Mouse Beta-2 adrenergic receptor(ADRB2) ELISA kit

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Target Data

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Product Details

Target Name

adrenergic, beta-2-, receptor, surface

Alternative Names

ADRB2R, ADRBR, B2AR, BAR, BETA2AR, beta-2 adrenergic receptor|beta-2 adrenoceptor|catecholamine receptor

Abbreviation

ADRB2

Uniprot No.

P18762

Species

Mus musculus (Mouse)

Sample Types

serum, plasma, tissue homogenates

Detection Range

18.75 pg/mL-1200 pg/mL

Sensitivity

4.69 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse ADRB2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	85-94
1:2	Average %	98
	Range %	94-102
1:4	Average %	102
	Range %	94-108
1:8	Average %	94
	Range %	87-96

Recovery

The recovery of mouse ADRB2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	94-103
EDTA plasma (n=4)	93	89-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1200	2.902	2.855	2.879	2.737
600	2.447	2.364	2.406	2.264
300	1.884	1.900	1.892	1.750
150	1.328	1.268	1.298	1.156
75	0.695	0.740	0.718	0.576
37.5	0.499	0.480	0.490	0.348
18.75	0.302	0.297	0.300	0.158
0	0.137	0.147	0.142

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

7-14 working days

Still Have Questions?

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Target Data

Function	Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. The beta-2-adrenergic receptor binds epinephrine with an approximately 30-fold greater affinity than it does norepinephrine.
Subcellular Location	Cell membrane, Multi-pass membrane protein, Early endosome
Protein Families	G-protein coupled receptor 1 family, Adrenergic receptor subfamily, ADRB2 sub-subfamily
Database Links	KEGG: mmu:11555 STRING: 10090.ENSMUSP00000062256 UniGene: Mm.5598

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Check Code：

Code	CSB-EL001392MO
Size	96T,5×96T,10×96T
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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.