Mouse ELAV-like protein 1(ELAVL1) ELISA kit

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Code CSB-EL007592MO
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P70372
Lead Time 7-14 working days
Abbreviation ELAVL1
Target Name ELAV (embryonic lethal, abnormal vision, Drosophila)-like 1 (Hu antigen R)
Alias ELAV1, HUR, Hua, MelG, ELAV-like 1|Hu antigen R|embryonic lethal, abnormal vision, drosophila, homolog-like 1
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 25 pg/mL-1600 pg/mL
Sensitivity 6.25 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Epigenetics and Nuclear Signaling
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This protein is a member of the ELAVL protein family. This encoded protein contains 3 RNA-binding domains and binds cis-acting AU-rich elements. It destabilizes mRNAs and thereby regulates gene expression.
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse ELAVL1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 94
Range % 90-98
1:2 Average % 86
Range % 83-89
1:4 Average % 97
Range % 91-102
1:8 Average % 85
Range % 81-90
Recovery
The recovery of mouse ELAVL1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 103 100-106
EDTA plasma (n=4) 89 85-93
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
1600 2.796 2.703 2.750 2.623
800 2.383 2.401 2.392 2.265
400 1.736 1.803 1.770 1.643
200 1.216 1.189 1.203 1.076
100 0.659 0.672 0.666 0.539
50 0.436 0.445 0.441 0.314
25 0.243 0.251 0.247 0.120
0 0.128 0.126 0.127  
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Function RNA-binding protein that binds to the 3'-UTR region of mRNAs and increases their stability. Involved in embryonic stem cells (ESCs) differentiation
Subcellular Location Cytoplasm, Nucleus
Protein Families RRM elav family
Database Links

KEGG: mmu:15568

STRING: 10090.ENSMUSP00000096549

UniGene: Mm.119162

Pathway IL-17 signaling pathway
AMPK signaling

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