Mouse C-C motif chemokine 25(CCL25) ELISA kit

Code CSB-EL004789MO
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. O35903
Lead Time 5-7 working days
Protein Biological Process 2 chemokine
Abbreviation CCL25
Protein Biological Process 1 Immunity
Target Name C-C motif chemokine 25(CCL25)
Alias Ckb15, MGC150327, SCYA25, TECK, Ck beta-15|TECKvar|chemokine TECK|small inducible cytokine A25|small inducible cytokine subfamily A (Cys-Cys), member 25|thymus expressed chemokine
Species Mus musculus (Mouse)
Protein Biological Process 3 Chemotaxis
Sample Types serum, plasma, tissue homogenates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.8 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This gene belongs to the subfamily of small cytokine CC genes. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene displays chemotactic activity for dendritic cells, thymocytes, and activated macrophages but is inactive on peripheral blood lymphocytes and neutrophils. The product of this gene binds to chemokine receptor CCR9.
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse CCL25 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 101
Range % 98-105
1:2 Average % 99
Range % 96-103
1:4 Average % 89
Range % 85-94
1:8 Average % 97
Range % 92-103
Recovery
The recovery of mouse CCL25 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 95 91-98
EDTA plasma (n=4) 88 83-92
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
2000 2.357 2.366 2.362 2.231
1000 1.825 1.812 1.819 1.688
500 1.260 1.366 1.313 1.182
250 0.751 0.745 0.748 0.617
125 0.478 0.498 0.488 0.357
62.5 0.313 0.322 0.318 0.187
31.25 0.217 0.221 0.219 0.088
0 0.128 0.133 0.131  
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Function Potentially involved in T-cell development. Recombinant protein shows chemotactic activity on thymocytes, macrophages, THP-1 cells, and dendritics cells but is inactive on peripheral blood lymphocytes and neutrophils. Binds to CCR9. Binds to atypical chemokine receptor ACKR4 and mediates the recruitment of beta-arrestin (ARRB1/2) to ACKR4.
Subcellular Location Secreted
Protein Families Intercrine beta (chemokine CC) family
Tissue Specificity Specifically expressed by thymic dendritic cells. High levels in thymus and small intestine.
Database Links

KEGG: mmu:20300

STRING: 10090.ENSMUSP00000024004

UniGene: Mm.7275

Pathway Chemokine signaling pathway

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