Mouse Dickkopf-related protein 2(DKK2) ELISA kit

Code CSB-EL006921MO
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. Q9QYZ8
Lead Time 5-7 working days
Abbreviation DKK2
Protein Biological Process 1 Signaling Pathway
Target Name dickkopf homolog 2 (Xenopus laevis)
Alias DKK-2, dickkopf 2|dickkopf homolog 2|dickkopf related protein-2
Species Mus musculus (Mouse)
Protein Biological Process 3 Wnt signaling pathway
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 23.5 pg/mL-1500 pg/mL
Sensitivity 5.86 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Stem Cells
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This gene encodes a protein that is a member of the dickkopf family. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. It can act as either an agonist or antagonist of Wnt/beta-catenin signaling, depending on the cellular context and the presence of the co-factor kremen 2. Activity of this protein is also modulated by binding to the Wnt co-receptor LDL-receptor related protein 6 (LRP6).
HGNC 2892
RGD 1308639
MGI 1890663
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse DKK2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 96
Range % 92-100
1:2 Average % 100
Range % 96-107
1:4 Average % 91
Range % 85-95
1:8 Average % 94
Range % 90-98
Recovery
The recovery of mouse DKK2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 90 85-94
EDTA plasma (n=4) 97 93-101
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
1500 2.306 2.468 2.387 2.201
750 1.597 1.505 1.551 1.365
375 1.045 1.013 1.029 0.843
187.5 0.614 0.654 0.634 0.448
94 0.399 0.412 0.406 0.220
47 0.333 0.327 0.330 0.144
23.5 0.285 0.278 0.282 0.096
0 0.182 0.189 0.186  
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Function Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.
Subcellular Location Secreted
Protein Families Dickkopf family
Database Links

KEGG: mmu:56811

STRING: 10090.ENSMUSP00000029665

UniGene: Mm.103593

Pathway Wnt signaling pathway

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