Mouse Cathepsin G(CTSG) ELISA kit

Instructions
Code CSB-EL006190MO
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name cathepsin G
Alternative Names Ctsg ELISA Kit; Cathepsin G ELISA Kit; EC 3.4.21.20 ELISA Kit; Vimentin-specific protease ELISA Kit; VSP ELISA Kit
Abbreviation CTSG
Uniprot No. P28293
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.78 ng/mL-50 ng/mL
Sensitivity 0.2 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse CTSG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 93
Range % 89-98
1:2 Average % 86
Range % 81-93
1:4 Average % 102
Range % 97-106
1:8 Average % 92
Range % 86-97
Recovery
The recovery of mouse CTSG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 96 90-101
EDTA plasma (n=4) 87 81-92
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
50 2.847 2.954 2.901 2.759
25 2.101 2.256 2.179 2.037
12.5 1.328 1.384 1.356 1.214
6.25 0.835 0.812 0.824 0.682
3.12 0.472 0.467 0.470 0.328
1.56 0.283 0.291 0.287 0.145
0.78 0.224 0.228 0.226 0.084
0 0.141 0.142 0.142  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

Target Data

Function This vimentin-specific protease may regulate the reorganization of vimentin filaments, occurring during cell differentiation, movement and mitosis.
Gene References into Functions
  1. these findings present evidence of an arterial-specific recruitment pattern centered on CatG-instructed adhesion strengthening. The inhibition of this process could provide a novel strategy for treatment of arterial inflammation with limited side effects. PMID: 27660294
  2. Cathepsin G activity may impair efferocytosis, which could lead to an accumulation of lesion-associated apoptotic cells and the accelerated progression of early atherosclerotic lesions to more complex lesions in Apoe(-/-) mice. PMID: 23934850
  3. SerpinB1 is critical for maintaining polymorphonuclear neutrophils survival by antagonizing intracellular cathepsin G activity. PMID: 23532733
  4. Cathepsin G-regulated release of formyl peptide receptor agonists modulate neutrophil effector functions. PMID: 22879591
  5. Cathepsin G and neutrophil elastase contribute to lung-protective immunity against mycobacterial infections in mice. PMID: 22461690
  6. This is the first study showing a contribution of neutrophil-derived neutral serine proteases CG and NE to lung-protective immunity against focal pneumonia-inducing serotype 19 S. pneumoniae in mice. PMID: 21911460
  7. In the absence of a pathogen challenge, neutrophil-derived proteases and nucleosomes contribute to large vessels thrombosis, the main trigger of myocardial infarction and stroke. PMID: 20676107
  8. Inhibition of TGF-beta significantly reduces microvessel density in mammary tumor-induced bone lesions, mediated by decreased expression of both vascular endothelial growth factor (VEGF) and monocyte chemotactic protein (MCP)-1. PMID: 19646811
  9. neutrophil elastase and cathepsin G are inhibited by PAI-1 mutants PMID: 15131125
  10. cathepsin G activity at the tumor-bone interface plays an important role in mammary tumor-induced osteolysis through RANKL PMID: 18632634
  11. both Cat-G and PAR(4) play key roles in generating and/or amplifying relapses in ulcerative colitis PMID: 19528350
  12. Cathepsin G and MMP9 were identified as proteases involved in enhanced TGF-beta signaling at the tumor-bone interface of mammary tumor-induced osteolytic lesions. PMID: 19671689
  13. Serine protease required for resistance to microbial infection; contributes to pathogenessis in endotoxic shock PMID: 10714686

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Subcellular Location Membrane
Protein Families Peptidase S1 family
Database Links

KEGG: mmu:13035

STRING: 10090.ENSMUSP00000015583

UniGene: Mm.4858

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