Mouse Caspase-9,Casp-9 ELISA Kit

Instructions
Code CSB-E08864m
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name Caspase-9,Casp-9
Alternative Names Casp9 ELISA kit; Mch6Caspase-9 ELISA kit; CASP-9 ELISA kit; EC 3.4.22.62 ELISA kit; Apoptotic protease Mch-6 ELISA kit; Apoptotic protease-activating factor 3 ELISA kit; APAF-3 ELISA kit; ICE-like apoptotic protease 6 ELISA kit; ICE-LAP6) [Cleaved into: Caspase-9 subunit p35; Caspase-9 subunit p10] ELISA kit
Abbreviation Casp-9
Uniprot No. Q8C3Q9
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 12.5 pg/mL-800 pg/mL
Sensitivity 3.12 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cell Biology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse Casp-9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 96
Range % 93-99
1:2 Average % 108
Range % 104-112
1:4 Average % 85
Range % 81-89
1:8 Average % 97
Range % 93-101
Recovery
The recovery of mouse Casp-9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 86 83-90
EDTA plasma (n=4) 107 101-112
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
800 2.007 2.102 2.055 1.920
400 1.307 1.402 1.355 1.220
200 0.851 0.838 0.845 0.710
100 0.515 0.502 0.509 0.374
50 0.307 0.312 0.310 0.175
25 0.215 0.232 0.224 0.089
12.5 0.167 0.169 0.168 0.033
0 0.134 0.136 0.135  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP) (By similarity).
Gene References into Functions
  1. Inhibition of caspase-9 aggravates acute liver injury through suppression of cytoprotective autophagy. PMID: 27580936
  2. inducible caspase-9 (iC9) safety switch can eliminate CD19.CAR-Ts in a dose-dependent manner, allowing either a selective containment of CD19.CAR-T expansion in case of CRS or complete deletion on demand granting normal B cell reconstitution. PMID: 28187946
  3. Inhibition of Caspase-9 restricted, while Apaf-1 promoted, Chlamydia pneumoniae infection in HEp-2, HeLa, and mouse epithelial fibroblast (MEF) cells. PMID: 26290316
  4. Casp6 knockout in YAC128 mice does not abolish the presence of the mutant HTT protein-586 fragment but ameliorates depression, body weight gain, IGF-1 and BDNF levels in YAC128 mice. PMID: 25583186
  5. study demonstrates that MMP-3 leads to caspase-9 activation and suggests that this occurs indirectly via a cytosolic protein, possibly involving Apaf-1 PMID: 25285627
  6. caspase-9 mediates Puma activation to determine the threshold for overcoming chemoresistance in cancer cells. PMID: 25356864
  7. Casp9, the initiator caspase of the intrinsic apoptotic cascade, has a role in murine fetal and adult hematopoiesis PMID: 25349173
  8. The mitochondrial apoptotic pathway mediated by caspase 9 is constitutively activated in oocytes. PMID: 23384561
  9. caspase-9 activity is up-regulated by zVAD-fmk and is involved in an amplification loop of etoposide-induced cell death at the mitochondrial level in mouse embryonic fibroblasts. PMID: 22613767
  10. Both megakaryocytes and platelets possess a functional apoptotic caspase cascade downstream of Bcl-2 family-mediated mitochondrial damage. Caspase-9 is the initiator caspase, and its loss blocks effector caspase activation. PMID: 22294729
  11. Results identify a cancer-specific mechanism of hnRNP L phosphorylation and subsequent lowering of the caspase-9a/9b ratio, which is required for the tumorigenic capacity of non-small cell lung cancer cells. PMID: 20972334
  12. Findings suggest that Apaf-1/caspase-9-mediated nonapoptotic caspase signaling is required for the proper neural network formation during olfactory development. PMID: 20624980
  13. Alix is a crucial mediator of Ca(2+) induced caspase 9 activation. PMID: 20471954
  14. Processing of caspase 9 is induced in mouse macrophages upon phagocytosis of low-virulence Escherichia coli bacteria, activating the mitochondrial branch of the apoptotic pathway. PMID: 12218135
  15. In a permanent middle cerebral artery occlusion model, caspase 9 remains activated, signaling a sustained stimulation of the apoptotic cascade in the brain; procaspase 9L and procaspase 9S remain increased and decreased respectively. PMID: 12646305
  16. Caspase-9 is an upstream caspase in kidney organogenesis and cell death in nondifferentiating mesenchyme. PMID: 12660414
  17. DNA damage-induced decrease of Bcl-2 is secondary to the activation of Casp9. PMID: 14534531
  18. plays an important role in mutant SOD1-related neurodegeneration. PMID: 14657037
  19. Apaf-1 and caspase-9 do not suppress c-myc-induced lymphomagenesis and embryo fibroblast transformation. PMID: 14709542
  20. Coupling of caspase-9 to Apaf1 in apoptotic pathways is cell-type-specific. PMID: 14713951
  21. procaspase-9 is dimerized and processed by redox stress in mitochondria PMID: 14747474
  22. Loss of Apaf-1 or caspase-9 would be unlikely to enhance survival of cancer cells. PMID: 15210730
  23. Culture of oocytes in staurosporine increased the activity of caspase-8 and caspase-9. PMID: 15214581
  24. Apaf-1/pro-caspase-9 complex is recruited by Nucling for the induction of stress-induced apoptosis PMID: 15271982
  25. caspase-9 expression is regulated by the transcription factor activator protein-4 in glucocorticoid-induced apoptosis PMID: 15944155
  26. activated caspase-9 prevents the accessibility of cytochrome c to complex III, resulting in the production of reactive oxygen species PMID: 16007191
  27. HAX-1 is a newly identified anti-apoptotic factor and its mechanism of action is through caspase-9 inhibition. PMID: 16857965
  28. Results indicate that caspase-9 plays a dual role in cell death signaling, as an activator of effector caspases and lysosomal membrane permeabilization. PMID: 16966373
  29. Caspase 9 activation in response to intrinsic apoptotic stimuli can be uncoupled from Apaf-1 in vivo by XIAP antagonists. PMID: 17562856
  30. Apaf-1 and caspase-9 deficiency prevents apoptosis in a Bax-controlled pathway and promotes clonogenic survival during paclitaxel treatment. PMID: 17652622
  31. The protective effect of endocannabinoid levels on preneoplastic lesions appears to involve caspase-3, but not caspase-9 activation PMID: 17823781
  32. Mitochondria-cytochrome C-caspase-9 cascade mediates isorhamnetin-induced apoptosis in Lewis lung carcinoma. PMID: 18617322
  33. Sensitization to TRAIL protein is caspase-9 dependent and mediated in part via the mitochondrial pathway. PMID: 18802096
  34. Caspase-9 is responsible for the activation of caspase-3 in differentiating myoblasts cells. PMID: 18957517
  35. Regulation of apoptosis by Caspase-9 under oxidative stress conditions in mice testicular cells PMID: 18979186
  36. DYRK1A regulates caspase-9-mediated apoptosis during retina development PMID: 19081073
  37. Ihe functional interaction of Dyrk kinase and Mitogen-Activated Protein Kinase 14 to induce inhibitory caspase-9 phosphorylation provides a mechanism for the restraint of apoptosis in response to hyperosmotic stress. PMID: 19586613

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Protein Families Peptidase C14A family
Database Links

KEGG: mmu:12371

STRING: 10090.ENSMUSP00000030747

UniGene: Mm.88829

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