Mouse Beta-2 adrenergic receptor(ADRB2) ELISA kit

Instructions
Code CSB-EL001392MO
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name adrenergic, beta-2-, receptor, surface
Alternative Names Adrb2 ELISA kit; Adrb2rBeta-2 adrenergic receptor ELISA kit; Beta-2 adrenoreceptor ELISA kit; Beta-2 adrenoceptor ELISA kit
Abbreviation ADRB2
Uniprot No. P18762
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 18.75 pg/mL-1200 pg/mL
Sensitivity 4.69 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse ADRB2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %90
Range %85-94
1:2Average %98
Range %94-102
1:4Average %102
Range %94-108
1:8Average %94
Range %87-96
Recovery
The recovery of mouse ADRB2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9794-103
EDTA plasma (n=4)9389-96
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
12002.902 2.855 2.879 2.737
6002.447 2.364 2.406 2.264
3001.884 1.900 1.892 1.750
1501.328 1.268 1.298 1.156
750.695 0.740 0.718 0.576
37.50.499 0.480 0.490 0.348
18.750.302 0.297 0.300 0.158
00.137 0.147 0.142
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

Target Data

Function Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. The beta-2-adrenergic receptor binds epinephrine with an approximately 30-fold greater affinity than it does norepinephrine.
Gene References into Functions
  1. we identified the beta2 adrenergic receptor (ADRB2) as a downstream target for PHD3 in skeletal muscle PMID: 28939592
  2. Here, the mechanism by which norepinephrine (NE)regulates prostate cancer DTCs in the marrow is explored. NE directly stimulated prostate cancer cell proliferation through beta2-adrenergic receptors (ADRB2).. NE also altered prostate cancer proliferation in the marrow niche by indirectly downregulating the secretion of the dormancy inducing molecule growth arrest specific-6 (GAS6) expressed by osteoblasts. PMID: 28814453
  3. Adrenergic Receptor beta-2 signaling in the airway epithelium promotes eosinophilic inflammation, mucous metaplasia, and airway contractility. PMID: 29073113
  4. this review discusses beta2-adrenoceptor function in asthma PMID: 28950943
  5. the present study demonstrated that cardiac contractility/relaxation and heart rate is increased in beta1AR TG and beta2AR TG mice, and indicated that this increase may be related to the overexpression of G proteins and Gproteinassociated proteins. PMID: 28487987
  6. S1928A KI mice failed to induce long-term potentiation in response to prolonged theta-tetanus (PTT-LTP), a form of synaptic plasticity that requires Cav1.2 and enhancement of its activity by the beta2-adrenergic receptor (beta2AR)-cAMP-PKA cascade. PMID: 28119465
  7. activation of beta2AR decreased cerebral amyloid plaques through the up-regulation of alpha-secretase activity and by decreasing the phosphorylation of APP at Thr668 PMID: 27838449
  8. Together, these data indicate that high-fat diet promotes phosphorylation of the beta2 AR, contributing to impairment of cardiac contractile reserve before cardiac structural and functional remodelling, suggesting that early intervention in the insulin-adrenergic signalling network might be effective in prevention of cardiac complications in diabetes PMID: 27983752
  9. beta2-AR deletion is associated to selective hepatic insulin resistance and preserved skeletal muscle insulin sensitivity. PMID: 28684080
  10. The diurnal variation of the humoral immune response was dependent on beta2AR-mediated neural signals and was diminished when lymphocyte recirculation through lymph nodes was stopped. PMID: 27799619
  11. knocking out of the beta1/2 receptor significantly diminished the ST25 acupuncture-induced inhibition of gastric motility and jejunal motility without significantly altering the enhancement of colonic motility induced by acupuncture at ST25. PMID: 27978539
  12. The authors show that phosphorylation of S1928 displaces the beta-2 adrenergic receptor from Cav1.2 upon beta-adrenergic stimulation rendering Cav1.2 refractory for several minutes from further beta-adrenergic stimulation. PMID: 27103070
