Mouse Bcl-2 associated X protein, Bax ELISA Kit

Instructions
Code CSB-E17114m
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name BCL2-associated X protein
Alternative Names BaxApoptosis regulator BAX ELISA kit
Abbreviation BAX
Uniprot No. Q07813
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 7.8 pg/mL-500 pg/mL
Sensitivity 1.95 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cell Biology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse Bax in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:1 Average % 108    
Range % 96-112    
1:2 Average % 100    
Range % 93-105    
1:4 Average % 93    
Range % 86-98    
1:8 Average % 94    
Range % 88-99    
Recovery
The recovery of mouse Bax spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 87 81-92    
EDTA plasma (n=4) 100 93-106    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
pg/ml OD1 OD2 Average Corrected    
500 2.127 2.099 2.113 1.930    
250 1.448 1.387 1.418 1.235    
125 0.975 0.932 0.954 0.771    
62.5 0.663 0.642 0.653 0.470    
31.2 0.449 0.456 0.453 0.270    
15.6 0.348 0.341 0.345 0.162    
7.8 0.283 0.275 0.279 0.096    
0 0.185 0.181 0.183      
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis. BAX deficiency leads to lymphoid hyperplasia and male sterility, because of the cessation of sperm production.
Gene References into Functions
  1. T-2 toxin appears to activate specific intracellular death-related pathways leading to Bax-dependent caspase-3 activation and the induction of apoptosis in Leydig cells. PMID: 28697680
  2. The data revealed that autophagy is an important regulator of osteoblastic apoptosis through its interaction with Bax/Bcl2, and maintains the osteoblastic function of MC3T3E1 cells following GC exposure. In addition, these results indicated that the suppression of autophagy in OBs under chronic GC therapy may increase the prevalence of GCinduced osteoporosis and fragility fractures. PMID: 29207032
  3. although all CR subtypes undergo cell death, septum, but not hem, CRs die in a Bax-dependent manner. Bax-inactivated rescued septum-CRs maintain immature electrophysiological properties PMID: 28009284
  4. An autoinhibited dimeric form of BAX regulates the cytosolic BAX activation pathway. PMID: 27425408
  5. Study found that emphysema occurred in ku70(-/-) mice at the age of three-months old, and that Bax deficiency was able to suppress it. These results suggest that Bax-mediated apoptosis induces emphysema in ku70(-/-) mice. PMID: 27302174
  6. The polyglutamine embedded in the ER membrane was observed at the same time as Bax insertion. These results demonstrated that the ER membrane may be a target of polyglutamine, which triggers cell death through Bax. PMID: 27079237
  7. Its gene expression is up-regulated by hyperoxia. PMID: 26718146
  8. These results suggest that Bax mediates beta-cell apoptosis in Pdx1-deficient diabetes. PMID: 27137932
  9. Bim and Puma overlapped apoptosis only partially during physiological apoptotic stage and they were present in non-apoptotic parts of the follicles. PMID: 26179069
  10. This study demonstated that Bax KO mice show Hyperactivity and depression. PMID: 26363094
  11. The influence of chronic ethanol consumption on the expression of the Bdnf, Bax, Bcl-xL, and CASP3 genes was studied in the brain structures of B6-1473C (C/C) and B6-1473G (G/G) mice that had been obtained on the base of the C57BL/6 strain. PMID: 27239851
  12. These data indicate that the rapid and robust microglial activation and PIF expression observed in situ following acute EtOH exposure is largely driven indirectly by BAX-dependent apoptotic cell death, rather than directly by the EtOH. PMID: 25856413
  13. despite its role in Bax inhibition, Bcl-xL also primes mitochondria to permeabilization and cytochrome c release. PMID: 25862283
  14. The Ca(2+) channel inhibitor 2-APB reverses beta-amyloid-induced long term potentiation deficit in hippocampus by blocking BAX and caspase-3 hyperactivation. PMID: 25521332
  15. Although theanine had no significant effect on the doxorubicin induced elevation in the expression level of mRNA for Fas in the liver, the increase in hepatic expression of Bax was significantly attenuated by theanine. PMID: 25680506
  16. Bax deficiency appeared to delay the development of emphysema. This study suggests that enhanced Bax activity exacerbates the negative impact of Ku70 deletion PMID: 25811803
  17. Overall, our findings provide evidence for a novel stress-response, which is regulated by a non-canonical action of Bax on the nuclear envelope . PMID: 25482068
  18. Annona muricata has antiproliferative effects on BPH-1 cells and reduces prostate size, possibly through apoptosis PMID: 25411208
  19. Bax localizes to the mitochondrial outer membrane via alternate mechanisms, either constitutively via an interaction with VDAC2 or after activation via interaction with Bcl-2 family proteins. PMID: 25146925
