Mouse Cation-independent mannose-6-phosphate receptor(IGF2R) ELISA kit

Code CSB-EL011093MO
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. Q07113
Lead Time 5-7 working days
Abbreviation IGF2R
Protein Biological Process 1 Transport
Target Name insulin-like growth factor 2 receptor
Alias CD222, CIMPR, M6P-R, MPR1, MPRI, cation-independent mannose-6 phosphate receptor
Species Mus musculus (Mouse)
Protein Biological Process 3 Transport
Sample Types serum, plasma, tissue homogenates
Detection Range 3.12 pg/mL-200 pg/mL
Sensitivity 0.78 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This protein is a receptor for both, insulin-like growth factor 2 (IGF2) and mannose 6-phosphate (M6P). The IGF2 and M6P binding sites are located on different segments of the receptor. This receptor functions in the intracellular trafficking of lysosomal enzymes, the activation of transforming growth factor beta, and the degradation of IGF2. In mice, the homologous gene is expressed only from the maternal chromosome.
HGNC 5467
RGD 2871
MGI 96435
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse IGF2R in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:100 Average % 93
Range % 88-97
1:200 Average % 92
Range % 86-97
1:400 Average % 92
Range % 87-98
1:800 Average % 95
Range % 89-99
Recovery
The recovery of mouse IGF2R spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 103 96-107
EDTA plasma (n=4) 96 92-101
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
200 2.245 2.179 2.212 2.086
100 1.878 1.816 1.847 1.721
50 1.374 1.337 1.356 1.230
25 0.821 0.839 0.830 0.704
12.5 0.461 0.455 0.458 0.332
6.25 0.283 0.278 0.281 0.155
3.12 0.188 0.184 0.186 0.060
0 0.127 0.125 0.126  
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Function Acts as a positive regulator of T-cell coactivation, by binding DPP4 (By similarity). Transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex. This receptor also binds IGF2.
Subcellular Location Lysosome membrane, Single-pass type I membrane protein
Protein Families MRL1/IGF2R family
Database Links

KEGG: mmu:16004

STRING: 10090.ENSMUSP00000024599

UniGene: Mm.26553

Pathway Endocytosis

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