  13. The Galphas and Galphaq peptides adopt different orientations in beta2-AR and V1AR, respectively. The beta2-AR/Galphas peptide interface is dominated by electrostatic interactions, whereas the V1AR/Galphaq peptide interactions are predominantly hydrophobic. PMID: 27330078
  14. Thus the beta2AR acts as a double-edged sword: increasing TAS2R14 cell surface expression, but when activated by beta-agonist, partially offsetting the expression phenotype by direct receptor:receptor desensitization of TAS2R14 function. PMID: 27342779
  15. Data show that inhibitory (Galphai2) and stimulatory (GalphasL) G-protein subunits produced minor atrophic and hypertrophic changes in muscle mass, and Galphai2 over-expression prevented AAV:beta2-adrenoceptor (beta2-AR) mediated hypertrophy. PMID: 26972746
  16. Age-related increase in hepatic triglyceride levels is reduced upon beta2-AR ablation. beta2-AR ablation improves glucose tolerance in old mice. PMID: 26952573
  17. Immune cell-expressed beta2AR plays an essential role in regulating the early inflammatory repair response to acute myocardial injury by facilitating cardiac leukocyte infiltration. PMID: 27364164
  18. In epileptic seizures mouse model, Pkdl deficiency leads to the loss of beta2AR on neuronal cilia, reduction in cAMP levels in the central nervous system, and reduction in the activation of cAMP response element-binding protein. PMID: 26612203
  19. Beta2AR overexpression enhances endothelial progenitor cells (EPC) functions in vitro and enhances the vascular repair abilities of EPCs in vivo via the beta2AR/Akt/eNOS pathway. PMID: 27194135
  20. Data show that beta2-AR plays an important role in prostate cancer metastasis formation. PMID: 26058426
  21. Inhibition of type 5 phosphodiesterase counteracts beta2-adrenergic signalling in beating cardiomyocytes. PMID: 25852085
  22. Housing temperature-induced stress drives therapeutic resistance in murine tumor models through beta2-adrenergic receptor activation. PMID: 25756236
  23. Quantification of beta adrenergic receptor subtypes in beta-arrestin knockout mouse airways. PMID: 25658948
  24. our results demonstrate that the beta2-adrenergic receptor is a key regulator of hepatic autophagy, and that the beta-blocker propranolol can independently induce a late block in autophagy. PMID: 24950230
  25. promotes the M2 macrophage phenotype in mouse models of endotoxemia and LPS-induced acute lung injury PMID: 24642449
  26. Lack of beta2 -adrenoceptors aggravates heart failure-induced skeletal muscle myopathy. PMID: 24629015
  27. This study demonstrated that TNFalpha plays a role in insulin resistance in beta2KO mice. PMID: 25138272
  28. Our results suggest that the beta2-adrenoceptor contributes to the regulation of the initial phases of muscle regeneration PMID: 24938737
  29. beta2AR-mediated signals inhibited lymph node egress of antigen-primed T cells and reduced their recruitment into peripheral tissues. PMID: 25422496
  30. role of beta-ARs in early muscle regeneration PMID: 25000590
  31. Adrenergic beta 2 receptor deficiency ameliorates tau pathology in a mouse model of tauopathies. PMID: 24626633
  32. Gene therapy or pharmacological means of increasing Galphas (or its coupling efficiency to beta2AR) thus represent an interface for development of novel therapeutic agents for improvement of beta-agonist therapy. PMID: 25260754
  33. Data indicate that adrenergic receptor beta2 activation modulates dendritic cells (DCs) function resulting in skewing towards anti-inflammatory T cell phenotypes. PMID: 24465481
  34. Beta-2-Adrenergic and M2-muscarinic receptors decrease basal t-tubular L-type calcium channel activity and suppress ventricular contractility in heart failure. PMID: 24389135
  35. These data indicate that increased insulin receptor signaling, may directly impair beta2-adrenergic receptor regulated cardiac contractility. PMID: 24677713
