Mouse Collectrin(TMEM27) ELISA kit

Code CSB-EL023823MO
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name transmembrane protein 27
Alternative Names Cltrn ELISA kit; Nx17 ELISA kit; Tmem27Collectrin ELISA kit; Transmembrane protein 27 ELISA kit
Abbreviation TMEM27
Uniprot No. Q9ESG4
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 62.5 pg/mL-4000 pg/mL
Sensitivity 15.6 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
To assess the linearity of the assay, samples were spiked with high concentrations of mouse TMEM27 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:1 Average % 86    
Range % 83-89    
1:2 Average % 95    
Range % 91-99    
1:4 Average % 87    
Range % 83-91    
1:8 Average % 102    
Range % 98-106    
The recovery of mouse TMEM27 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
Sample Type Average % Recovery Range    
Serum (n=5) 85 81-89    
EDTA plasma (n=4) 104 100-108    
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
pg/ml OD1 OD2 Average Corrected    
4000 2.541 2.468 2.505 2.323    
2000 1.702 1.698 1.700 1.518    
1000 1.045 1.068 1.057 0.875    
500 0.644 0.668 0.656 0.474    
250 0.443 0.451 0.447 0.265    
125 0.322 0.312 0.317 0.135    
62.5 0.248 0.251 0.250 0.068    
0 0.181 0.183 0.182      
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Data

Function Regulator of SNARE complex function. Stimulator of beta cell replication.
Gene References into Functions
  1. this study shows that Tmem27-mediated cross-presentation supports intrahepatic adaptive antiviral immune responses and may lead to insights into the nature of how the liver acts as a primary site of CD8+ T cell activation PMID: 28159899
  2. Collectrin is a consequential link between the transport of l-arginine and endothelial nitric oxide synthase uncoupling in hypertension. PMID: 24048198
  3. Collectrin and ACE2 in renal and intestinal amino acid transport. PMID: 21814048
  4. Tmem27 is a pancreatic beta cell transmembrane protein that regulates cell growth of pancreatic islets PMID: 16330324
  5. Our data suggest that collectrin is a novel mediator of renal amino acid transport and may provide further insight into the pathogenesis of a number of human disease correlates. PMID: 16985211
  6. data identify collectrin as a key regulator of renal amino acid uptake PMID: 17167413
  7. Collectrin has a role in amino acid transpor in the kidney [review] PMID: 17693757
  8. functional association of mutant B(0)AT1 transporters with ACE2 and collectrin in intestine and kidney, respectively, participates in the phenotypic heterogeneity of Hartnup disorder PMID: 19185582
  9. These data indicate the involvement of mitochondrial TCA cycle intermediates, distal to pyruvate, in the regulation of collectrin protein expression in beta-cells. PMID: 19715677

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Subcellular Location Membrane, Single-pass type I membrane protein
Protein Families TMEM27 family
Tissue Specificity Kidney; collecting ducts. Pancreas; beta cells of islets.
Database Links

KEGG: mmu:57394

STRING: 10090.ENSMUSP00000015545

UniGene: Mm.143766


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