Mouse Delta-like protein 3(DLL3) ELISA kit

Instructions
Code CSB-EL006948MO
Size 96T,5×96T,10×96T
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Product Details

Target Name delta-like 3 (Drosophila)
Alternative Names Dll3Delta-like protein 3 ELISA Kit; Drosophila Delta homolog 3 ELISA Kit; Delta3 ELISA Kit; M-Delta-3 ELISA Kit
Abbreviation DLL3
Uniprot No. O88516
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.81 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Developmental Biology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse DLL3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:1 Average % 102    
Range % 94-106    
1:2 Average % 90    
Range % 83-94    
1:4 Average % 98    
Range % 91-102    
1:8 Average % 109    
Range % 91-103    
Recovery
The recovery of mouse DLL3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 105 96-109    
EDTA plasma (n=4) 97 89-101    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
pg/ml OD1 OD2 Average Corrected    
2000 2.589 2.654 2.622 2.449    
1000 2.093 2.035 2.064 1.891    
500 1.474 1.422 1.448 1.275    
250 0.913 0.894 0.904 0.731    
125 0.536 0.556 0.546 0.373    
62.5 0.406 0.417 0.412 0.239    
31.25 0.267 0.271 0.269 0.096    
0 0.171 0.175 0.173      
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

Target Data

Function Inhibits primary neurogenesis. May be required to divert neurons along a specific differentiation pathway. Plays a role in the formation of somite boundaries during segmentation of the paraxial mesoderm.
Gene References into Functions
  1. Structural deformities of the vertebral column and adjacent ribs in the pudgy mouse are caused by mutations in Dll3. Review. PMID: 27655002
  2. Dll3 overexpression promoted PI3K/Akt signaling through inhibiting Notch signaling in lung cancer. PMID: 28007595
  3. O-fucosylation of DLL3 is required for its function during somitogenesis. PMID: 25856312
  4. Intriguing changes are observed in the cranio-caudal borders of multifidus muscle in mutant Dll3 and Lfng models of idiopathic scoliosis. PMID: 22095884
  5. Dll3 has a unique function during T-cell development that is distinct from the role played by the other DSL ligands of Notch. PMID: 21151194
  6. Dll3 targets Notch1 for lysosomal degradation preventing Notch1 from undergoing post-translational processing. PMID: 21147753
  7. Axial skeletal defects caused by mutation in the spondylocostal dysplasia/pudgy gene Dll3 are associated with disruption of the segmentation clock within the presomitic mesoderm. PMID: 11923214
  8. DLL3 knockout mice have segmentation and neural defects PMID: 12141422
  9. spondylocostal dysostosis (SCD) is caused by mutation in Delta-like 3 (DLL3), Mesoderm posterior 2 (MESP2), and Lunatic fringe (LFNG); three genes that are components of the Notch signaling pathway. PMID: 17600782
  10. study reports that another Notch ligand, Dll3, is expressed in developing hair cells, in a pattern that overlaps that of Dll1 and Jag2; Dll3 may play a role in lateral inhibition similar to that of Dll1 and Jag2 PMID: 17823936
  11. study shows that genetic interactions between Notch1 and Dll3 result in vertebral segmental defects similar to those seen in congenital scoliosis; craniofacial anomalies not previously observed in Dll3 homozygous animals were identified PMID: 17849441
  12. Dll3 are expressed in the developing mouse eye and in retinal progenitor cell. PMID: 19191219
  13. a complex interplay of E-box binding proteins spatially and temporally regulate Dll3 levels during neural tube development. PMID: 19389376
  14. Data show that the ubiquitin ligase Huwe1 operates upstream of the N-Myc-DLL3-Notch pathway to control neural stem cell activity and promote neurogenesis. PMID: 19686682

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Involvement in disease A truncating mutation in Dll3 is the cause of the pudgy (pu) phenotype. Pudgy mice exhibit patterning defects at the earliest stages of somitogenesis. Adult pudgy mice present severe vertebral and rib deformities.
Subcellular Location Membrane, Single-pass type I membrane protein
Tissue Specificity Predominantly expressed in the neuroectoderm and paraxial mesoderm during embryogenesis.
Database Links

KEGG: mmu:13389

STRING: 10090.ENSMUSP00000103951

UniGene: Mm.12896

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