Mouse FMS-like tyrosine kinase 3,Flt3 ELISA Kit

Code CSB-E04557m
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name fms-related tyrosine kinase 3
Alternative Names Flt3 ELISA Kit; Flk-2 ELISA Kit; Flt-3Receptor-type tyrosine-protein kinase FLT3 ELISA Kit; EC 2.7.10.1 ELISA Kit; FL cytokine receptor ELISA Kit; Fetal liver kinase 2 ELISA Kit; FLK-2 ELISA Kit; Fms-like tyrosine kinase 3 ELISA Kit; FLT-3 ELISA Kit; Tyrosine-protein kinase receptor flk-2 ELISA Kit; CD antigen CD135 ELISA Kit
Abbreviation FLT3
Uniprot No. Q00342
Species Mus musculus (Mouse)
Detection Range Request Information
Sensitivity Request Information
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine FLT3LG and regulates differentiation, proliferation and survival of hematopoietic progenitor cells and of dendritic cells. Promotes phosphorylation of SHC1 and AKT1, and activation of the downstream effector MTOR. Promotes activation of RAS signaling and phosphorylation of downstream kinases, including MAPK1/ERK2 and/or MAPK3/ERK1. Promotes phosphorylation of FES, FER, PTPN6/SHP, PTPN11/SHP-2, PLCG1, and STAT5A and/or STAT5B. Activation of wild-type FLT3 causes only marginal activation of STAT5A or STAT5B. Mutations that cause constitutive kinase activity promote cell proliferation and resistance to apoptosis via the activation of multiple signaling pathways.
Gene References into Functions
  1. The demonstrated that an E2-2high fraction among common DC progenitors, which are a major source of pDCs and cDCs in the steady state, strictly gave rise to pDCs in the presence of Flt3 (Fms-like tyrosine kinase receptor-3) ligand ex vivo or in the secondary lymphoid organs when transferred in vivo. PMID: 29106601
  2. Acute myeloid leukemia tumor cell lines expressing the D835Y activation loop mutation of FLT3 failed to form colonies. PMID: 29372308
  3. Flt3 was heterogeneously expressed by almost all of the hematopoietic stem cell compartments. PMID: 28498310
  4. SLAP2 acts as a negative regulator of FLT3 signaling and therefore, modulation of SLAP2 expression levels may provide an alternative therapeutic approach for FLT3-ITD positive acute myeloid leukemia PMID: 27458164
  5. Tumor necrosis factor (TNF), a cell-extrinsic potent negative regulator of hematopoietic stem cells (HSCs), was overexpressed in bone marrow niche cells from FLT3 internal tandem duplications (FLT3 ITDs) mice. PMID: 28637883
  6. the angiogenic factor Egfl7 activates the Flt3/Flt3 ligand pathway and is a key molecular driver enforcing thymus progenitor generation and thereby directly links endothelial cell biology to the production of T cell-based adaptive immunity PMID: 28601636
  7. the Hoxa9- and Meis1-associated upregulation of Flt3 is a passive event with regard to leukemia development in mice and with limited relevance to the AML pathology. PMID: 27617578
  8. lineage-specific STAT5 activation in hematopoietic progenitor cells predicts the FLT3(+)-mediated leukemic phenotype in mice PMID: 27046463
  9. DOCK2 is a potential therapeutic target for novel AML treatments, as this protein regulates the survival of leukemia cells with elevated FLT3 activity and sensitizes FLT3/ITD leukemic cells to conventional antileukemic agents. PMID: 27748370
  10. Used a genetic model to determine whether miR-155 influences the development of FLT3-ITD-induced myeloproliferative disease. miR-155 promotes FLT3-ITD-induced myeloid expansion in the bone marrow, spleen, and peripheral blood. Mechanistically, miR-155 increases proliferation of the hematopoietic stem and progenitor cell compartments by reducing the growth-inhibitory effects of the interferon response. PMID: 28432220
  11. Overexpression of Abl-related gene tyrosine kinase ABL2 in pro-B cell line Ba/F3 cells expressing an oncogenic mutant of FLT3 (FLT3-ITD) resulted in partial inhibition of FLT3-ITD-dependent cell proliferation. PMID: 28086240
  12. Sorafenib-resistant leukemia cells with a FLT3/ITD mutation are sensitive to glycolytic inhibitors. PMID: 27132990
  13. Data indicate that most Fms-like tyrosine kinase-3 (FLT3) tyrosine kinase inhibitors (TKI) effectively target wild-type FLT3 signaling. PMID: 28077790
  14. murine Fms-like tyrosine kinase 3 (Flt3) ligand was used to generate conventional dendritic cells (FL-cDCs) and plasmacytoid DCs (FL-pDCs) and further evaluated their immunological responses to bacillus Calmette-Guerin (BCG) infection in vitro. PMID: 27496194
  15. increased number of osteoclasts in Flt3L(-/-) mice may be a consequence of insufficient expression of interferon regulatory factor 8 PMID: 26392589
  16. FLT3-tandem duplication mutation directly affects dendritic cell development, indirectly modulating T cell homeostasis and supporting T reg cell expansion. PMID: 26903243
  17. functional synergy between Flt3 and IL-4R signaling is critical for Stat-mediated regulation of sterile gamma1 germ-line transcripts and CSR to IgG1 PMID: 26627255
  18. STAT5 activation by FLT3-ITD protects cells treated with the PI3K/Akt pathway inhibitors from apoptosis by maintaining Mcl-1 expression through the mTORC1/4EBP1/eIF4E pathway. PMID: 25826077
  19. LAMTOR2 has a role in regulating dendritic cell homeostasis through FLT3-dependent mTOR signalling PMID: 25336251
  20. The Ras-PI3Kgamma-Akt-mTOR signaling axis acts downstream of FLT3L signaling and is required for development of lung CD103(+)and CD11b(+) dendritic cells. This signaling network regulating tissue-specific DC development is mediated by FLT3. PMID: 26453378
