Mouse Chymase(CMA1) ELISA kit

Instructions
Code CSB-EL005599MO
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name chymase 1, mast cell
Alternative Names Cma1 ELISA kit; Mcpt5Chymase ELISA kit; EC 3.4.21.39 ELISA kit; Alpha-chymase ELISA kit; Mast cell chymase 1 ELISA kit; Mast cell protease 5 ELISA kit; mMCP-5 ELISA kit; Mast cell protease I ELISA kit
Abbreviation CMA1
Uniprot No. P21844
Species Mus musculus (Mouse)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse chymase in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 100
Range % 95-104
1:2 Average % 96
Range % 90-100
1:4 Average % 95
Range % 89-99
1:8 Average % 93
Range % 88-98
Recovery
The recovery of mouse chymase spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 96 89-101
EDTA plasma (n=4) 96 91-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
10 2.512 2.439 2.476 2.316
5 1.710 1.746 1.728 1.568
2.5 1.171 1.151 1.161 1.001
1.25 0.663 0.642 0.653 0.493
0.625 0.388 0.395 0.392 0.232
0.312 0.269 0.278 0.274 0.114
0.156 0.197 0.204 0.201 0.041
0 0.162 0.158 0.160  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Data

Function Major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion.
Gene References into Functions
  1. Mast cells have the ability to produce and degrade FXIIIA depending on their chymase expression profile: mast cells expressing chymase degrade FXIIIA, whereas mast cells that do not express chymase mainly produce FXIIIA PMID: 27302551
  2. Fibronectin was identified as a target of mMCP-5, and the exocytosis of mMCP-5 from the MCs in the mouse's peritoneal cavity resulted in the expression of metalloproteinase protease-9, which has been implicated in arthritis. PMID: 28193842
  3. mast cell/chymase-mediated intestinal epithelial barrier function is mediated by proteinase-activated receptor 2/MMP-2-dependent PMID: 23306080
  4. The prominent mast cell secretory granule protease MCP-5 is essential for the occurrence of irreversible ischemia-reperfusion injury of skeletal muscle. PMID: 15905575
  5. mast cell chymase activates ERK and p38 probably through G-protein-coupled receptor, and the ERK but not p38 cascade may have a crucial role in chymase-induced migration of eosinophils PMID: 15919053
  6. chymase rapidly cleaves recombinant pro-IL-18 at 56-phenylalanine and produces a biologically active IL-18 fragment that is smaller than any other reported IL-18-derived species PMID: 17142727
  7. Cymase might play an important role in intermittent hypoxia-induced left ventricular remodeling, which is independent of the systemic blood pressure. PMID: 19470876
  8. Just after stopping the angiotensin II infusion, aortic ACE and chymase activities were significantly increased, and were involved in the progression of aortic abdominal aneurysms PMID: 19556720
  9. experimental abdominal aortic aneurysm in wild-type mice or those deficient in the chymase ortholog mouse mMCP-4 or deficient in mMCP-5 (Mcpt4(-/-), Mcpt5(-/-)), Mcpt4(-/-) but not Mcpt5(-/-) had reduced abdominal aortic aneurysm formation PMID: 19720934

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Subcellular Location Secreted, Cytoplasmic granule
Protein Families Peptidase S1 family, Granzyme subfamily
Tissue Specificity Mast cells.
Database Links

KEGG: mmu:17228

STRING: 10090.ENSMUSP00000022834

UniGene: Mm.1252

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