Mouse Fibroblast Growth Factor 8(FGF8) ELISA Kit

Instructions
Code CSB-E15863m
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name fibroblast growth factor 8 (androgen-induced)
Alternative Names Fgf8 ELISA Kit; Aigf ELISA Kit; Fibroblast growth factor 8 ELISA Kit; FGF-8 ELISA Kit; Androgen-induced growth factor ELISA Kit; AIGF ELISA Kit; Heparin-binding growth factor 8 ELISA Kit; HBGF-8 ELISA Kit
Abbreviation FGF8
Uniprot No. P37237
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 6.25 pg/mL-400 pg/mL
Sensitivity 1.56 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse FGF8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 86
Range % 84-95
1:2 Average % 95
Range % 91-100
1:4 Average % 84
Range % 81-89
1:8 Average % 108
Range % 103-113
Recovery
The recovery of mouse FGF8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 91 87-96
EDTA plasma (n=4) 103 95-108
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
400 2.446 2.544 2.495 2.390
200 1.715 1.822 1.769 1.664
100 0.943 1.003 0.973 0.868
50 0.556 0.566 0.561 0.456
25 0.301 0.321 0.311 0.206
12.5 0.233 0.232 0.233 0.128
6.25 0.177 0.179 0.178 0.073
0 0.103 0.106 0.105  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function Plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. Required for normal brain, eye, ear and limb development during embryogenesis. Required for normal development of the gonadotropin-releasing hormone (GnRH) neuronal system. Plays a role in neurite outgrowth in hippocampal cells (By similarity). Cooperates with Wnt-1 in mouse mammary tumor virus-induced murine mammary tumorigenesis
Gene References into Functions
  1. mesenchymal cells of the skull are not fated to form bone, but can be forced into a chondrogenic fate through the manipulation of FGF8 signaling. PMID: 29752281
  2. THe Fgf8-Cre reporter expression in isthmic structures in mice include 'signature' isthmic structures in mice include the trochlear nucleus, the dorsal raphe nucleus, the microcellular tegmental nuclei, the pedunculotegmental nucleus, the vermis of the cerebellum, rostral parts of the parabrachial complex and locus coeruleus, and the caudal parts of the substantia nigra and VTA. PMID: 28510270
  3. Results indicate that perinatal fibroblast growth factor 8 (FGF8) signaling is important for the timing of the onset of anterior-dorsal glial fibrillary acidic protein expression in midline glial cells suggesting that FGF8 function regulates midline GFAP-IR glial cell development, which when disrupted by Fgf8 deficiency prevents the formation of the corpus callosum. PMID: 27291295
  4. These results indicate that the modulatory effects of SHH on BALB/c mouse metanephric explant cultures may involve the regulation of Fgf8 expression but not Fgf10 expression, which provides evidence for the functional role of Fgf proteins in renal morphogenesis. PMID: 27510750
  5. FGF-8 was revealed to suppress BMP-induced osteoblast differentiation through the ERK pathway and the effects were enhanced by TNF-alpha. PMID: 26409788
  6. Fgf8 expression is required for the continued postnatal development/maturation of the Vasopressin and CRH neurons in the Paraventricular nucleus. PMID: 26537115
  7. Deregulated FGF8 and Otx2/Gbx2 gene expression underlies cerebellar vermis hypoplasia in mouse model of CHARGE syndrome. PMID: 24368733
  8. Cre fate mapping in Fgf8 mutant embryos revealed novel functions of this gene in rostral patterning center progenitor development. Disruption resulted in aberrant progenitor number and distribution in the rostral telencephalon. PMID: 25889070
  9. Tfap2a-dependent changes in mouse facial morphology result in clefting that can be ameliorated by a reduction in Fgf8 gene dosage PMID: 25381013
  10. This study demonistrated that Fgf8- and Fgfr1/Fgf8-deficient mice diplay increased anxiety-like behavior and reductions in specific populations of serotonergic neurons in the brain. PMID: 24512770
  11. Scube3 may be a critical upstream regulator of fast fiber myogenesis by modulating fgf8 signaling during zebrafish embryogenesis PMID: 24849601
  12. Retinoic acid directly represses Fgf8 through a retinoic acid response element-mediated mechanism that promotes repressive chromatin PMID: 25053430
  13. Together our data demonstrates that Foxc1 - Fgf8 signaling regulates mammalian jaw patterning and provides a mechanistic basis for the pathogenesis of syngnathia PMID: 24385915
  14. Data indicate that Foxi3 mutants succumb to apoptosis from embryonic day 9.75 onwards, and this cell death correlates with a delay in expression of Fgf8 in branchial arch ectoderm. PMID: 24650709
  15. FGF8 is not sufficient to induce ectodermal progenitors of the olfactory pit to acquire neural fate. altered neurogenesis and lack of GnRH neuron specification after reduced Fgf8 expression reflected dysgenesis of the nasal region. PMID: 24336726
  16. regulation of higher order chromatin organisation during differentiation in the embryo can be uncoupled from the machinery that promotes transcription and, for the first time, identify FGF as an extrinsic signal that can direct chromatin compaction PMID: 23874217
  17. In Fuz mutants, the phenotype stems from dysregulated Gli processing, which in turn results in excessive craniofacial Fgf8 gene expression. PMID: 23806618
  18. plasma membrane binding of Fgf8, and most likely of the Fgf8 family members Fgf17 and Fgf18, to Cubn improves Fgf ligand endocytosis and availability to FgfRs, thus modulating Fgf signaling activity. PMID: 23592779
  19. Fgf8-related secondary organizers exert different polarizing planar instructions along the mouse anterior neural tube. PMID: 22792203
