Mouse Beta-2 adrenergic receptor(ADRB2) ELISA kit

Code CSB-EL001392MO
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application

Product Details

Target Name adrenergic, beta-2-, receptor, surface
Alternative Names ADRB2R, ADRBR, B2AR, BAR, BETA2AR, beta-2 adrenergic receptor|beta-2 adrenoceptor|catecholamine receptor
Abbreviation ADRB2
Uniprot No. P18762
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 18.75 pg/mL-1200 pg/mL
Sensitivity 4.69 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of mouse ADRB2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
1:1Average %90
Range %85-94
1:2Average %98
Range %94-102
1:4Average %102
Range %94-108
1:8Average %94
Range %87-96
The recovery of mouse ADRB2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9794-103
EDTA plasma (n=4)9389-96
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
12002.902 2.855 2.879 2.737
6002.447 2.364 2.406 2.264
3001.884 1.900 1.892 1.750
1501.328 1.268 1.298 1.156
750.695 0.740 0.718 0.576
37.50.499 0.480 0.490 0.348
18.750.302 0.297 0.300 0.158
00.137 0.147 0.142
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days
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Target Data

Function Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. The beta-2-adrenergic receptor binds epinephrine with an approximately 30-fold greater affinity than it does norepinephrine.
Subcellular Location Cell membrane, Multi-pass membrane protein, Early endosome
Protein Families G-protein coupled receptor 1 family, Adrenergic receptor subfamily, ADRB2 sub-subfamily
Database Links

KEGG: mmu:11555

STRING: 10090.ENSMUSP00000062256

UniGene: Mm.5598

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