  36. specific interactions between the ligand and the Tyr-308 residue of beta2-AR stabilize receptor conformations favoring the receptor-Gs protein coupling and subsequently result in Gs-biased agonism. PMID: 24831005
  37. the cardio-protective effect of S-nitroso-N-acetylcysteine treatment is primarily through its anti-oxidant role and is associated with beta-ARs overexpression and beta-AR-SNO via an anti-apoptotic pathway PMID: 24333561
  38. The results of this study suggest that loss of beta2AR signalling results in a neuroprotective phenotype in part due to decreased NF-kappaB signalling, decreased inflammation and decreased apoptotic signalling in the brain. PMID: 22867428
  39. This study report that both beta1- and beta2-ARs are expressed in a majority of GABAergic interneurons in the medial prefrontal cortex of mice. PMID: 24215978
  40. Homotypic and heterotypic adhesion induced by odorant receptors and the beta2-adrenergic receptor. PMID: 24312457
  41. ADRB2 stimulation has caused a marked increase in the autophagy-targeted lipid droplets (LD) for lysosomal degradation, which is dependent on the LD-associated RAB7. PMID: 23708524
  42. Data indicate that adrenergic receptor beta2 knockout (ARbeta2KO) mice preserved cold- and diet-induced adaptive thermogenesis but disrupts glucose homeostasis. PMID: 24868110
  43. Disruption of gravin's scaffold mediated signaling is able to increase baseline cardiac function as well as to augment contractility in response to acute beta-AR stimulation. PMID: 24058627
  44. Data indicate that ischemic injury coupled to G protein-coupled receptor beta1/2 adrenergic receptors activation required GRK2 (GPCR kinase 2) activation. PMID: 24170934
  45. Deletion of the beta2-adrenergic receptor prevents the development of cardiomyopathy in mice. PMID: 23920331
  46. We demonstrate that beta2-adrenergic receptor-dependent activation of proinflammatory macrophages is critical for epinephrine-mediated IL-6 production in wound healing. PMID: 24121404
  47. the beta2-AR-HIF-1alpha axis regulates stress-induced pancreatic tumor growth and angiogenesis. PMID: 23745588
  48. GRK2 blockade with betaARKct is essential for cardiac beta2-adrenergic receptor signaling towards increased contractility. PMID: 23984976
  49. beta2-adrenergic receptor knockout mice exhibit A diabetic retinopathy phenotype. PMID: 23894672
  50. expression and function of beta(3)-adrenoceptors and beta(2)-adrenoceptors in mouse uterus PMID: 23219791
  51. observed that beta2-AR KO mice showed cardiac atrophy at rest PMID: 23090606
  52. Pharmacological blockade of a beta(2)AR-beta-arrestin-1 signaling cascade prevents the accumulation of DNA damage in a behavioral stress model. PMID: 23287463
  53. beta2 adrenergic receptor, protein kinase A (PKA) and c-Jun N-terminal kinase (JNK) signaling pathways mediate tau pathology in Alzheimer disease models PMID: 23430246
  54. The present study provides the first evidence that endothelial stem cells express a functional beta2AR and stimulation results in EPCs proliferation, migration, and differentiation, enhancing their angiogenic ability. PMID: 23418295
  55. the present findings provide evidence for the contribution of beta2-adrenergic receptor to skeletal muscle phenotype, metabolism and endurance capacity in mice. PMID: 22913394
  56. a novel function of palmitoylation in shaping subcellular cAMP-PKA signaling in cardiomyocytes via modulating the recruitment of beta arrestin 2-PDE4D complexes to the agonist-stimulated beta(2)AR PMID: 22912718
  57. In muscararinic M2 receptor knockout mice,heart function is not much affected; as possible mechanisms of this normal cardiac function, decreases of gene expression and receptor binding of beta1- and beta2-adrenoceptors should be considered PMID: 23093370
  58. beta-Adrenergic stimulation promotes expansion and survival of cardiac progenitor cells through ADRB2. PMID: 23243208