  20. Fgf8 dosage determines midfacial integration and polarity within the nasal and optic capsules PMID: 23201021
  21. FGF8 is required to maintain a population of progenitor cells in the epiblast that generates mesoderm and contributes to the stem cell population that is incorporated in the tailbud and required for axial elongation of the mouse embryo after gastrulation. PMID: 22954964
  22. study examined if female mice hypomorphic for Fgf8, Fgfr1, or both (compound hypomorphs) exhibited altered parameters of pubertal onset, estrous cyclicity, and fertility PMID: 22278983
  23. results suggest that in stimulation of proliferation FGF-8b not only promotes cell cycle progression through the G1 restriction point but also regulates key proteins involved in chromosomal segregation during mitosis and cytokinesis of breast cancer cells PMID: 22465097
  24. FGF8 functions as a transcriptional regulator after being translocated to the nucleus. PMID: 22404534
  25. This study unravels a crucial role of RFX3 during early brain development by indirectly regulating GLI3 activity, which leads to FGF8 upregulation and ultimately to disturbed distribution of guidepost neurons required for CC morphogenesis PMID: 22479201
  26. findings implicate FGF8 as an organizer signal, and its source in the rostromedial telencephalon as an organizer of the neocortical area map. PMID: 22623663
  27. Global expression of Fgf8 is required for early neural crest survival and differentiation of placode-derived sensory neurons. PMID: 22040872
  28. Data demonstrate that both Shh and Gli3 suppress a potential Fgf/Wnt signaling source in the forebrain. PMID: 21925158
  29. Fgf8, but not Ret, expression was essential to the outgrowth of the cranial mesonephric tubules from the Wolffian duct and to the development of major portions of the sex accessory tissues in the male reproductive tract. PMID: 22110055
  30. autoregulation of Fgfr1 and Fgfr3 expression by Fgf8 represents a mechanism by which FGF8 could fine-tune its regulatory actions PMID: 20405041
  31. FGF8b is able to induce fast growth in strongly hypoxic tumour microenvironment whereas VEGF-stimulated growth advantage is associated with improved perfusion and oxygenation of prostate tumour xenografts PMID: 21034500
  32. study concludes FGF-8 suppresses thrombospondin 1 (TSP-1) expression through 2 pathways, MEK1/2 and PI3K; repression of TSP-1 may be an important mechanism involved in induction of an angiogenic phenotype and growth of FGF-8-expressing breast cancer PMID: 20370578
  33. FGF8 signaling may contribute to the phenotypic maturation of a neuroendocrine system that originates within the diencephalon PMID: 21046478
  34. Data demonstrate that increased epithelial production of FGF-8b leads to strongly activated and atypical stroma, which precedes development of mPIN lesions and prostate cancer in the prostates of TG mice. PMID: 21076617
  35. findings reveal the distinct function of Gbx2 and Fgf8 in a stepwise process in the development of the compartment boundary at the midbrain-hindbrain border PMID: 21266408
  36. Fgf8 gene is transcriptionally targeted by Wnt/beta-catenin signaling during early facial and forebrain development. PMID: 21070765
  37. Forced Fgf8 expression modifies and partially rescues the outflow tract septation defects of Tbx1 mutants but only if there is some residual expression of Tbx1. PMID: 20807544
  38. regulates cerebellar and optic tectal development. (review) PMID: 21089306
  39. Fgf8 function is required for normal progression through the late fetal stages of lung development. PMID: 20727874
  40. Results support the hypothesis that patients with digenic mutations in Fgfr1/Fgf8, but not Fgfr3, may have a further reduction in the GnRH neuronal population compared to patients harbouring monogenic haploid mutations in Fgfr1 or Fgf8. PMID: 20553372
  41. FGF8 acts as a classic diffusible morphogen to pattern the neocortex. PMID: 20843859
  42. Fgf8 deletion in the mesoderm alone leads to pharyngeal arch artery phenotypes and that these vascular phenotypes are exacerbated by loss of Fgf10 function in the mesodermal core of the arches. PMID: 20035084
  43. Fgf8b-containing spliceforms, but not Fgf8a, are essential for the function of Fgf8 during the development of the midbrain and cerebellum PMID: 19968985
  44. study underlines the crucial role of a defined Fgf8 expression pattern controlling inner ear formation in vertebrates PMID: 19619645
  45. Data showed that Fgf8 signalling is both required and sufficient to induce rostral Cajal-Retzius cells. PMID: 20040495
  46. The expression from early streak stage to midgestation of Fgf8 is measured. PMID: 9651520
  47. Transgenic expression of FGF8 induces differentiation of pancreatic islet cells into hepatocytes and exocrine cells PMID: 11890684
  48. Fgf8, Spry2 and Sef belong to a synexpression group and may functionally interact during mouse embryonic development PMID: 11960706
  49. Fgf8 mutants described herein display the complete array of cardiovascular, glandular and craniofacial phenotypes seen in human deletion 22q11 syndromes PMID: 12223415
  50. the first evidence of a genetic link between Tbx1 and FGF signaling; the FGF8 locus might affect the penetrance of cardiovascular defects in individuals with chromosome 22q11 deletions involving TBX1. PMID: 12223416

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Subcellular Location Secreted
Protein Families Heparin-binding growth factors family
Tissue Specificity Absent in normal mammary glands and detected only in adult testis and ovary and in midgestational embryos.
Database Links

KEGG: mmu:14179

STRING: 10090.ENSMUSP00000026241

UniGene: Mm.4012

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