Mouse Caspase-8(CASP8) ELISA kit

Instructions
Code CSB-EL004553MO
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Target Name caspase 8, apoptosis-related cysteine peptidase
Alternative Names Casp8Caspase-8 ELISA kit; CASP-8 ELISA kit; EC 3.4.22.61) [Cleaved into: Caspase-8 subunit p18; Caspase-8 subunit p10] ELISA kit
Abbreviation CASP8
Uniprot No. O89110
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 46.88 pg/mL-3000 pg/mL
Sensitivity 11.7 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cell Biology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse CASP8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 97
Range % 93-101
1:2 Average % 85
Range % 80-90
1:4 Average % 100
Range % 95-104
1:8 Average % 88
Range % 84-92
Recovery
The recovery of mouse CASP8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 95 90-100
EDTA plasma (n=4) 93 88-97
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
3000 2.712 2.601 2.657 2.493
1500 2.106 2.002 2.054 1.890
750 1.484 1.525 1.505 1.341
375 0.985 0.934 0.960 0.796
187.5 0.593 0.583 0.588 0.424
93.75 0.322 0.340 0.331 0.167
46.88 0.241 0.247 0.244 0.080
0 0.162 0.166 0.164  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Earn $30 Amazon Card or 20μL/μg CUSABIO Trial Size Antibody. Details of rewards >>

