Mouse Calcipressin-1(RCAN1) ELISA kit

Unavailable
Code CSB-EL019500MO
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. Q9JHG6
Lead Time 7-14 working days
Abbreviation RCAN1
Target Name regulator of calcineurin 1
Alias ADAPT78, CSP1, DSC1, DSCR1, MCIP1, RCN1, Down syndrome candidate region 1|Down syndrome critical region gene 1|calcipressin 1|calcium and oxidant-inducible mRNA|modulatory calcineurin-interacting pr
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.81 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Neuroscience
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This protein interacts with calcineurin A and inhibits calcineurin-dependent signaling pathways, possibly affecting central nervous system development. This gene is located in the minimal candidate region for the Down syndrome phenotype, and is overexpressed in the brain of Down syndrome fetuses. Chronic overexpression of this gene may lead to neurofibrillary tangles such as those associated with Alzheimer disease. Three transcript variants encoding three different isoforms have been found for this gene.
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse RCAN1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 86
Range % 82-90
1:2 Average % 101
Range % 98-104
1:4 Average % 95
Range % 91-99
1:8 Average % 87
Range % 83-90
Recovery
The recovery of mouse RCAN1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 106 103-109
EDTA plasma (n=4) 87 84-90
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
2000 2.548 2.601 2.575 2.451
1000 1.824 1.759 1.792 1.668
500 1.101 1.168 1.135 1.011
250 0.653 0.601 0.627 0.503
125 0.361 0.384 0.373 0.249
62.5 0.238 0.256 0.247 0.123
31.25 0.193 0.189 0.191 0.067
0 0.125 0.123 0.124  
References
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Function Inhibits calcineurin-dependent transcriptional responses by binding to the catalytic domain of calcineurin A. Could play a role during central nervous system development.
Protein Families RCAN family
Tissue Specificity Highly expressed in heart and skeletal muscle. Also expressed in all other tissues.
Database Links

KEGG: mmu:54720

STRING: 10090.ENSMUSP00000060394

UniGene: Mm.265744

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