  59. Mechanisms of delayed inotropic response of mouse atria to activation of beta(2)-adrenoreceptors. PMID: 23129275
  60. Results suggest that the effects of zinc-alpha2-glycoprotein (ZAG) on body weight and insulin sensitivity in ob/ob mice are manifested through beta-adrenoreceptor (beta-AR) beta-3AR or possibly a beta2-AR. PMID: 22227600
  61. A potential cooperative role for beta(1) and beta(2) adrenergic receptors in stress-induced reinstatement. PMID: 22593095
  62. beta1- and beta2-adrenergic signaling exert opposite effects on bone- beta1 exerting a predominant anabolic stimulus in response to mechanical stimulation and during growth, whereas beta2-adrenergic receptor signaling mainly regulates bone resorption during aging. PMID: 22407956
  63. Intracellular signaling and cell mechanics using isolated human airway smooth muscle, mouse tracheal responses, and human bronchial responses to characterize TAS2R relaxation in the context of beta(2)AR desensitization. PMID: 22683571
  64. beta2AR antagonists and beta2AR gene deletion both promote skin wound repair processes PMID: 22495178
  65. Upon beta(2)AR activation, both betaArrestin2 and p115RhoGEF translocate to the plasma membrane, with concomitant activation of RhoA and formation of focal adhesions and stress fibers. PMID: 22500016
  66. Data implicate a functional interaction between parathyroid hormone type 1 receptor (PTHR) and beta(2)-adrenergic receptor (beta(2)AR) controls bone formation and parathyroid hormone (PTH) anabolism. PMID: 22538810
  67. Data show that overexpression of beta1- and beta2-adrenergic receptors in hepatocytes from young mice and rats increased cellular lipid content. PMID: 22457517
  68. a model in which beta2 receptor clustering is influenced by the actin cytoskeleton, but it does not rely on lipid raft integrity PMID: 22442147
  69. reduced beta(2)AR expression contributes to the age-related decline of glucose tolerance in mice. PMID: 22315324
  70. Signaling by the beta2-adrenergic receptor is redundant with signaling by dopamine at the D5-dopaminergic receptor, hence stress-responsive neurotransmitters norepinephrine and epinephrine promote and enhance fear memory consolidation via phospholipase C. PMID: 22323706
  71. beta-Adrenergic receptor-PI3K signaling crosstalk in mouse heart PMID: 22028912
  72. beta-arrestin2 mediates beta-2 adrenergic receptor signaling inducing prostate cancer cell progression PMID: 21833475
  73. The results of this study provided substantial and unexpected evidence that signaling by Gi/o-coupled beta2-adrenergic receptors in the hippocampus mediates xamoterol-induced impairment of memory retrieval. PMID: 21878527
  74. Confinement of beta 1- and beta 2-adrenergic receptors in the plasma membrane of cardiomyocytes is mediated by selective interactions with PDZ domain and A-kinase anchoring proteins PMID: 21680711
  75. Stress- and glucocorticoid-induced impairments of retrieval depend on the activation of beta2 (but not beta1)-adrenergic receptors. PMID: 21976502
  76. High (stress) levels of epinephrine directly compromise the activity of neonatal mouse skin fibroblasts through the activation of beta1-, beta2- and beta3-adrenoceptors, but not through alpha1- and alpha2-adrenoceptors. PMID: 21366703
  77. renin expression was found to be significantly lower at all developmental stages in the kidneys of adrenergic beta(1)/beta(2) knockout mice PMID: 21389089
  78. Stress-induced reinstatement requires noradrenergic signaling through beta-2 adrenergic receptors. PMID: 20613718
  79. although b1-adrenoceptors are the predominant b-adrenoceptor in mouse astrocytes, activation of b2- and b3-adrenoceptors increases glucose uptake PMID: 21138422
  80. beta2-adrenergic receptors inhibit the expression of collagen type II in growth plate chondrocytes by stimulating the AP-1 factor Jun-B. PMID: 21177286
  81. Both pharmacological and genetic beta2AR blockade in mice significantly reduced the bone catabolic effect of high-dose prednisolone in vivo. PMID: 21266510
