our results demonstrate that ATG5 plays important roles in intestinal tumor growth and combination of IFN-g and ATG5 deficiency or ATG5-targeted inhibition is a promising strategy for prevention and treatment of colorectal cancer . PMID: 25695667
  31. in KRas(G12D)-driven lung cancer, Atg5-regulated autophagy accelerates tumour progression; however, autophagy also represses early oncogenesis, suggesting a link between deregulated autophagy and regulatory T cell controlled anticancer immunity. PMID: 24445999
  32. Although genetic deletion of either Atg12 or Atg5 renders POMC neurons autophagy-deficient, mice lacking Atg5 in POMC neurons do not exhibit these phenotypes. PMID: 25585051
  33. Atg5 cooperated with beta-Catenin to modulate cortical NPCs differentiation and proliferation. PMID: 25109817
  34. Ulk1-dependent Atg5-independent macroautophagy is the dominant process of mitochondrial clearance from fetal definitive reticulocytes. However, Atg5-dependent macroautophagy plays a greater role in primitive and adult definitive reticulocytes. PMID: 24895007
  35. With increasing age, the expression of ATG-5 in articular cartilage decreased. PMID: 25708836
  36. Mutant mice developed mild podocyte and tubular dysfunction within 2 months, profound glomerular and tubular changes bearing close similarity to human disease by 4 months, and organ failure by 6 months. PMID: 25406339
  37. These results highlight the role of ATG5 in the dynamic regulation of ligation-induced cellular senescence and apoptosis. PMID: 25341032
  38. Together, these data demonstrate that Atg5 regulates astrocyte differentiation, with potential implications for brain disorders with autophagy deficiency. PMID: 25227738
  39. The regulation of apoptosis was enhanced in atg5(-/-) MEF cells treated with PEI. PMID: 25075440
  40. Mice with pancreas-specific disruption of Atg5 develop chronic pancreatitis. Pancreata from these mice had reduced autophagy, increased p62, and increases in endoplasmic reticulum stress and mitochondrial damage, compared with tissues from control mice. PMID: 25497209
  41. These results indicate that Brucella strains are able to invade and replicate in Atg5-deficient fibroblasts, suggesting that the canonical Atg5-dependent macroautophagic pathway is dispensable for Brucella replication. PMID: 25179110
  42. Atg5-deficient mice displayed abnormal Tbx2 expression and defects in valve development and chamber septation PMID: 24441423
  43. Atg5 deficiency-mediated autophagy increases ROS production and NF-kappaB activity in macrophages, thereby contributing to cardiac inflammation and injury PMID: 24418158
  44. We investigated the activity of autophagy-associated pathways in liver regeneration after partial hepatectomy (PHx) in liver-specific autophagy-related gene 5 (Atg5) knockout (KO) mice. PMID: 24668739
  45. Autophagy adaptor protein p62/SQSTM1 and autophagy-related gene Atg5 mediate autophagosome formation in response to Mycobacterium tuberculosis infection in dendritic cells. PMID: 24376899
  46. Deleting Atg7 or Atg5 or blocking microtubule-associated protein 1 light chain (LC)3 lipidation or ATG5-ATG12 conjugation decreases ERK phosphorylation. PMID: 24240988
  47. moderate overexpression of Atg5 in mice enhances autophagy, and Atg5 transgenic mice showed anti-ageing phenotypes, including leanness, increased insulin sensitivity and improved motor function. PMID: 23939249
  48. HuD to be an inducer of ATG5 expression and hence a critical regulator of autophagosome formation in pancreatic beta cells. PMID: 24275661
  49. Atg5 controls mucin granule accumulation in cultured colonic goblet cells.Endocytosis and Atg5 are required to generate maximal reactive oxygen species in colonic epithelial cells. PMID: 24185898
  50. Atg5 regulates the formation of MyD88 condensed structures through association with MyD88 and eventually exerts a modulatory effect on MyD88-dependent signaling PMID: 23831471
  51. These results build upon previous data demonstrating a role for ATG5 in early B cell development. PMID: 23327930
  52. the physiological role of Atg5 and Pik3c3 in quality control and development of the lens, respectively. PMID: 23479732
  53. Knockdown of ATG5 and beclin 1 blocked clinical grade IL-2-induced autophagy. PMID: 23195496
  54. Persistent activation of Nrf2 and increased hepatocyte proliferation protect against acetominophen-induced liver injury in Atg5 liver-specific knockout mice. PMID: 22491424
  55. Atg5-Atg12/Atg16L1 protein complex is required for IFNgamma-mediated host defense against murine norovirus infection. PMID: 22520467
  56. These results reveal new roles for autophagy-related molecules Atg5 and Ambra1 during early neuronal differentiation of stem/progenitor cells. PMID: 22240590