  21. Accumulation of FLT3(+) CD11c (+) dendritic cells in psoriatic lesions and the anti-psoriatic effect of a selective FLT3 inhibitor PMID: 24895100
  22. Hes1 suppresses acute myeloid leukemia development through FLT3 repression. PMID: 25234168
  23. Myeloid leukemia induced by the MLL-rearranged genes are not dependent upon Flt3 signaling. PMID: 23977266
  24. In conclusion, we successfully established xenotransplantation model of human FLT3-ITD (mut) /NPM1 (-) CN-AML in NOD/SCID mice. PMID: 23771655
  25. expression of trFlt3 in p53/NHEJ mutant hematopoietic progenitor cells promoted development of clinically aggressive B-cell leukemia PMID: 24888589
  26. these results show that Flt3 regulates the proliferation, survival, and maintenance of developmental stage-specific hematopoietic progenitors that give rise to BCPs. PMID: 24444745
  27. Data sugggest that wild-type Flt3 plays a role in leukemia initiation in vivo, which is, however, not universal. PMID: 24269847
  28. Data indicate hat tyrosine kinase Flk2 (Flt3)deficiency results in decreased cellularity of multipotent, lymphoid, and myeloid progenitors. PMID: 24333663
  29. This in vivo model of Flt3/ITD+/NPMc+ leukemia closely recapitulates human disease and will therefore serve as a tool for the investigation of the biology of this common disease entity PMID: 24184354
  30. a double knock-in of Mll(PTD/wt) and Flt3(ITD/wt) mutations induces spontaneous AML with an increase in DNA methyltransferases (Dnmt1, 3a, and 3b) and global DNA methylation index PMID: 24085765
  31. loss of the survival advantage of the FL knockout group in the NUP98-HOX13 model suggests that adding a second mutation changes the effect of FL expression in the context of more aggressive disease PMID: 23759524
  32. there is an extremely early checkpoint in hematopoietic differentiation critical for B and T lymphopoiesis that can only be transversed through the cooperative action of Hoxa9 and Flt3 signaling. PMID: 23772038
  33. Increased binase cytotoxicity toward the cells, expressing FLT3 oncogene, suggests that, as in the case of FDC-P1 cells, transduced by KIT oncogene, the expression of an activated oncogene determines the sensitivity of cells to binase PMID: 23808162
  34. Phosphoproteome analyses reveal specific implications of Hcls1, p21-activated kinase 1 and Ezrin in proliferation of a myeloid progenitor cell line downstream of wild-type and ITD mutant Fms-like tyrosine kinase 3 receptors. PMID: 23017497
  35. FLT3-internal tandem duplication mutations are implicated in the evolution of leukemic transformation. PMID: 22958930
  36. Expression of survivin is a downstream event of Flt3 signaling. PMID: 23082191
  37. These pathological changes were ameliorated by Flt3 ligand treatment. PMID: 23039886
  38. demonstrated that over-expression of miR-16 mimics suppressed Pim-1 expression in FD-FLT3/ITD cells suggesting that increased miR-16 expression contributes to depletion of Pim-1 after FLT3 inhibition PMID: 22970245
  39. Flt3-ITD mutations are associated with radiation-induced acute myeloid leukemia. PMID: 22289922
  40. Mice expressing both the FLT3/ITD and NHD13 transgene developed acute myeloid leukemia with 100% penetrance PMID: 22323452
  41. PPARgamma agonists play a FLT3-dependent role in stress responses of hematopoietic progenitor cells. PMID: 21967974
  42. displays a negative effect on differentiation of microglia from bone marrow stem cells PMID: 21854582
  43. These data demonstrate that hematopoietic stem cell specification and maintenance are Flk2 independent, and that hematopoietic lineage separation occurs downstream of Flk2 upregulation. PMID: 21726834
  44. The WT FLT3 allele seemingly functions as a tumor suppressor, attenuating the function of the FLT3/ITD allele in leukemia harboring FLT3/ITD mutations. PMID: 21908433
  45. The mouse Flt3(ITD/ITD) myeloid phenotype is FLT3 ligand-independent. PMID: 21813452
  46. Inhibition of fms-like tyrosine kinase 3 alleviates experimental arthritis by reducing formation of dendritic cells and antigen presentation. PMID: 21771900
  47. FLT3 ligand(FL) leads to further activation of FLT3 mutants and is especially important in light of recent findings of elevated FL levels in acute myeloid leukemia patients in response to chemotherapy. PMID: 21516120
  48. Data show that Flt3 expression initiates in fully multipotent progenitors because in addition to lymphoid and granulocyte-monocyte progenitors, FLT3(-) Mk- and E-restricted progenitors are also highly labeled when Flt3-Cre fate mapping is applied. PMID: 21628405
  49. p90RSK2 is dispensable for BCR-Abl-induced myeloid leukemia, but may be required for pathogenesis and lineage determination in Flt3-internal tandem duplication-induced hematopoietic transformation. PMID: 21527514
  50. Data demonstrate that the Flt3-ITD confers sensitivity to Ara-C, but resistance to doxorubicin in a manner that depends on p53. PMID: 21288478

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Subcellular Location Membrane, Single-pass type I membrane protein, Endoplasmic reticulum lumen
Protein Families Protein kinase superfamily, Tyr protein kinase family, CSF-1/PDGF receptor subfamily
Tissue Specificity Hematopoietic stem and progenitor cell-enriched populations. Found in brain, placenta and testis.
Database Links

KEGG: mmu:14255

STRING: 10090.ENSMUSP00000039041

UniGene: Mm.194

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