  82. Data show that PI3Kgamma inhibits protein phosphatase 2A at the beta-adrenergic receptor complex, altering G protein coupling. PMID: 21419339
  83. In adrenergic beta2 knockout mice, which have perturbed retinal waves, retinofugal single axon arbors are well ramified but enlarged, particularly in the superior colliculus. PMID: 21368050
  84. The decrease in beta(2)-AR may account for additional relaxation impairment, given that there is no enhancement over nontransgenic after pertussis toxin, despite AC5 overexpression. PMID: 21131397
  85. Suggest that the acute actions of short-acting beta(2)-adrenoceptor agonists on force production in mammalian skeletal muscles are mediated through the beta(2)-adrenoceptor, whereas those of long-acting beta(2)-adrenoceptor agonists are not. PMID: 20864554
  86. 5-HT-R splice variants and beta-ARs are differentially regulated in the embryonic telencephalon PMID: 20805681
  87. Abnormal beta(2)-AR activation at early pregnancy provided a molecular clue in explaining how maternal stress at early stages could adversely affect the pregnancy outcome. PMID: 21148315
  88. negative inotropy and heart hypertrophy occus in total adrenergic beta receptor deficiency PMID: 20406048
  89. degradation of beta2-AR and impairment of Hcy metabolism is implicated in diabetic cardiomyopathy. PMID: 20836991
  90. Data show that both the mRNA and protein levels of beta2- and beta3-adrenergic receptors were up-regulated following cAMP/PKA-mediated adipogenesis of mouse bone marrow derived mesenchymal stem cells. PMID: 20363288
  91. Activation of ss2 adrenergic receptor reverses both phosphoinositide 3-kinase and phosphodiesterase 4-mediated increases in myocardial contractility. PMID: 20705779
  92. Study reveals that non-neurotransmitter Abeta has a binding capacity to beta(2)AR and induces PKA-dependent hyperactivity in AMPA receptors. PMID: 20395454
  93. the unique signaling effects of enhanced beta(2)AR and AC5, which have the potential to afford benefit in heart failure, failed to salvage ventricular function in beta(1)AR-mediated cardiomyopathy. PMID: 20443853
  94. beta-Adrenergic receptors transmit sympathetic signals to the ion secretory elements of the colonic epithelial cells stimulating a transient Cl ion secretion and a sustained K ion secretion. PMID: 20413718
  95. results suggest that acute stress induces abnormal activation of beta(2)-AR which subsequently enhances Abeta peptides (the main neuropathological hallmarks of Alzheimer's disease (AD)) production possibly resulting in the onset of AD PMID: 20059989
  96. Both beta2- and beta3-AR are involved in MSC osteogenesis, with beta2-AR being dominant. PMID: 20138019
  97. study found that,in cardiomyocytes from healthy adult rats & mice, spatially confined beta2AR-induced cAMP signals are localized exclusively to the deep transverse tubules, whereas functional beta1ARs are distributed across the entire cell surface PMID: 20185685
  98. [beta]2-AR play a significant role in regulating LPS-mediated mortality and inflammation. PMID: 19333138
  99. Findings suggest that the cAMP increase induced by stimulation of beta(2)-AR in skeletal muscles is possibly the mechanism by which catecholamines suppress atrogin-1 and the UPS mediated via phosphorylation of Akt and thus inactivation of Foxo3. PMID: 19837877
  100. of arrestin3 to the beta2-adrenergic receptor orchestrates the sequestration of Gq-coupled receptor-induced ERK to the cytosol through direct binding of ERK to arrestin. PMID: 19926878

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Subcellular Location Cell membrane, Multi-pass membrane protein, Early endosome
Protein Families G-protein coupled receptor 1 family, Adrenergic receptor subfamily, ADRB2 sub-subfamily
Database Links

KEGG: mmu:11555

STRING: 10090.ENSMUSP00000062256

UniGene: Mm.5598

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