Mouse Fibroblast Growth Factor 8(FGF8) ELISA Kit

Instructions
Code CSB-E15863m
Size 96T,5×96T,10×96T

Product Details

Target Name fibroblast growth factor 8 (androgen-induced)
Alternative Names AIGF, HBGF-8, KAL6, MGC149376, androgen-induced growth factor|fibroblast growth factor 8
Abbreviation FGF8
Uniprot No. P37237
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 6.25 pg/mL-400 pg/mL
Sensitivity 1.56 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse FGF8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 86
Range % 84-95
1:2 Average % 95
Range % 91-100
1:4 Average % 84
Range % 81-89
1:8 Average % 108
Range % 103-113
Recovery
The recovery of mouse FGF8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 91 87-96
EDTA plasma (n=4) 103 95-108
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
400 2.446 2.544 2.495 2.390
200 1.715 1.822 1.769 1.664
100 0.943 1.003 0.973 0.868
50 0.556 0.566 0.561 0.456
25 0.301 0.321 0.311 0.206
12.5 0.233 0.232 0.233 0.128
6.25 0.177 0.179 0.178 0.073
0 0.103 0.106 0.105  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days
Still Have Questions? Leave a Message or Start an on-line Chat

Target Data

Function Plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. Required for normal brain, eye, ear and limb development during embryogenesis. Required for normal development of the gonadotropin-releasing hormone (GnRH) neuronal system. Plays a role in neurite outgrowth in hippocampal cells (By similarity). Cooperates with Wnt-1 in mouse mammary tumor virus-induced murine mammary tumorigenesis
Subcellular Location Secreted
Protein Families Heparin-binding growth factors family
Tissue Specificity Absent in normal mammary glands and detected only in adult testis and ovary and in midgestational embryos.
Database Links

KEGG: mmu:14179

STRING: 10090.ENSMUSP00000026241

UniGene: Mm.4012

Most popular with customers

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

Copyright © 2007-2018 www.cusabio.com CUSABIO TECHNOLOGY LLC All Rights Reserved.