  57. Despite forming the Atg12-Atg5-Atg16L2 complex, Atg16L2 is not recruited to phagophores and is mostly present in the cytosol. PMID: 22082872
  58. ULK1 negatively regulates the kinase activity of mTORC1 and cell proliferation in a manner independent of Atg5 and TSC2 PMID: 21795849
  59. The Atg5 participates in polarized secretion of lysosomal contents into the extracellular space by directing lysosomes to fuse with the plasma membrane. PMID: 22055344
  60. The essential autophagy genes Atg5 leads to the accumulation of all detectable Ub-Ub topologies, arguing against the hypothesis that any particular Ub linkage serves as a specific autophagy signal. PMID: 21041446
  61. Inactivating Atg5 in Sindbis virus-infected neurons results in delayed clearance of viral proteins, increased accumulation of the cellular p62 adaptor protein, and increased cell death in neurons, but the levels of viral replication remain unaltered. PMID: 20159618
  62. Autophagy participated the process of immune response of anti-MTB. Atg5 were the important molecule which control the formation of autophagy when MTB infected. PMID: 19174008
  63. Mice with dendritic cell conditional deletion in Atg5 show impaired CD4-T cell priming after herpes simplex virus infection and succumbe to rapid disease PMID: 20171125
  64. Atg5 is involved in normal adipocyte differentiation, suggesting an important role of autophagy in adipogenesis. PMID: 19844159
  65. Data show that proteasome inhibition promoted autophagosome formation, stimulated autophagic flux, and upregulated expression of the autophagy-specific genes ATG5 and ATG7. PMID: 19881538
  66. Mice deficient for Atg5, which is essential for autophagosome formation, appear almost normal at birth but die within 1 day of delivery PMID: 15525940
  67. Mice deficient for Atg5 (autophagy-related 5) specifically in neural cells develop progressive deficits in motor function that are accompanied by the accumulation of cytoplasmic inclusion bodies in neurons PMID: 16625204
  68. critical role for Atg5 in multiple aspects of lymphocyte development and function PMID: 17190837
  69. Embryoid bodies derived from cells lacking the autophagy genes, atg5 or beclin 1, fail to cavitate due to persistence of cell corpses, rather than impairment of programmed cell death. PMID: 17350577
  70. Coronavirus replication does not require the autophagy gene Atg5. PMID: 17700057
  71. Overexpression of Atg5 or Atg12 resulted in Atg5-Atg12 conjugate formation and suppression of RNA-mediated signaling. PMID: 17709747
  72. Aberrant membranes and double-membrane structures accumulate in the axons of Atg5-null Purkinje cells before neuronal death. PMID: 17912025
  73. ATG5 is differentially required at discrete stages of development in distinct, but closely related, cell lineages. PMID: 18188005
  74. the inhibitory effect of Atg5 deficiency on cell death is attributable by the compensatory activation of ERK1/2 in Atg5-/- MEFs during oxidative stress-induced cell death. PMID: 18196969
  75. Atg5 expression in phagocytic cells is essential for cellular immunity to intracellular pathogens in vivo. PMID: 18996346
  76. Increased T cell expression of Atg5 may contribute to inflammatory demyelination in multiple sclerosis. PMID: 19066443
  77. One role for the autophagy pathway in Crohn disease pathogenesis is through selective effects on the biology and specialized properties of Paneth cells. PMID: 19139628
  78. knockout mice display alteration in T cell receptor selection and self-tolerance induction PMID: 19246181
  79. Deletion of the essential autophagy genes Atg5 or Atg7 in T cells resulted in decreased thymocyte and peripheral T cell numbers, and Atg5-deficient T cells had a decrease in cell survival. PMID: 19276668
  80. expression is elevated in mouse embryo and neuroblastoma cells after MDMA treatment PMID: 19466606
  81. upregulation of Atg5 is required for the oncogenic H-ras-induced autophagic cell death in normal fibroblasts and that activation of Rac1/MKK7/JNK-signaling pathway leads to upregulation of Atg5 in response to oncogenic H-ras PMID: 19783847
  82. macroautophagy can occur through at least two different pathways: an Atg5/Atg7-dependent conventional pathway and an Atg5/Atg7-independent alternative pathway PMID: 19794493

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Subcellular Location Cytoplasm, Preautophagosomal structure membrane, Peripheral membrane protein
Protein Families ATG5 family
Tissue Specificity Ubiquitous.
Database Links

KEGG: mmu:11793

STRING: 10090.ENSMUSP00000044769

UniGene: Mm.22264

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