  20. Data show that p38a directly induces myofiber death through a mitochondrial-dependent pathway involving direct phosphorylation and activation of the pro-death Bcl-2 family member Bax. PMID: 24876160
  21. Mitochondrial shape governs BAX-induced membrane permeabilization and apoptosis via Mfn1. PMID: 25482509
  22. these data suggested that Bax participates in the efficient flux of Ca2+ between cytosol and the ER and that inhibition of this activity may be associated with neuroprotection and reduced cytosolic Ca2+ overloading during excitotoxic injury. PMID: 25632145
  23. Binding to membranes is regulated by cleavage of Bid to truncated Bid (tBid), by conformation changes in tBid and Bax, and by interactions with other proteins. PMID: 24901048
  24. Data indicate that the conformational changes in the pro-apoptotic protein Bax coincide with the commitment to apoptosis. PMID: 25315775
  25. BAX has a role in cell survival through mitochondrial fusion PMID: 25111896
  26. Suggest that S-palmitoylation of BAX at Cys126 is a key regulatory process of BAX-mediated apoptosis. PMID: 24525733
  27. It regulates Parkinson's disease development. PMID: 24325796
  28. a missense mutation in the C-terminal transmembrane domain of proapoptotic BAX (G179E) was found, which abrogated BAX anchoring to mitochondria and blocked ABT-199-induced apoptosis both in vitro and in vivo PMID: 24786774
  29. Activation of Bax was observed in all three models of retinitis pigmentosa and leads to neurodamage by localization at the mitochondrion. PMID: 24825107
  30. Lipopolysaccharide significantly decreased the expression of Bcl-2 and increased the expression of Bax and caspase-3. PMID: 24145669
  31. Polyglutamine oligomers augmented at the endoplasmic reticulum (ER) induce insertion of Bax into the ER membrane, thereby activating caspase-7. PMID: 24388981
  32. This study demonistrated that Bax null mutant mice at E17 had 132% of the WT number of TG cells (p < 0.001), although the numbers returned to WT levels by newborn mice. PMID: 23614607
  33. Bax expression has a neuroprotective effect on cell hypoxia. PMID: 23713735
  34. Loss of BOK does not exacerbate the abnormalities caused by loss of BAX. PMID: 23744350
  35. Bax expression was sufficient to restore NPR. PMID: 23475110
  36. These results suggest that the cell replacement is necessary for the normal olfactory learning behavior, and the chronic perturbation of cell replacement may result in the imbalance of neural circuits driving enhancement of olfactory learning ability. PMID: 23685130
  37. Constitutive activation of proapoptotic Bax is a mechanism for rapid cell death preserved in medulloblastoma. PMID: 24227720
  38. Data indicate that Mtch2 accelerated conformational change in membrane-bound tBid (Bid) enables it to activate Bax. PMID: 23744079
  39. Bax and Bak mediate non-redundant functions during murine cytomegalovirus infection. PMID: 23468630
  40. Myeloid-derived suppressor cells use down-regulation of IRF8 to alter Bax and Bcl-xL expression to deregulate the Fas-mediated apoptosis pathway to evade elimination by host CTLs PMID: 23677993
  41. Bax-dependent apoptosis is an essential force in postnatal neurogenesis in the cerebellum, and that Bax presents an obstacle that is overcome in medulloblastoma pathogenesis. PMID: 22710714
  42. NPM-Bax interaction enhances mitochondrial Bax accumulation, organelle injury, and cell death. PMID: 23459946
  43. these data suggest a change in strategy with anxiety and defensive behaviors in Bax-/- mice. PMID: 23142367
  44. identify a new finding that HG-induced podocyte apoptosis is mediated by calcineurin/NFAT2/Bax signaling pathway, which may present a promising target for therapeutic intervention PMID: 23340267
  45. Bax is normally in a dynamic equilibrium between cytosol and mitochondria, enabling fluctuations in survival signals to finely adjust apoptotic sensitivity PMID: 23375500
  46. This study demonistrated that Bax mutant mice exhibit conspicuous changes in the organization of the M1 ipRGC mosaic. PMID: 23395376
  47. Study describes crystal structures that clarify the structural transitions of Bax toward apoptosis. PMID: 23374347
  48. Decreased IRS2 expression and increased Bax expression may play an important role in the glucotoxicity in mouse islet cells. PMID: 19620044
  49. HDAC6 siRNA down-regulated the expressions of HDAC6 and bcl-2, and up-regulated the expression of bax in xenografted human laryngeal carcinoma cells. PMID: 22932141
  50. Germ cell death during testicular germ cell-induced experimental autoimmune orchitis is mediated by the Fas/Fas-L and Bax/Bcl-2 systems during the active inflammation stage. PMID: 22403281

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Subcellular Location Isoform Alpha: Mitochondrion outer membrane, Single-pass membrane protein, Cytoplasm
Protein Families Bcl-2 family
Tissue Specificity Expressed in a wide variety of tissues.
Database Links

KEGG: mmu:12028

STRING: 10090.ENSMUSP00000033093

UniGene: Mm.19904

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