Mouse Bcl-2 associated X protein, Bax ELISA Kit

Code CSB-E17114m
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. Q07813
Lead Time 3-5 working days
Abbreviation BAX
Protein Biological Process 1 Apoptosis/Autophagy
Target Name BCL2-associated X protein
Alias BCL2L4, apoptosis regulator BAX
Species Mus musculus (Mouse)
Protein Biological Process 3 Apoptosis
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 7.8 pg/mL-500 pg/mL
Sensitivity 1.95 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cell Biology
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This protein belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene.
HGNC 959
RGD 2192
MGI 99702
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse Bax in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:1 Average % 108    
Range % 96-112    
1:2 Average % 100    
Range % 93-105    
1:4 Average % 93    
Range % 86-98    
1:8 Average % 94    
Range % 88-99    
Recovery
The recovery of mouse Bax spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 87 81-92    
EDTA plasma (n=4) 100 93-106    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
pg/ml OD1 OD2 Average Corrected    
500 2.127 2.099 2.113 1.930    
250 1.448 1.387 1.418 1.235    
125 0.975 0.932 0.954 0.771    
62.5 0.663 0.642 0.653 0.470    
31.2 0.449 0.456 0.453 0.270    
15.6 0.348 0.341 0.345 0.162    
7.8 0.283 0.275 0.279 0.096    
0 0.185 0.181 0.183      
References
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Function Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis. BAX deficiency leads to lymphoid hyperplasia and male sterility, because of the cessation of sperm production.
Subcellular Location Isoform Alpha: Mitochondrion outer membrane, Single-pass membrane protein, Cytoplasm
Protein Families Bcl-2 family
Tissue Specificity Expressed in a wide variety of tissues.
Database Links

KEGG: mmu:12028

STRING: 10090.ENSMUSP00000033093

UniGene: Mm.19904

Pathway p53 signaling pathway
Apoptosis
Protein processing in endoplasmic reticulum
Necroptosis
AGE-RAGE signaling pathway in diabetic complications
Neurotrophin signaling pathway

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