Mouse Endothelial lipase,EL ELISA Kit

Code CSB-E08219m
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name lipase, endothelial
Alternative Names Lipg ELISA Kit; Endothelial lipase ELISA Kit; EC 3.1.1.3 ELISA Kit; Endothelial cell-derived lipase ELISA Kit; EDL ELISA Kit
Abbreviation LIPG
Uniprot No. Q9WVG5
Species Mus musculus (Mouse)
Sample Types serum, plasma
Detection Range 6.25 ng/mL-400 ng/mL
Sensitivity 1.95 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse EL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:100Average %90
Range %85-97
1:200Average %102
Range %98-106
1:400Average %98
Range %94-102
1:800Average %88
Range %84-93
Recovery
The recovery of mouse EL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9589-99
EDTA plasma (n=4)9790-101
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2Average
5000.113 0.121 0.117
2500.249 0.256 0.253
1250.472 0.491 0.482
62.50.809 0.785 0.797
31.21.268 1.224 1.246
15.61.722 1.829 1.776
7.81.929 2.029 1.979
02.370 2.497 2.434
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function Has phospholipase and triglyceride lipase activities. Hydrolyzes high density lipoproteins (HDL) more efficiently than other lipoproteins. Binds heparin (By similarity).
Gene References into Functions
  1. FoxA1, FoxA2, and LIPG control the uptake of extracellular lipids for breast cancer growth. PMID: 27045898
  2. inhibitory activity of AOE on pancreatic lipase enzyme was evaluated at concentrations from 60 to 1000 mug/mL. The AOE inhibited the pancreatic lipase with an IC50 = 588.5 mug/mL PMID: 26078966
  3. Hepatic lipase and endothelial lipase influence molecular species of several classes of plasma lipids. PMID: 24777581
  4. Furin has a role as the primary in vivo convertase of ANGPTL3 and endothelial lipase in hepatocytes PMID: 23918928
  5. The role of EL in HDL-associated S1P effects provides new insights into EL action, the responses seen through EL and HDL interaction, and S1P signaling. PMID: 23723371
  6. Endothelial lipase may act as an alternative candidate to provide fatty acids to the heart and regulate cardiac function. PMID: 23460280
  7. Hepatic lipase- and endothelial lipase-deficiency in mice promotes macrophage-to-feces RCT and HDL antioxidant properties. PMID: 23328279
  8. Endothelial lipase supplies cells with free fatty acids and HDL-derived lysophosphatidylcholine and lysophosphatidylethanolamine species resulting in increased cellular triglycerides and phosphatidylcholine (PC) content and decreased PC synthesis. PMID: 23075452
  9. EL expression modulates vascular remodeling as well as plasma HDL-C levels PMID: 22972429
  10. Starvation regulates endothelial lipase expression via SREBP-2. PMID: 23102786
  11. Endothelial lipase plays a role in thyroid and adipocyte biology in addition to its well-known role in endothelial function and HDL metabolism. PMID: 22740344
  12. DEHP exposure did not influence lipase activity, whereas it slightly enhanced UGT activity and exclusively increased CYP4A14 levels in pregnant and/or postpartum dams. PMID: 22159897
  13. targeted disruption of EL increased HDL particles with preserved anti-inflammatory and anti-atherosclerotic functions PMID: 20926433
  14. Statins can reduce EL expression in vitro and in vivo via inhibition of RhoA activity. The inhibition of EL expression in the vessel wall may contribute to the anti-atherogenic effects of statins. PMID: 20045866
  15. The additive effect of hepatic lipase and EL on HDL metabolism but not macrophage reverse cholesterol transport in mice. PMID: 20558822
  16. Data show that endothelial lipase (EL) was upregulated in rat atherosclerosis and taht lipopolysaccharide stimulates EL expression in vitro through NFkappaB activation. PMID: 19931348
  17. Endothelial lipase has a role in HDL metabolism [review] PMID: 12569156
  18. endothelial lipase has a role in regulating HDL levels PMID: 12569160
  19. endothelial lipase has a role in regulating HDL cholesterol levels PMID: 12569161
  20. EL is a major determinant of HDL concentration, structure, and metabolism in mice PMID: 12601178
  21. Human & mouse EL form specific protein-protein interactions, possibly as heterodimers. Inhibition of endogenous mEL by catalytically inactive human ELS149A may be the cause of decreased LDL-phospholipid turnover & increased lipid levels. PMID: 15117821
  22. EL may have indirect atherogenic actions in vivo through its effect on circulating HDL cholesterol and direct atherogenic actions through vascular wall processes such as monocyte recruitment and cholesterol uptake PMID: 15304490
  23. results suggest that endothelial lipase (EL) on the endothelial cell surface can promote monocyte adhesion to the vascular endothelium through the interaction with heparan sulfate proteoglycans PMID: 15485805
  24. The Endothelial lipase (EL) mRNA expression was high in ovaries from pregnant mice but low in ovaries from nonpregnant mice. PMID: 15576837
  25. endothelial lipase activity in adipose tissue and other peripheral tissues might contribute to the tissue uptake of free fatty acids, which could have important implications for the metabolism of plasma lipoproteins PMID: 15630456
  26. EL modulated lipoprotein profile in mice PMID: 16199802
  27. EL expression is upregulated by lipopolysaccharide via TLR4 and promotes the uptake of native LDL by macrophages PMID: 17726294
  28. after stimulation, the protease activity of PC5A is enhanced, as evidenced by the cleavage of the PC5A substrates Lefty, ADAMTS-4, endothelial lipase, and PCSK9. PMID: 18039650
  29. SR-BI-mediated selective uptake of HDL cholesteryl ester is essential for the remodeling of large alpha-migrating HDL particles by EL. PMID: 19136670
  30. Endothelial lipase is a novel modulator of the progression of allergic asthma. PMID: 19168574
  31. EL can modulate plasma HDL-CHOL levels through both its lipolytic and ligand-binding functions in hypertriglyceridemic mice, while lipolytic activity appears to be the main determinant for its effects on HDL metabolism in normolipidemic mice. PMID: 19672025

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Subcellular Location Secreted
Protein Families AB hydrolase superfamily, Lipase family
Tissue Specificity Expressed in placenta, lung, liver, testis and spleen.
Database Links

KEGG: mmu:16891

STRING: 10090.ENSMUSP00000066536

UniGene: Mm.299647

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