Target Data

Function Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-|-AMC. Likely target for the cowpox virus CRMA death inhibitory protein.
Gene References into Functions
  1. These data demonstrate that caspase-8 functions in synovial antigen-presenting cells to regulate the response to inflammatory stimuli by controlling RIPK3 action, and this delicate balance maintains homeostasis within the joint. PMID: 28978351
  2. Results illustrate the temporal and spatial activation of caspase-8 and -3 in microglia/macrophages occurring upon ischemic stroke and suggest that the expression of these caspases could be used in neuropathological diagnostic work. PMID: 27566702
  3. inhibition of TAK1 triggered two caspase 8 activation pathways through the induction of RIP1-FADD-caspase 8 complex as well as FLIP cleavage/degradation. PMID: 27685625
  4. this study identifies a crucial role for caspase-8 in the development of allergic airway inflammation PMID: 27007676
  5. caspase-8-dependent apoptosis was linked to hepatocellular carcinoma development. PMID: 28898696
  6. Statistically significant increases in the expression of Fas and caspase-8 were observed, along with other molecules involved in the extrinsic apoptotic pathway such as Dapk1, Traf3, Tnsf12, Tnfrsf1A and Ripk1. PMID: 27709293
  7. Results suggest that caspase-8 could regulate receptor-interacting protein 3 (RIP3)-mediated necroptosis. PMID: 28039150
  8. we show that caspase-8 activity promotes cell-intrinsic cytokine expression, independent of its role in cell death in response to Yersinia infection PMID: 27737018
  9. ING4 might suppress proliferation and enhance apoptosis in human malignant melanoma cells by activating Fas-induced apoptosis in a caspase-8-dependent pathway. PMID: 27070489
  10. studies have uncovered the combined actions of the NLRP3 inflammasome and caspase 8 leading to IL-1beta maturation and the directionality of IL-1beta in driving disease inPstpip2(cmo)mice. PMID: 27071119
  11. Chronic TLR Stimulation Controls NLRP3 Inflammasome Activation through IL-10 Mediated Regulation of NLRP3 Expression and Caspase-8 Activation PMID: 26412089
  12. A20 targets caspase-8 and FADD to protect HTLV-I-infected cells. PMID: 26437781
  13. Data indicate that receptor-interacting serine-threonine kinase 3 (RIPK3) deletion prevents inflammatory phenotypes in CreLysM (lysozyme M) Casp8fl/fl (caspase 8) mice. PMID: 26471282
  14. CASP8 deletion did not affect the overall number of microglia expressing the pan specific microglia marker, Iba1 PMID: 26405176
  15. We observed that NDMP generation was dependent on the extrinsic caspase apoptotic pathway (caspase 3 and caspase 8), cAMP activation of PKA but not of Epac, and on activation of MLCK. PMID: 26707875
  16. Casp8-dependent migration of enterocytes is likely involved in intestinal physiology and inflammation-related pathophysiology. PMID: 25914458
  17. NLRP3 inflammasomes directly utilize caspase-8 as both a pro-apoptotic initiator and major IL-1beta-converting protease PMID: 26100631
  18. The non-receptor tyrosine kinase Tec controls assembly and activity of the noncanonical caspase-8 inflammasome. PMID: 25474208
  19. RIPK1 indirectly regulates caspase-8 activation, in part via interaction with the ER stress sensor IRE1. PMID: 25476903
  20. Procaspase-8 mutation also stimulated the growth of head and neck squamous cell carcinoma xenograft tumors. PMID: 24816188
  21. TNF-alpha induces caspase-8 activation leading to ROS production, PARP-1 activation, and ADP-ribose production in adult ventricular cardiomyocytes. PMID: 24802330
  22. RIPK3 is an unexpected positive regulator of caspase 8 activity that promotes IL-1beta maturation in bone marrow-derived dendritic cells PMID: 25567679
  23. DICER1/Alu RNA dysmetabolism induces Caspase-8-mediated cell death in age-related macular degeneration. PMID: 25349431
  24. an important role for the proapoptotic protease caspase-8 in promoting beta-glucan-induced cell death and NLRP3 inflammasome-dependent IL-1beta maturation. PMID: 25063877
  25. Caspase-8 promotes NLRP1/NLRP3 inflammasome activation and IL-1beta production in acute glaucoma. PMID: 25024200
  26. results demonstrate that cell-type specific differences in apoptosis resistance mediated by Casp8 deletion are of significant relevance for the outcome of chronic liver injury PMID: 23928400
  27. These results suggest that TRAIL inhibits VEGF-induced angiogenesis by increasing caspase-8 activity and subsequently decreasing non-apoptotic signaling functions of procaspase-8, without inducing caspase-3 activation and endothelial cell cytotoxicity. PMID: 24097299
  28. Uncontrolled TLR activation in a RIPK1-dependent manner is responsible for the enhanced functionality of caspase-8-deficient DCs, because deletion of the TLR-signaling mediator, MyD88, ameliorates systemic autoimmunity PMID: 24808358
  29. These results clarify the complex roles for RIPK1 in postnatal life and provide insights into the regulation of FADD-caspase-8 and RIPK3-MLKL signaling by RIPK1. PMID: 24813850
  30. caspase-8 and the RIP kinases are key regulators of macrophage cell death, NF-kappaB and inflammasome activation, and host resistance after Y. pestis infection PMID: 24799678
  31. RIPK1, FADD, and caspase-8 are required for YopJ-induced cell death and caspase-1 activation PMID: 24799700
  32. our results reveal FADD and caspase-8 as apical mediators of canonical and noncanonical Nlrp3 inflammasome priming and activation. PMID: 24453255
  33. neither the unfolded protein response transcription factors XBP1 and CHOP nor the TLR4 adaptor molecule MyD88 is necessary for caspase-8 activation. Instead, both caspase activation and IL-1beta production require the alternative TLR4 adaptor TRIF. PMID: 24489101
  34. Data indicate that caspase-8 activity is lost upon deletion of c-FLIPL, and p43FLIP rescues caspase-8 activity through Raf1, TRAF2, and RIPK1 association, augmenting ERK and NF-kappaB pathways. PMID: 24275659
  35. caspase-8 is an integral part of the inflammasome, and this extends the relevance of the inflammasome to cell types that do not express caspase-1. PMID: 23645208
  36. Data indicate that ASK1 activation stimulated the activity of the transcription factor FoxO3a, which increased the abundance of the apoptosis-promoting adaptor protein TRADD, leading to activation of caspase 8. PMID: 23982205
  37. In virus-infected cells, a Bax/Bak-independent pathway involves dsRNA-induced innate immune signaling via mitochondrial antiviral signaling (MAVS) and caspase-8. PMID: 24391214
  38. blockade of caspase-8 activity in T cells improves immunity to L. major infection by promoting increased T helper 1 and T helper 2 responses. PMID: 24072877
  39. Casp8 comprises a nonapoptotic function during liver regeneration by balancing RIP1, NF-kappaB, and JNK activation PMID: 23728913
  40. Caspase-8 is a target of miR-874 which is in turn regulated by Foxo3a. PMID: 23828572
  41. Deletion of Casp8 specifically in murine germinal-center (GC) B cells results in larger GCs & a delay in affinity maturation, demonstrating the importance of apoptosis in GC homeostasis & clonal selection. PMID: 24244021
  42. proapoptotic chemotherapeutic agents stimulate the caspase-8-mediated processing and release of IL-1beta, implicating direct effects of such drugs on a noncanonical inflammatory cascade that may modulate immune responses in tumor microenvironments. PMID: 24078693
  43. Although caspase-8 itself is important for pro-IL-1beta synthesis, we hypothesize that the process of caspase-8 recruitment to the inflammasome is not critical for pro-IL-1beta synthesis. PMID: 24123685
  44. Treatment with TNF-alpha induced formation of neutrophil-derived microparticle by activating caspase 8 and NF-kappaB. PMID: 23850678
  45. Selective ablation of Casp8 in hepatocytes ameliorates development of nonalcoholic steatohepatitis by modulating liver injury. PMID: 23339067
  46. Activated beta-catenin can facilitate endosomal trafficking of internalized TNF to suppress caspase-8 activation in colon cancer cells. PMID: 23264463
  47. Alveolar macrophage-derived tumor necrosis factor (TNF) is required for caspase-8-induced alveolar epithelial dysfunction. PMID: 23487422
  48. The FADD-CASPASE-8-cFLIP(L) complex functions in the survival of developing mouse embryos. PMID: 22675671
  49. DP T cell apoptosis following experimental T. cruzi acute infection is dependent on glucocorticoid stimulation, promoting caspase-8 and -9 activation PMID: 23159925
  50. These results uncover a new mechanism of caspase-8-mediated apoptosis induced by intracellular calcium overload that is dependent on the autophagy-related proteins LC3 and p62 upon hyperactivation of DEG/ENaC channels. PMID: 23239879

Show More

Hide All

Subcellular Location Cytoplasm
Protein Families Peptidase C14A family
Tissue Specificity Expressed in a wide variety of tissues. Highest expression in spleen, thymus, lung, liver and kidney. Lower expression in heart, brain, testis and skeletal muscle.
Database Links

KEGG: mmu:12370

STRING: 10090.ENSMUSP00000027189

UniGene: Mm.336851

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

© 2007-2020 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1